LOCUS       sequence-235988-        8794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ZIKV protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8794)
  TITLE     Rehwinkel Lab plasmids
REFERENCE   2  (bases 1 to 8794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.3/NS1
FEATURES             Location/Qualifiers
     source          1..8794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2247..2450
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2401..2421
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2452..2470
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2473..2491
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2532..2556
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3655..3679)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3732..3773
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3797..4385
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3850..3870)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4268..4279)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4400..4716
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4567..4702
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4764..4811
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4830..5228
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5315..5548
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5620..5741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5657..5676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5711..5730
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5781..5916
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5936..5955)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5937..5955)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5965..5982)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5965..5981)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5974..5996)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6123..6578
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6210..6229)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6420..6441
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6604..6708
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6709..6777,6778..7569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6927..6946)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7740..8328
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8229..8248
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8482..8499
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8616..8637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8652..8657,8658..8675,8676..8682)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8690..8706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8695..8717
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8714..8730
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8714..8730
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8749..8769
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8751..8769
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcttacat aacttacggt aaatggcccg cctggctgac
     1981 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2041 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2101 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2161 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2221 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2281 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2341 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2401 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca
     2461 gtgatagaga tctccctatc agtgatagag atcgtcgact agtccagtgt ggtggaattc
     2521 tgcagatatc aacaagtttg tacaaaaaag caggctccga attcgccctt aggaggacag
     2581 ctatggatgt ggggtgctcg gtggacttct caaagaagga gacgagatgc ggtacagggg
     2641 tgttcgtcta taacgacgtt gaagcctgga gggacaggta caagtaccat cctgactccc
     2701 cccgtagatt ggcagcagca gtcaagcaag cctgggaaga tggtatctgc gggatctcct
     2761 ctgtttcaag aatggagaac atcatgtgga gatcagtaga aggggagctc aacgcaatct
     2821 tggaagagaa tggagttcaa ctgacggtcg ttgtgggatc tgtaaaaaac cccatgtgga
     2881 gaggtccaca gagattgccc gtgcctgtga acgagctgcc ccacggctgg aaggcttggg
     2941 ggaaatcgta cttcgtcaga gcagcaaaga caaataacag ctttgtcgtg gatggtgaca
     3001 cactgaagga atgcccactc gaacatagag catggaacag ctttcttgtg gaggatcatg
     3061 ggttcggggt atttcacact agtgtctggc tcaaggttag agaagattat tcattagagt
     3121 gtgatccagc cgttattgga acagctgtta aggggaagga ggctgtacac agtgatctag
     3181 gctactggat tgagagtgag aagaatgaca catggaggct gaagagggcc catctaatcg
     3241 agatgaaaac atgtgaatgg ccaaagtccc acacattgtg ggcagatgga atagaagaga
     3301 gtgatctgat cattcccaag tctttagctg ggccactcag ccatcacaat accagagagg
     3361 gctacaggac ccaaatgaaa gggccatggc acagtgaaga gcttgaaatt cggtttgagg
     3421 aatgcccggg cactaaggtc cacgtggagg aaacatgtgg aacaagagga ccatctctga
     3481 gatcaaccac tgcaagcgga agggtgatcg aggaatggtg ctgcagggag tgcacaatgc
     3541 ccccactgtc gttccgggct aaagatggct gttggtatgg aatggagata aggcccagga
     3601 aagaaccaga aagcaactta gtaaggtcag tggtgactgc aaagggcgaa ttcgacccag
     3661 ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc
     3721 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     3781 gttagtaatg atcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3841 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3901 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3961 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4021 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4081 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4141 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     4201 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4261 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4321 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4381 cccgcctggc gatggtaccg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4441 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     4501 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     4561 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4621 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     4681 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     4741 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     4801 tataatacga caaggtgagg aactaaacca tggccaagcc tttgtctcaa gaagaatcca
     4861 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     4921 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     4981 ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     5041 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     5101 gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca
     5161 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     5221 agggctaagc acaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     5281 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5341 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     5401 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5461 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5521 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     5581 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     5641 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     5701 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     5761 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5821 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     5881 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     5941 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     6001 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     6061 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     6121 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6181 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6241 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6301 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6361 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6421 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     6481 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     6541 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     6601 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6661 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6721 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6781 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6841 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6901 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     6961 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     7021 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     7081 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     7141 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     7201 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7261 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7321 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7381 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7441 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7501 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7561 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7621 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7681 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7741 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7801 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7861 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7921 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7981 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     8041 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     8101 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8161 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8221 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8281 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8341 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8401 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8461 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     8521 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     8581 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8641 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8701 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     8761 cactaaaggg aacaaaagct ggagctgcaa gctt
//
