LOCUS       sequence-236023-        3101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD for sigma38/RpoS (RNA polymerase 
            active at transition from exponential phase to stationary phase). 
            Please see Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3101)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C38m
FEATURES             Location/Qualifiers
     source          1..3101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..23
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(27..70)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    73..94
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    1117..1137
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1143..1160)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1314..1902)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1394..1413)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2073..2864,2865..2933))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2696..2715
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2934..3038)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
       61 ggatgatttc tggaattcgc ggccgcttct agagactagt gggtctcaaa tgatgagtca
      121 gaatacgctg aaagttcatg atttaaatga agatgcggaa tttgatgaga acggagttga
      181 ggtttttgac gaaaaggcct tagtagaaca ggaacccagt gataacgatt tggccgaaga
      241 ggaactgtta tcgcagggag ccacacagcg tgtgttggac gcgactcagc tttaccttgg
      301 tgagattggt tattcaccac tgttaacggc cgaagaagaa gtttattttg cgcgtcgcgc
      361 actgcgtgga gatgtcgcct ctcgccgccg gatgatcgag agtaacttgc gtctggtggt
      421 aaaaattgcc cgccgttatg gcaatcgtgg tctggcgttg ctggacctta tcgaagaggg
      481 caacctgggg ctgatccgcg cggtagagaa gtttgacccg gaacgtggtt tccgcttctc
      541 aacatacgca acctggtgga ttcgccagac gattgaacgg gcgattatga accaaacccg
      601 tactattcgt ttgccgattc acatcgtaaa ggagctgaac gtttacctgc gaaccgcacg
      661 tgagttgtcc cataagctgg accatgaacc aagtgcggaa gagatcgcag agcaactgga
      721 taagccagtt gatgacgtca gccgtatgct tcgtcttaac gagcgcatta cctcggtaga
      781 caccccgctg ggtggtgatt ccgaaaaagc gttgctggac atcctggccg atgaaaaaga
      841 gaacggtccg gaagatacca cgcaagatga cgatatgaag cagagcatcg tcaaatggct
      901 gttcgagctg aacgccaaac agcgtgaagt gctggcacgt cgattcggtt tgctggggta
      961 cgaagcggca acactggaag atgtaggtcg tgaaattggc ctcacccgtg aacgtgttcg
     1021 ccagattcag gttgaaggcc tgcgccgttt gcgcgaaatc ctgcaaacgc aggggctgaa
     1081 tatcgaagcg ctgttccgcg agtaaaggta gagacctact agtagcggcc gctgcaggct
     1141 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1201 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1261 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac
     1321 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1381 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1441 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1501 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1561 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1621 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1681 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1741 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1801 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1861 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1921 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1981 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     2041 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     2101 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     2161 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgcgaccca
     2221 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2281 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2341 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     2401 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2461 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2521 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2581 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2641 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     2701 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2761 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2821 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2881 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2941 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     3001 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     3061 cctgacgtct aagaaaccat tattatcatg acattaacct a
//
