LOCUS       sequence-235990-       10661 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology 
            arms..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 10661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1583
FEATURES             Location/Qualifiers
     source          1..10661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             661..966
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             1902..1922
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(1940..1973)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1983..2012
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3829..4152)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6070..6094
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6097..7122
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6097..7122
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7123..7147)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7500..7533)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7579..7710
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             7738..7761
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7765..7788
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7792..7815
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(7863..8168)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8440..8456)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8440..8456)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8453..8475)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8464..8480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8488..8494,8495..8512,8513..8518))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8533..8554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8671..8688)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8842..9430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8922..8941)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9601..10392,10393..10461))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10224..10243
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10462..10566)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10634..10652
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
      421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
      541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
      661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
     1021 gagcctcagg agcatcgatg agtaaaggag aagaattgtt cactggagtt gtcccaatcc
     1081 tcgtcgagct cgacggagac gtcaacggac acaagttctc cgtctccgga gagggagagg
     1141 gagacgccac ctacggaaag ctcaccctca agttcatctg caccaccgga aagctcccag
     1201 tcccatggcc aaccctcgtc accaccttct gctacggagt ccaatgcttc tcccgttacc
     1261 cagaccacat gaagcgtcac gacttcttca agtccgccat gccagaggga tacgtccaag
     1321 agcgtaccat cttcttcaag gtaagtttaa acatatatat actaactact gattatttaa
     1381 attttcagga cgacggaaac tacaagaccc gtgccgaggt caagttcgag ggagacaccc
     1441 tcgtcaaccg tatcgagctc aaggtaagtt taaacagttc ggtactaact aaccatacat
     1501 atttaaattt tcagggaatc gacttcaagg aggacggaaa catcctcgga cacaagctcg
     1561 agtacaacta caactcccac aacgtctaca tcatggccga caagcaaaag aacggaatca
     1621 aggtcaactt caaggtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     1681 ttcagatccg tcacaacatc gaggacggat ccgtccaact cgccgaccac taccaacaaa
     1741 acaccccaat cggagacgga ccagtcctcc tcccagacaa ccactacctc tccacccaat
     1801 ccgccctctc caaggaccca aacgagaagc gtgaccacat ggtcctcctc gagttcgtca
     1861 ccgccgccgg aatcacccac ggaatggacg agctctacaa ggagaatctg tactttcaat
     1921 ccggaaaggt aagtttaaaa taacttcgta tagcatacat tatacgaagt tattttcagg
     1981 gagagcaaaa gcttatctcc gaggaggacc tctaaggatg atcgacgcca acgtcgttga
     2041 attttcaaat tttaaatact gaatatttgt tttttttcct attatttatt tattctcttt
     2101 gtgttttttt tcttgctttc taaaaaatta attcaatcca aatctaaaca tttttttttc
     2161 tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt gcaagtttat
     2221 ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg gggaaacaca
     2281 caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc aattcagaaa
     2341 tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga aactagctgc
     2401 cctcgattgt ctatcttcca ttctcaccaa gtttcaacaa ctttattcag tcattcttct
     2461 ctggttcatt tcccaaccaa tcttcccgaa taaagcccat ctgtcctttc ggccacattc
     2521 ctcacctaca tccgtactcc ttatctcccg ttcttatcca gtcttttatc gtctctgcgt
     2581 ctcgcacact gtctcgtcgg taatgtaaaa aaaaaacaaa aaaaacttga aaacttgcgg
     2641 aaagatttcc taataaaagg agacgaatcc ggtaggaaat cgacaagtct ctgcttctcg
