LOCUS       sequence-236025-       17052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon for dox-inducible expression of the OK+9MS 
            (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming 
            cassette (with mCherry). KRAB-dCas9 is constitutively expressed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17052)
  AUTHORS   Kagawa H, Shimamoto R, Kim SI, Oceguera-Yanez F, Yamamoto T, 
            Schroeder T, Woltjen K
  TITLE     OVOL1 Influences the Determination and Expansion of iPSC 
            Reprogramming Intermediates.
  JOURNAL   Stem Cell Reports. 2018 Dec 28. pii: S2213-6711(18)30527-7. doi: 
            10.1016/j.stemcr.2018.12.008.
  PUBMED    30639212
REFERENCE   2  (bases 1 to 17052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2018 Dec 28. pii: S2213-6711(18)30527-7. doi: 
            10.1016/j.stemcr.2018.12.008."
COMMENT     Sequence Label: PB-TAC-OK+9MS-KRAB-dCas9
FEATURES             Location/Qualifiers
     source          1..17052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   706..768
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1140..1158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1177..1195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1214..1232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1251..1269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1288..1306
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1325..1343
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1362..1380
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1399..1417
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1439..1463
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1543..1567
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2720..2785
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4250..4309
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5705..5770
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     protein_bind    complement(6810..6834)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6876..7450,7451..7453,7454..7462)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7043..7060)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7270..7289
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7463..8173
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7608..7626)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7876..7895
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8185..8204)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8250..8305
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8304..8323)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        8742..9027
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        9029..9306
                     /label=chicken beta-actin promoter
     intron          9307..9534
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      9546..9555
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9582..9767
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             join(9789..9815,9816..9818,9819..12305,12306..12308,
                     12309..13892)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9789..13892
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             13896..13922
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             13902..13928
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             13941..13961
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13968..13988
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(14055..14072)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    14061..14268
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   14679..14713
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(14862..14880)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14901..14917)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14901..14917)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14914..14936)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14925..14941
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14949..14955,14956..14973,14974..14979))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14994..15015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15132..15149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15303..15891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15383..15402)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16062..16853,16854..16922))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16685..16704
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16923..17027)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctcggtattc
      661 acgacagcag gctgaataat aaaaaaatta gaaactatta tttaacccta gaaagataat
