LOCUS       sequence-236010-        3583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  monomeric photosensitizing fluorescent protein for 
            chromophore-assisted light inactivation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Takemoto K, Matsuda T, Sakai N, Fu D, Noda M, Uchiyama S, Kotera I, 
            Arai Y, Horiuchi M, Fukui K, Ayabe T, Inagaki F, Suzuki H, Nagai T
  TITLE     SuperNova, a monomeric photosensitizing fluorescent protein for 
            chromophore-assisted light inactivation.
  JOURNAL   Sci Rep. 2013;3:2629. doi: 10.1038/srep02629.
  PUBMED    24043132
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013"; volume: "3:2629. doi"; pages: " 10.1038/srep02629"
COMMENT     Sequence Label: SuperNova/pRSETB
FEATURES             Location/Qualifiers
     source          1..3583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        23..41
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             73..95
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             115..132
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             136..168
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(172..180,181..195)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(205..207,208..213,214..924)
                     /codon_start=1
                     /product="monomeric variant of the photosensitizing red 
                     fluorescent protein KillerRed (Takemoto et al., 2013)"
                     /label=SuperNova
                     /translation="MGSEVGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFN
                     VHAVCETGKLPMSWKPICHLIQYGEPFFARYPDGISHFAQECFPEGLSIDRTVRFENDG
                     TMTSHHTYELDDTCVVSRITVNCDGFQPDGPIMRDQLVDILPSETHMFPHGPNAVRQTA
                     TIGFTTADGGKMMGHFDSKMTFNGSRAIEIPGPHFVTIITKQTRDTSDKRDHVCQREVA
                     YAHSVPRITSAIGSDED"
     primer_bind     complement(987..1005)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1001..1048
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1145..1600
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1232..1251)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1442..1463
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1626..1730
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1731..1799,1800..2591)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1949..1968)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2762..3350
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3251..3270
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3504..3521
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caggatctcg atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc
       61 tctagaaata attttgttta actttaagaa ggagatatac atatgcgggg ttctcatcat
      121 catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac
      181 gacgatgacg ataaggatcc caccatgggt tcagaggtcg gccccgccct gttccagagc
      241 gacatgacct tcaaaatctt catcgacggc gaggtgaacg gccagaagtt caccatcgtg
      301 gccgacggca gcagcaagtt cccccacggc gacttcaacg tgcacgccgt gtgcgagacc
      361 ggcaagctgc ccatgagctg gaagcccatc tgccacctga tccagtacgg cgagcccttc
      421 ttcgcccgct accccgacgg catcagccat ttcgcccagg agtgcttccc cgagggcctg
      481 agcatcgacc gcaccgtgcg cttcgagaac gacggcacca tgaccagcca ccacacctac
      541 gagctggacg acacctgcgt ggtgagccgc atcaccgtga actgcgacgg cttccagccc
      601 gacggcccca tcatgcgcga ccagctggtg gacatcctgc ccagcgagac ccacatgttc
      661 ccccacggcc ccaacgccgt gcgccagacc gccaccatcg gcttcaccac cgccgacggc
      721 ggcaagatga tgggccactt cgacagcaag atgaccttca acggcagccg cgccatcgag
      781 atccccggcc cacacttcgt gaccatcatc accaagcaga cgagggacac tagcgacaag
      841 cgcgaccacg tgtgccagcg cgaggtggcc tacgcccaca gcgtgccccg catcaccagc
      901 gccatcggta gcgacgagga ttaagaattc gaagcttgat ccggctgcta acaaagcccg
      961 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     1021 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatctggcgt
     1081 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     1141 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     1201 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     1261 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     1321 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     1381 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     1441 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     1501 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     1561 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     1621 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1681 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1741 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1801 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1861 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1921 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     1981 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2041 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     2101 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2161 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2221 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     2281 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     2341 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     2401 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     2461 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2521 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2581 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2641 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2701 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2761 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2821 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2881 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2941 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3001 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3061 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3121 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3181 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3241 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3301 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3361 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3421 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3481 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3541 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atg
//
