LOCUS       sequence-235991-       10664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  YPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology 
            arms..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 10664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1584
FEATURES             Location/Qualifiers
     source          1..10664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             661..966
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             1905..1925
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(1943..1976)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1986..2015
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3832..4155)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6073..6097
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6100..7125
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6100..7125
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7126..7150)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7503..7536)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7582..7713
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             7741..7764
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7768..7791
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7795..7818
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(7866..8171)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8443..8459)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8443..8459)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8456..8478)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8467..8483
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8491..8497,8498..8515,8516..8521))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8536..8557
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8674..8691)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8845..9433)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8925..8944)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9604..10395,10396..10464))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10227..10246
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10465..10569)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10637..10655
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
      421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
      541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
      661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
     1021 gagcctcagg agcatcgatg tcaaaaggag aagagttatt tacaggagtt gtacctatcc
     1081 tcgtcgagct cgacggagac gtcaacggac acaagttctc cgtctccgga gagggagagg
     1141 gagacgccac ctacggaaag ctcaccctca agctcctctg caccaccgga aagctcccag
     1201 tcccatggcc aaccctcgtc accaccctcg gatacggagt ccaatgcttc gcccgttacc
     1261 cagaccacat gaagcaacac gacttcttca agtccgccat gccagaggga tacgtccaag
     1321 agcgtaccat cttcttcaag gtaagtttaa acatatatat actaactaac cctgattatt
     1381 taaattttca ggacgacgga aactacaaga cccgtgccga ggtcaagttc gagggagaca
     1441 ccctcgtcaa ccgtatcgag ctcaaggtaa gtttaaacag ttcggtacta actaaccata
     1501 catatttaaa ttttcaggga atcgacttca aggaggacgg aaacatcctc ggacacaagc
     1561 ttgagtacaa ctacaactcc cacaacgtct acatcaccgc cgacaagcaa aagaacggaa
     1621 tcaaggccaa cttcaaggta agtttaaaca tgattttact aactaactaa tctgatttaa
     1681 attttcagat ccgtcacaac atcgaggacg gaggagtcca actcgccgac cactaccaac
     1741 aaaacacccc aatcggagac ggaccagtcc tcctcccaga caaccactac ctctcctacc
     1801 aatccaagct cttcaaggac ccaaacgaga agcgtgacca catggtcctc cttgagttcc
     1861 tcaccgccgc cggaatcacc gagggaatga acgagctcta caaggagaat ctgtactttc
     1921 aatccggaaa ggtaagttta aaataacttc gtatagcata cattatacga agttattttc
     1981 agggagagca aaagcttatc tccgaggagg acctctaagg atgatcgacg ccaacgtcgt
     2041 tgaattttca aattttaaat actgaatatt tgtttttttt cctattattt atttattctc
     2101 tttgtgtttt ttttcttgct ttctaaaaaa ttaattcaat ccaaatctaa acattttttt
     2161 ttctctttcc gtctcccaat tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt
     2221 tatttatctt ctcgctttca tttcattagg acgtgggggg aattggtgga agggggaaac
     2281 acacaaaagg atgatggaaa tgaaataagg acacacaata tgcaacaaca ttcaattcag
     2341 aaatatggag gaaggtttaa aagaaaacat aaaaatatat agaggaggaa ggaaactagc
     2401 tgccctcgat tgtctatctt ccattctcac caagtttcaa caactttatt cagtcattct
     2461 tctctggttc atttcccaac caatcttccc gaataaagcc catctgtcct ttcggccaca
     2521 ttcctcacct acatccgtac tccttatctc ccgttcttat ccagtctttt atcgtctctg
     2581 cgtctcgcac actgtctcgt cggtaatgta aaaaaaaaac aaaaaaaact tgaaaacttg
     2641 cggaaagatt tcctaataaa aggagacgaa tccggtagga aatcgacaag tctctgcttc