      721 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      781 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      841 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      901 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      961 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
     1021 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
     1081 cacatatcaa tattatgctc tcgttaatta actcgaggga tccaagctgg ccgcacgtct
     1141 ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat agagaaggta
     1201 cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc tccctatcag
     1261 tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc gacacgtctc
     1321 gagctcccta tcagtgatag agaaggtacg tctagaacgt ctccctatca gtgatagaga
     1381 agtcgacacg tctcgagctc cctatcagtg atagagaagg taccccctat ataagcagag
     1441 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     1501 gaagacaccg ggaccgatcc agcctccgcg gtggcggcca tcacaagttt gtacaaaaaa
     1561 gcaggctggc gccggaacca attcagtcga ctggatccgg taccgaattc gcccttaaaa
     1621 agcaggctcc accttcccca tggctggaca cctggcttca gacttcgcct tctcaccccc
     1681 accaggtggg ggtgatgggt cagcagggct ggagccgggc tgggtggatt ctcgaacctg
     1741 gctaagcttc caagggcctc caggtgggcc tggaatcgga ccaggctcag aggtattggg
     1801 gatctcccca tgtccgcccg catacgagtt ctgcggaggg atggcatact gtggacctca
     1861 ggttggactg ggcctagtcc cccaagttgg cgtggagact ttgcagcctg agggccaggc
     1921 aggagcacga gtggaaagca actcagaggg aacctcctct gagccctgtg ccgaccgccc
     1981 caatgccgtg aagttggaga aggtggaacc aactcccgag gagtcccagg acatgaaagc
     2041 cctgcagaag gagctagaac agtttgccaa gctgctgaag cagaagagga tcaccttggg
     2101 gtacacccag gccgacgtgg ggctcaccct gggcgttctc tttggaaagg tgttcagcca
     2161 gaccaccatc tgtcgcttcg aggccttgca gctcagcctt aagaacatgt gtaagctgcg
     2221 gcccctgctg gagaagtggg tggaggaagc cgacaacaat gagaaccttc aggagatatg
     2281 caaatcggag accctggtgc aggcccggaa gagaaagcga actagcattg agaaccgtgt
     2341 gaggtggagt ctggagacca tgtttctgaa gtgcccgaag ccctccctac agcagatcac
     2401 tcacatcgcc aatcagcttg ggctagagaa ggatgtggtt cgagtatggt tctgtaaccg
     2461 gcgccagaag ggcaaaagat caagtattga gtattcccaa cgagaagagt atgaggctac
     2521 agggacacct ttcccagggg gggctgtatc ctttcctctg cccccaggtc cccactttgg
     2581 caccccaggc tatggaagcc cccacttcac cacactctac tcagtccctt ttcctgaggg
     2641 cgaggccttt ccctctgttc ccgtcactgc tctgggctct cccatgcatt caaacgggga
     2701 tctcaaaatt gtcgctcctg tcaaacaaac tcttaacttt gatttactca aactggctgg
     2761 ggatgtagaa agcaatccag gtccaatgag gcagccacct ggcgagtctg acatggctgt
     2821 cagcgacgct ctgctcccgt ccttctccac gttcgcgtcc ggcccggcgg gaagggagaa
     2881 gacactgcgt ccagcaggtg ccccgactaa ccgttggcgt gaggaactct ctcacatgaa
     2941 gcgacttccc ccacttcccg gccgccccta cgacctggcg gcgacggtgg ccacagacct
     3001 ggagagtggc ggagctggtg cagcttgcag cagtaacaac ccggccctcc tagcccggag
     3061 ggagaccgag gagttcaacg acctcctgga cctagacttt atcctttcca actcgctaac
     3121 ccaccaggaa tcggtggccg ccaccgtgac cacctcggcg tcagcttcat cctcgtcttc
     3181 cccagcgagc agcggccctg ccagcgcgcc ctccacctgc agcttcagct atccgatccg
     3241 ggccgggggt gacccgggcg tggctgccag caacacaggt ggagggctcc tctacagccg
     3301 agaatctgcg ccacctccca cggccccctt caacctggcg gacatcaatg acgtgagccc
     3361 ctcgggcggc ttcgtggctg agctcctgcg gccggagttg gacccagtat acattccgcc
     3421 acagcagcct cagccgccag gtggcgggct gatgggcaag tttgtgctga aggcgtctct
     3481 gaccacccct ggcagcgagt acagcagccc ttcggtcatc agtgttagca aaggaagccc
     3541 agacggcagc caccccgtgg tagtggcgcc ctacagcggt ggcccgccgc gcatgtgccc
     3601 caagattaag caagaggcgg tcccgtcctg cacggtcagc cggtccctag aggcccattt
     3661 gagcgctgga ccccagctca gcaacggcca ccggcccaac acacacgact tccccctggg
     3721 gcggcagctc cccaccagga ctacccctac actgagtccc gaggaactgc tgaacagcag
     3781 ggactgtcac cctggcctgc ctcttccccc aggattccat ccccatccgg ggcccaacta
     3841 ccctcctttc ctgccagacc agatgcagtc acaagtcccc tctctccatt atcaagagct
     3901 catgccaccg ggttcctgcc tgccagagga gcccaagcca aagaggggaa gaaggtcgtg
     3961 gccccggaaa agaacagcca cccacacttg tgactatgca ggctgtggca aaacctatac
     4021 caagagttct catctcaagg cacacctgcg aactcacaca ggcgagaaac cttaccactg
     4081 tgactgggac ggctgtgggt ggaaattcgc ccgctccgat gaactgacca ggcactaccg
     4141 caaacacaca gggcaccggc cctttcagtg ccagaagtgc gacagggcct tttccaggtc
     4201 ggaccacctt gccttacaca tgaagaggca ctttctcgag ggctcgggcc agtgtactaa
     4261 ttatgctctc ttgaaattgg ctggagatgt tgagagcaac ccaggtcccg gcgcgccact
     4321 ggatttcctt tgggcgttgg aaaccccgca gacagccacg acgatgcccc tcaacgtgaa
     4381 cttcaccaac aggaactatg acctcgacta cgactccgta cagccctatt tcatctgcga
     4441 cgaggaagag aatttctatc accagcaaca gcagagcgag ctgcagccgc ccgcgcccag
     4501 tgaggatatc tggaagaaat tcgagctgct tcccaccccg cccctgtccc cgagccgccg
     4561 ctccgggctc tgctctccat cctatgttgc ggtcgctacg tccttctccc caagggaaga