     2701 aagactaggt gagttacatt tctttggccg aattattttg aaatcctaaa tttgataatt
     2761 tcagagatgt ctgtaaaact tgcgtgttat gtgacggctt cggtcactgt cgccactctt
     2821 atggtatgct tcatgaccat gtcaaccatc tactcggagg ttgatggatt cagagagaag
     2881 cttgataccg agatgaatgt gttcagagtg agttaaatta tattttgaat tttaaataat
     2941 ttttaatttt ctagcaatct accaatggat tgtggaagga catagttgtc atcggaagat
     3001 ctagcaagcg tgtccgttgt caatatgaag agaccaacgc taccccaact ccacatgctg
     3061 atggatcccc atctgctcca ccaggtcaac caccagcagt tccaccagtc ttcaaccagc
     3121 caaagactcc aaatggagcc aatggaaatg gaccaacctg caactgcaat gctgataaca
     3181 agtgcccagc tggaccatcc ggaccaaagg gagttccagg agttccagga ctcgacggag
     3241 ttccaggact tgacggtgtt ccaggagttg gagctgatga tatcgctcca caacgcgagt
     3301 ctgtcggatg cttcacttgc ccacaaggac cagttggacc accaggagct cttggaagac
     3361 caggaccacg tggacttcca ggaccaagag gacaaaatgg aaacccagga agagatggac
     3421 aaccaggaca tccaggagag caaggatcat ccggtcaaat cggaaagatc ggagagccag
     3481 gaccaccagg agagaaggga cgcgacgccg agcatccaat cggaagacca ggaccaaagg
     3541 gaccaagagg agatcaagga ccaacaggac cagctggaca gaacggtctt cacggaccac
     3601 caggagagcc aggaaccgtt ggaccagaag gaccatctgg aaagcaagga cgtcaaggac
     3661 cagacggaac ccagggagag actggaccag acggaagacc aggaaaggat gccgagtact
     3721 gccagtgccc agacaagtct ccaccatcag aggctgtcaa cgccaaccgt ggatacagaa
     3781 atatctaaat tgttggtgtt ttctaataaa atatttgaga ttcagtgaga cttttttctt
     3841 ggcggcacaa taaagtttcc tctttttgtt gggtcactca acgaacacaa tacaaatatt
     3901 tgagcgctcg catttttctc aacctttcgg gtttaaagca cttcaaaata gaattgaaga
     3961 gagattgtga aagtacatca gacagaatca cattttgata gcattcactt cactcagatg
     4021 caaacttttc agtgactata aaaagtcata aaataataac attaaaaaag caaataaatt
     4081 agcgagagaa ttttttgaca aaaagaaaga agagtgatag agaagaaggg aatgcttgaa
     4141 aggattttgc atttatctta atcaccgtct tcgagaagac gaaccatggc gccagtttca
     4201 gaatccaagt ttctaatata gttcatcaca atgttgacgt tggtccaacc tccggcttgc
     4261 ataatctctg ggatggaaac tccagcgcga gccatgtcac gggcagctcc gacacgggcg
     4321 gaatgtccag accaagccaa gtaacgttgt ccggaatcat ccttggctcc gtagatgaga
     4381 cggtgggtag cttcgaagat tcctgaaaat ttattttcag cttattctta gctaatatgt
     4441 tgtttaaact taccttcaag agctcgagtc gagagttgtg atgttgcact cggggcagca
     4501 actccgttct tgcgaacacg acagaacagg tagttgtttg gatcatcggc gactccggaa
     4561 accgaaatcc agcgctcaac gagcttggta actccaaggg agagcgcttt ttcgactccg
     4621 gcagtcgaga ccagcgtttt tgtacgtccg atgtggatga gcattcttcc tccgtctgtg
     4681 cgactaatat ccttgacgcg aatgcgggca atttcggcga tacggagaag tgtgttgtaa
     4741 gcaattccga ggaaggcgag gtttcggatg tcctggcaac gatcggaatt ttccataaga
     4801 gaacggacct gatcgaagtc ggttctgaaa atttcgtcaa gtcttaggat tgaatacatt
     4861 aatatttaaa cttacctttc gaaggcgagg gcttgcttgg ctctctctcc agcatcaaca
     4921 ttttccttgc ggatacgtct cattacgaga gatacggcat tagaatctga tgggcgtgga
     4981 agtccagatc ggcgatggag catattgagc tgtccgagat gttgttggat cgtcttcacc
     5041 gccaatccac gagcttggag gtagagcaag tagtcacgaa cgtcttctgg ctcggctggg
     5101 aaccacttgc ggttgttaag cttgcaccag gcagcccaag aacggcacac tgagagaagc
     5161 atcttccagg tgtgctcgga gaaggcttgt ctgtcacgga acatatccat gaggttcttg
     5221 cgaacttcat cacttgtcgc gtcaactgga agtgctggaa gattttgatg aacagtgaga
     5281 agatttgaca tattttcgaa gttttttaga tgcactagaa caaagcgtgt tggcttcctc
     5341 tgagcccgct ttccttatat acccgcattc tgcagcctta cagaatgttc tagaaggtcc
     5401 tagatgcatt cgtttgaaaa tactcccggt gggtgcaaag agacgcagac ggaaaatgta
     5461 tctgggtctc tttattgtgt acactacttt tccatgtacc gaatgtgagt cgccctcctt
     5521 ttgcaacaag cagctcgaat gttctagaaa aaggtggaaa atagtataaa taccgttgaa
     5581 aataaatacc gaacaacatt tgctctaatt gtgaaattag aaatcttcaa actataatca
     5641 tgtcacttta ccactatttc cgtccagctc aacgttccgt ttttggtgat catttttgct
     5701 ttcgtcgtaa atctacacac gcgtctcttc cgtgcgagag tccaagccag cagccaaatt
     5761 cgttgactga gtattcaacg tttatacgtt gtcggcaacg agaaatagga aaatgcatcg
     5821 ggaaatgttc ttttttcgat tttttccaag gttttgacaa attttaccac gaattttgct
     5881 atgttttcaa ttaaaaaata tgttattcaa ctgtttctat gaggaaaata aggctttgca
     5941 tgtaattttc ttattcagca taatttttaa ttaatttgaa ttttctgtcc taacgtttat
     6001 tttgttttct tggttatgac tgatctgaaa ttaatttttg aattttaagg taatatgtca
     6061 ggcggtgccg caagtttgta caaaaaagca ggctccatga aaaagcctga actcaccgcg