     2701 tcgaagacta ggtgagttac atttctttgg ccgaattatt ttgaaatcct aaatttgata
     2761 atttcagaga tgtctgtaaa acttgcgtgt tatgtgacgg cttcggtcac tgtcgccact
     2821 cttatggtat gcttcatgac catgtcaacc atctactcgg aggttgatgg attcagagag
     2881 aagcttgata ccgagatgaa tgtgttcaga gtgagttaaa ttatattttg aattttaaat
     2941 aatttttaat tttctagcaa tctaccaatg gattgtggaa ggacatagtt gtcatcggaa
     3001 gatctagcaa gcgtgtccgt tgtcaatatg aagagaccaa cgctacccca actccacatg
     3061 ctgatggatc cccatctgct ccaccaggtc aaccaccagc agttccacca gtcttcaacc
     3121 agccaaagac tccaaatgga gccaatggaa atggaccaac ctgcaactgc aatgctgata
     3181 acaagtgccc agctggacca tccggaccaa agggagttcc aggagttcca ggactcgacg
     3241 gagttccagg acttgacggt gttccaggag ttggagctga tgatatcgct ccacaacgcg
     3301 agtctgtcgg atgcttcact tgcccacaag gaccagttgg accaccagga gctcttggaa
     3361 gaccaggacc acgtggactt ccaggaccaa gaggacaaaa tggaaaccca ggaagagatg
     3421 gacaaccagg acatccagga gagcaaggat catccggtca aatcggaaag atcggagagc
     3481 caggaccacc aggagagaag ggacgcgacg ccgagcatcc aatcggaaga ccaggaccaa
     3541 agggaccaag aggagatcaa ggaccaacag gaccagctgg acagaacggt cttcacggac
     3601 caccaggaga gccaggaacc gttggaccag aaggaccatc tggaaagcaa ggacgtcaag
     3661 gaccagacgg aacccaggga gagactggac cagacggaag accaggaaag gatgccgagt
     3721 actgccagtg cccagacaag tctccaccat cagaggctgt caacgccaac cgtggataca
     3781 gaaatatcta aattgttggt gttttctaat aaaatatttg agattcagtg agactttttt
     3841 cttggcggca caataaagtt tcctcttttt gttgggtcac tcaacgaaca caatacaaat
     3901 atttgagcgc tcgcattttt ctcaaccttt cgggtttaaa gcacttcaaa atggaattga
     3961 agagagattg tgaaagtaca tcagacagaa tcacattttg atagcattca cttcactcag
     4021 atgcaaactt ttcagtgact ataaaaagtc ataaaataat aacattaaaa aagcaaataa
     4081 attagcgaga gaattttttg acaaaaagaa agaagagtga tagagaagaa gggaatgctt
     4141 gaaaggattt tgcatttatc ttaatcaccg tcttcgagaa gacgaaccat ggcgccagtt
     4201 tcagaatcca agtttctaat atagttcatc acaatgttga cgttggtcca acctccggct
     4261 tgcataatct ctgggatgga aactccagcg cgagccatgt cacgggcagc tccgacacgg
     4321 gcggaatgtc cagaccaagc caagtaacgt tgtccggaat catccttggc tccgtagatg
     4381 agacggtggg tagcttcgaa gattcctgaa aatttatttt cagcttattc ttagctaata
     4441 tgttgtttaa acttaccttc aagagctcga gtcgagagtt gtgatgttgc actcggggca
     4501 gcaactccgt tcttgcgaac acgacagaac aggtagttgt ttggatcatc ggcgactccg
     4561 gaaaccgaaa tccagcgctc aacgagcttg gtaactccaa gggagagcgc tttttcgact
     4621 ccggcagtcg agaccagcgt ttttgtacgt ccgatgtgga tgagcattct tcctccgtct
     4681 gtgcgactaa tatccttgac gcgaatgcgg gcaatttcgg cgatacggag aagtgtgttg
     4741 taagcaattc cgaggaaggc gaggtttcgg atgtcctggc aacgatcgga attttccata
     4801 agagaacgga cctgatcgaa gtcggttctg aaaatttcgt caagtcttag gattgaatac
     4861 attaatattt aaacttacct ttcgaaggcg agggcttgct tggctctctc tccagcatca
     4921 acattttcct tgcggatacg tctcattacg agagatacgg cattagaatc tgatgggcgt
     4981 ggaagtccag atcggcgatg gagcatattg agctgtccga gatgttgttg gatcgtcttc
     5041 accgccaatc cacgagcttg gaggtagagc aagtagtcac gaacgtcttc tggctcggct
     5101 gggaaccact tgcggttgtt aagcttgcac caggcagccc aagaacggca cactgagaga
     5161 agcatcttcc aggtgtgctc ggagaaggct tgtctgtcac ggaacatatc catgaggttc
     5221 ttgcgaactt catcacttgt cgcgtcaact ggaagtgctg gaagattttg atgaacagtg
     5281 agaagatttg acatattttc gaagtttttt agatgcacta gaacaaagcg tgttggcttc
     5341 ctctgagccc gctttcctta tatacccgca ttctgcagcc ttacagaatg ttctagaagg
     5401 tcctagatgc attcgtttga aaatactccc ggtgggtgca aagagacgca gacggaaaat
     5461 gtatctgggt ctctttattg tgtacactac ttttccatgt accgaatgtg agtcgccctc
     5521 cttttgcaac aagcagctcg aatgttctag aaaaaggtgg aaaatagtat aaataccgtt
     5581 gaaaataaat accgaacaac atttgctcta attgtgaaat tagaaatctt caaactataa
     5641 tcatgtcact ttaccactat ttccgtccag ctcaacgttc cgtttttggt gatcattttt
     5701 gctttcgtcg taaatctaca cacgcgtctc ttccgtgcga gagtccaagc cagcagccaa
     5761 attcgttgac tgagtattca acgtttatac gttgtcggca acgagaaata ggaaaatgca
     5821 tcgggaaatg ttcttttttc gattttttcc aaggttttga caaattttac cacgaatttt
     5881 gctatgtttt caattaaaaa atatgttatt caactgtttc tatgaggaaa ataaggcttt
     5941 gcatgtaatt ttcttattca gcataatttt taattaattt gaattttctg tcctaacgtt
     6001 tattttgttt tcttggttat gactgatctg aaattaattt ttgaatttta aggtaatatg
     6061 tcaggcggtg ccgcaagttt gtacaaaaaa gcaggctcca tgaaaaagcc tgaactcacc
     6121 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     6181 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     6241 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     6301 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