     4621 cgatgacggc ggcggtggca acttctccac cgccgatcag ctggagatga tgaccgagtt
     4681 acttggagga gacatggtga accagagctt catctgcgat cctgacgacg agaccttcat
     4741 caagaacatc atcatccagg actgtatgtg gagcggtttc tcagccgctg ccaagctggt
     4801 ctcggagaag ctggcctcct accaggctgc gcgcaaagac agcaccagcc tgagccccgc
     4861 ccgcgggcac agcgtctgct ccacctccag cctgtacctg caggacctca ccgccgccgc
     4921 gtccgagtgc attgacccct cagtggtctt tccctacccg ctcaacgaca gcagctcgcc
     4981 caaatcctgt acctcgtccg attccacggc cttctctcct tcctcggact cgctgctgtc
     5041 ctccgagtcc tccccacggg ccagccctga gcccctagtg ctgcatgagg agacaccgcc
     5101 caccaccagc agcgactctg aagaagagca agaagatgag gaagaaattg atgtggtgtc
     5161 tgtggagaag aggcaaaccc ctgccaagag gtcggagtcg ggctcatctc catcccgagg
     5221 ccacagcaaa cctccgcaca gcccactggt cctcaagagg tgccacgtct ccactcacca
     5281 gcacaactac gccgcacccc cctccacaag gaaggactat ccagctgcca agagggccaa
     5341 gttggacagt ggcagggtcc tgaagcagat cagcaacaac cgcaagtgct ccagccccag
     5401 gtcctcagac acggaggaaa acgacaagag gcggacacac aacgtcttgg aacgtcagag
     5461 gaggaacgag ctgaagcgca gcttttttgc cctgcgtgac cagatccctg aattggaaaa
     5521 caacgaaaag gcccccaagg tagtgatcct caaaaaagcc accgcctaca tcctgtccat
     5581 tcaagcagac gagcacaagc tcacctctga aaaggactta ttgaggaaac gacgagaaca
     5641 gttgaaacac aaactcgaac agcttcgaaa ctctggtgca ggggatctca aaattgtcgc
     5701 tcctgtcaaa caaactctta actttgattt actcaaactg gctggggatg tagaaagcaa
     5761 tccaggtcca ctcatgaaca attcgccctt caccatgtat aacatgatgg agacggagct
     5821 gaagccgccg ggcccgcagc aagcttcggg gggcggcggc ggaggaggca acgccacggc
     5881 ggcggcgacc ggcggcaacc agaagaacag cccggaccgc gtcaagaggc ccatgaacgc
     5941 cttcatggta tggtcccggg ggcagcggcg taagatggcc caggagaacc ccaagatgca
     6001 caactcggag atcagcaagc gcctgggcgc ggagtggaaa cttttgtccg agaccgagaa
     6061 gcggccgttc atcgacgagg ccaagcggct gcgcgctctg cacatgaagg agcacccgga
     6121 ttataaatac cggccgcggc ggaaaaccaa gacgctcatg aagaaggata agtacacgct
     6181 tcccggaggc ttgctggccc ccggcgggaa cagcatggcg agcggggttg gggtgggcgc
     6241 cggcctgggt gcgggcgtga accagcgcat ggacagctac gcgcacatga acggctggag
     6301 caacggcagc tacagcatga tgcaggagca gctgggctac ccgcagcacc cgggcctcaa
     6361 cgctcacggc gcggcacaga tgcaaccgat gcaccgctac gacgtcagcg ccctgcagta
     6421 caactccatg accagctcgc agacctacat gaacggctcg cccacctaca gcatgtccta
     6481 ctcgcagcag ggcacccccg gtatggcgct gggctccatg ggctctgtgg tcaagtccga
     6541 ggccagctcc agcccccccg tggttacctc ttcctcccac tccagggcgc cctgccaggc
     6601 cggggacctc cgggacatga tcagcatgta cctccccggc gccgaggtgc cggagcccgc
     6661 tgcgcccagt agactgcaca tggcccagca ctaccagagc ggcccggtgc ccggcacggc
     6721 cattaacggc acactgcccc tgtcgcacat gtgagggctg gaagggcgaa ttgcctgcag
     6781 gaattcgcgg ccgcactcga gatatctaga cccagctttc ttgtacaaag tggtgatcta
     6841 gctaaaacgc ggcctcgaat cgaattccgc ccccccccct ctccctcccc cccccctaac
     6901 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     6961 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     7021 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     7081 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     7141 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     7201 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     7261 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     7321 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     7381 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     7441 cgatgataat atggccacaa ccatggtgag caagggcgag gaggataaca tggccatcat
     7501 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     7561 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     7621 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     7681 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     7741 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     7801 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     7861 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     7921 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     7981 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     8041 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     8101 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     8161 gctgtacaag taaagcggcc gcgatctttt tccctctgcc aaaaattatg gggacatcat
     8221 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     8281 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     8341 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     8401 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     8461 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     8521 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     8581 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     8641 gcaggaattc gattacttaa gcctaggatc cagatctgta cattcgaatc gatgaattcg
