LOCUS       sequence-236030-        2804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for mScarlet (RFP variant; see 
            https://www.fpbase.org/protein/mscarlet/). Please see Supplemental 
            Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2804)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C59m
FEATURES             Location/Qualifiers
     source          1..2804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     29..47
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(51..94)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    97..118
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             137..832
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(270..288)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    844..864
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(870..887)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1041..1629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1121..1140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1800..2591,2592..2660))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2423..2442
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2661..2765)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata
       61 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagac
      121 tagtgggtct caaatgatgg tgagcaaggg cgaggcagtg atcaaggagt tcatgcggtt
      181 caaggtgcac atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga
      241 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
      301 gcccttctcc tgggacatcc tgtcccctca gttcatgtac ggctccaggg ccttcaccaa
      361 gcaccccgcc gacatccccg actactataa gcagtccttc cccgagggct tcaagtggga
      421 gcgcgtgatg aacttcgagg acggcggcgc cgtgaccgtg acccaggaca cctccctgga
      481 ggacggcacc ctgatctaca aggtgaagct ccgcggcacc aacttccctc ctgacggccc
      541 cgtaatgcag aagaaaacaa tgggctggga agcgtccacc gagcggttgt accccgagga
      601 cggcgtgctg aagggcgaca ttaagatggc cctgcgcctg aaggacggcg gccgctacct
      661 ggcggacttc aagaccacct acaaggccaa gaagcccgtg cagatgcccg gcgcctacaa
      721 cgtcgaccgc aagttggaca tcacctccca caacgaggac tacaccgtgg tggaacagta
      781 cgaacgctcc gagggccgcc actccaccgg cggcatggac gagctgtaca agaggtagag
      841 acctactagt agcggccgct gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
      901 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      961 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1021 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
     1081 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1141 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1201 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1261 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1321 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1381 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1441 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1501 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1561 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1621 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1681 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1741 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1801 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1861 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1921 agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc agcaataaac
     1981 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2041 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2101 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2161 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2221 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2281 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2341 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     2401 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2461 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2521 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     2581 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     2641 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2701 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2761 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaac
//
