LOCUS       sequence-236027-        2158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for blank part (random 50bp 
            sequence) - Please see Supplemental Documents for annotated Genbank 
            file.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2158)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C40m
FEATURES             Location/Qualifiers
     source          1..2158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..25
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(29..72)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    75..96
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    176..196
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(202..219)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(373..961)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(453..472)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1132..1923,1924..1992))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1755..1774
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1993..2097)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
       61 aaggatgatt tctggaattc gcggccgctt ctagagacta gtgggtctca aatgggccca
      121 tatctggaaa tagacgccaa gttcaatccg tattccgacg tacgaggtag agacctacta
      181 gtagcggccg ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      241 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      301 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      361 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      421 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      481 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      541 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      601 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      661 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      721 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      781 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      841 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      901 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      961 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1021 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1081 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1141 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1201 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1261 aatgataccg cgcgacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1321 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1381 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1441 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1501 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1561 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1621 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1681 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1741 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1801 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     1861 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     1921 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     1981 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2041 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2101 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaacc
//
