LOCUS       sequence-236029-        2825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for sfYFP (superfolder YFP; 
            variant of https://www.fpbase.org/protein/yfp/ - Please see 
            Supplemental Documents for annotated Genbank file.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2825)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C51m
FEATURES             Location/Qualifiers
     source          1..2825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     35..53
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(57..100)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    103..124
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             140..853
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    871..891
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(897..914)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1068..1656)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1148..1167)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1827..2618,2619..2687))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2450..2469
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2688..2792)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt
       61 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct
      121 agagactagt gggtctcaaa tgcgtaaagg cgaagagctg ttcactggtg tcgtccctat
      181 tctggtggaa ctggatggtg atgtcaacgg tcataagttt tccgtgcgtg gcgagggtga
      241 aggtgacgca actaatggta aactgacgct gaagttcatc tgtactactg gtaaactgcc
      301 ggtaccttgg ccgactctgg taacgacgct gacttatggt gttcagtgct ttgctcgtta
      361 tccggaccat atgaagcagc atgacttctt caagtccgcc atgccggaag gctatgtgca
      421 ggaacgcacg atttccttta aggatgacgg cacgtacaaa acgcgtgcgg aagtgaaatt
      481 tgaaggcgat accctggtaa accgcattga gctgaaaggc attgacttta aagaagacgg
      541 caatatcctg ggccataagc tggaatacaa ttttaacagc cacaatgttt acatcaccgc
      601 cgataaacaa aaaaatggca ttaaagcgaa ttttaaaatt cgccacaacg tggaggatgg
      661 cagcgtgcag ctggctgatc actaccagca aaacactcca atcggtgatg gtcctgttct
      721 gctgccagac aatcactatc tgagctacca aagcgttctg tctaaagatc cgaacgagaa
      781 acgcgatcat atggttctgc tggagttcgt aaccgcagcg ggcatcacgc atggtatgga
      841 tgaactgtac aaataatgaa ggtagagacc tactagtagc ggccgctgca ggcttcctcg
      901 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      961 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1021 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc
     1081 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1141 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1201 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1261 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1321 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1381 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1441 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1501 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1561 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1621 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1681 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1741 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1801 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1861 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1921 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgcga cccacgctca
     1981 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2041 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2101 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2161 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2221 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2281 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2341 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2401 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2461 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2521 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2581 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2641 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2701 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2761 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2821 gtcta
//