     8701 ttaaccatgg aagcttctgc agcggccgca ctagaggtac ccgttacata acttacggta
     8761 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat agtaacgcca
     8821 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     8881 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     8941 cccgcctggc attgtgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     9001 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     9061 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     9121 gcagcgatgg gggcgggggg gggggggggg cgcgcgccrg gsggggsggg gsggggsgrg
     9181 gggsggggsg gggsgrggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga
     9241 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
     9301 cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc
     9361 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
     9421 tctcctccgg gctgtaatta gctgagcaag aggtaagggt ttaagggatg gttggttggt
     9481 ggggtattaa tgtttaatta cctggagcac ctgcctgaaa tcactttttt tcaggttgga
     9541 ccggtgccac catggatgct aagtcactaa ctgcctggtc ccggacactg gtgaccttca
     9601 aggatgtatt tgtggacttc accagggagg agtggaagct gctggacact gctcagcaga
     9661 tcgtgtacag aaatgtgatg ctggagaact ataagaacct ggtttccttg ggttatcagc
     9721 ttactaagcc agatgtgatc ctccggttgg agaagggaga agagcccggc ggttccggcg
     9781 gagggtcgat ggacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct
     9841 gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca
     9901 ccgaccggca cagcatcaag aagaacctga tcggcgccct gctgttcgac agcggagaaa
     9961 cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc
    10021 ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct
    10081 tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca
    10141 tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc
    10201 tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc
    10261 tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca
    10321 acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg
    10381 aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca
    10441 agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt
    10501 tcggcaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc
    10561 tggccgagga tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc
    10621 tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg
    10681 acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga
    10741 gcgcctctat gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc
    10801 tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg
    10861 gctacgccgg ctacatcgat ggcggagcca gccaggaaga gttctacaag ttcatcaagc
    10921 ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc
    10981 tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag
    11041 agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg
    11101 aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg
    11161 gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact
    11221 tcgaggaagt ggtggacaag ggcgccagcg cccagagctt catcgagcgg atgaccaact
    11281 tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact
    11341 tcaccgtgta caacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg
    11401 ccttcctgag cggcgagcag aaaaaagcca tcgtggacct gctgttcaag accaaccgga
    11461 aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg
    11521 tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc
    11581 tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag
    11641 atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa
    11701 cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg
    11761 gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga
    11821 caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc
    11881 acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg
    11941 atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc
    12001 tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga
    12061 acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc
    12121 gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag
    12181 aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga
    12241 atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg
    12301 tggacgctat cgtgcctcag agctttctga aggacgactc catcgataac aaagtgctga
    12361 ctcggagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga
    12421 agatgaagaa ctactggcgc cagctgctga atgccaagct gattacccag aggaagttcg
    12481 acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca
    12541 agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc
    12601 ggatgaacac taagtacgac gagaacgaca aactgatccg ggaagtgaaa gtgatcaccc
    12661 tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga
    12721 tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga
    12781 tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg
    12841 tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct
    12901 tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc
    12961 ggaagcggcc tctgatcgag acaaacggcg aaacaggcga gatcgtgtgg gataagggcc
    13021 gggactttgc caccgtgcgg aaagtgctgt ctatgcccca agtgaatatc gtgaaaaaga
    13081 ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgaca
    13141 agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca
    13201 ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga
    13261 agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc
    13321 ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc
    13381 tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg
    13441 gcgaactgca gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc
    13501 tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt
    13561 ttgtggaaca gcacaaacac tacctggacg agatcatcga gcagatcagc gagttctcca
    13621 agagagtgat cctggccgac gctaatctgg acaaggtgct gagcgcctac aacaagcaca
    13681 gagacaagcc tatcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc
    13741 tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca
    13801 gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga
    13861 cacggatcga cctgtctcag ctgggaggcg acgcctatcc ctatgacgtg cccgattatg
    13921 ccagcctggg cagcggctcc cccaagaaaa aacgcaaggt ggaagatcct aagaaaaagc
    13981 ggaaagtgga cggcattggt agtgggagca acggcagcag cggatcctaa gaattcctag
    14041 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
    14101 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
    14161 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
    14221 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagc ggccgcacta
    14281 gtctagtcta gagcgatcgc ccgggtttaa acgctagcca acaagctcgt catcgctttg
    14341 cagaagagca gagaggatat gctcatcgtc taaagaacta cccattttat tatatattag
    14401 tcacgatatc tataacaaga aaatatatat ataataagtt atcacgtaag tagaacatga
    14461 aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc
    14521 gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc gcattgacaa
    14581 gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca gcgacggatt
    14641 cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt ttacgcagac
    14701 tatctttcta gggttaaaaa agatttgcgc tttactcgac ctaaacttta aacacgtcat
    14761 agaatcttcg tttgacaaaa accacattgt ggccaagctg tgtgacgcga cgcgcgctaa
    14821 agaatggcaa accaagtcgc gcgaggtacc agcttttgtt ccctttagtg agggttaatt
    14881 tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    14941 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    15001 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    15061 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    15121 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    15181 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    15241 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    15301 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    15361 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    15421 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    15481 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    15541 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    15601 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    15661 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    15721 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    15781 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    15841 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    15901 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    15961 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    16021 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    16081 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    16141 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    16201 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    16261 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    16321 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    16381 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    16441 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    16501 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    16561 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    16621 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    16681 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    16741 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    16801 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    16861 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    16921 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    16981 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    17041 aaagtgccac ct
//