     6121 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     6181 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     6241 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     6301 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     6361 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     6421 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     6481 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     6541 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     6601 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     6661 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     6721 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     6781 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     6841 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     6901 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     6961 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     7021 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     7081 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agacccagct ttcttgtaca
     7141 aagtgggtcc aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc
     7201 ctatttttgt tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag
     7261 tcacctctaa caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc
     7321 gaaaaaaatt gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg
     7381 ttctgtgtac acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa
     7441 aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttaa
     7501 taacttcgta tagcatacat tatacgaagt tattttcagg gagccggatc tgattacgga
     7561 tccggaggtg gcgggatgcc taaagatcca gccaaacctc cggccaaggc acaagttgtg
     7621 ggatggccac cggtgagatc ataccggaag aacgtgatgg tttcctgcca aaaatcaagc
     7681 ggtggcccgg aggcggcggc gttcgtgaag ggtggtggcg gttctggctc aggatctgat
     7741 tataaagacg atgacgataa gcgtgactac aaggacgacg acgacaagcg tgattacaag
     7801 gatgacgatg acaagagact agttcgacct gcagactggc tgtgtataag ggagcctgac
     7861 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg
     7921 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc
     7981 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacgggg gactttatct
     8041 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata
     8101 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta
     8161 aactgcattt caccagcccc tgttctcgtc ggcaaaagag ccgttcattt caataaaccg
     8221 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg
     8281 ggcttcattc tgcatggttg tgcttaccga accggagata ttgacatcat atatgccttg
     8341 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc
     8401 tttttgacat acttcgggtt cctaggaaat cgataaatgg tcatagctgt ttcctgtgtg
     8461 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8521 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8581 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8641 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8701 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8761 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8821 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8881 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8941 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9001 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9061 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9121 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9181 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9241 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9301 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9361 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9421 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9481 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9541 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9601 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9661 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9721 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9781 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9841 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9901 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9961 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10021 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10081 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10141 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10201 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10261 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10321 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10381 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10441 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10501 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10561 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    10621 attaacctat aaaaataggc gtatcacgag gccctttcgt c
//