     6361 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     6421 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     6481 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     6541 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     6601 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     6661 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     6721 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     6781 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     6841 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     6901 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     6961 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     7021 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     7081 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagaccca gctttcttgt
     7141 acaaagtggg tccaattact cttcaacatc cctacatgct ctttctccct gtgctcccac
     7201 cccctatttt tgttattatc aaaaaacttc tcttaatttc tttgtttttt agcttctttt
     7261 aagtcacctc taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa
     7321 gtcgaaaaaa attgtgctcc ctccccccat taataataat tctatcccaa aatctacaca
     7381 atgttctgtg tacacttctt atgtttttta cttctgataa atttttttga aacatcatag
     7441 aaaaaaccgc acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt
     7501 taataacttc gtatagcata cattatacga agttattttc agggagccgg atctgattac
     7561 ggatccggag gtggcgggat gcctaaagat ccagccaaac ctccggccaa ggcacaagtt
     7621 gtgggatggc caccggtgag atcataccgg aagaacgtga tggtttcctg ccaaaaatca
     7681 agcggtggcc cggaggcggc ggcgttcgtg aagggtggtg gcggttctgg ctcaggatct
     7741 gattataaag acgatgacga taagcgtgac tacaaggacg acgacgacaa gcgtgattac
     7801 aaggatgacg atgacaagag actagttcga cctgcagact ggctgtgtat aagggagcct
     7861 gacatttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg
     7921 ctgagatcag ccacttcttc cccgataacg gagaccggca cactggccat atcggtggtc
     7981 atcatgcgcc agctttcatc cccgatatgc accaccgggt aaagttcacg ggggacttta
     8041 tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata
     8101 atatcactct gtacatccac aaacagacga taacggctct ctcttttata ggtgtaaacc
     8161 ttaaactgca tttcaccagc ccctgttctc gtcggcaaaa gagccgttca tttcaataaa
     8221 ccgggcgacc tcagccatcc cttcctgatt ttccgctttc cagcgttcgg cacgcagacg
     8281 acgggcttca ttctgcatgg ttgtgcttac cgaaccggag atattgacat catatatgcc
     8341 ttgagcaact gatagctgtc gctgtcaact gtcactgtaa tacgctgctt catagcatac
     8401 ctctttttga catacttcgg gttcctagga aatcgataac tggtcatagc tgtttcctgt
     8461 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8521 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8581 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8641 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8701 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8761 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8821 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8881 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8941 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9001 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9061 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9121 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9181 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9241 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     9301 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9361 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9421 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9481 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9541 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9601 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9661 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9721 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9781 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9841 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9901 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9961 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    10021 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10081 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10141 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10201 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10261 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10321 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10381 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10441 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10501 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10561 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10621 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
