LOCUS       sequence-236031-        2840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for RpaR* (improved RpaR 
            quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). 
            Please see Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2840)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C61m
FEATURES             Location/Qualifiers
     source          1..2840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..33
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(37..80)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    83..104
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    866..886
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(892..909)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1063..1651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1143..1162)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1822..2613,2614..2682))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2445..2464
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2683..2787)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag
       61 ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agagactagt gggtctcaaa
      121 tgatgatcgt cggcgaagat cagctttggg gacggcgtgc gctggaattc gtcgattccg
      181 tcgaacggct cgaggcgccg gcgctgatca gccggttcga atcgctgatc gcgagctgcg
      241 gatttaccgc ctacatcatg gccggcctgc cgtcgcgcaa tgccggacta ccggagctga
      301 cgctggccaa tggctggccg cgagactggt tcgatctgta tgtcagcgaa aacttcagcg
      361 cggtcgatcc ggtgccgcgc cacggcgcta ccacggttca tcctttcgta tggtccgatg
      421 caccctacga ccgcgaccgt gatccggccg cccaccgggt catgacccgg gcggcggaat
      481 tcggactggt cgagggttac tgcattccgc tgcactacga cgacggtagc gccgcgatca
      541 gcatggccgg caaggatccg gacctcagcc cggccgcgcg cggcgcgatg cagctggtca
      601 gcatctacgc gcatagtcgc ctgcgcgcac tcagccggcc aaagccgatc cggcgcaacc
      661 ggctcacgcc gcgcgagtgc gagatcctgc aatgggcagc gcagggcaag accgcctggg
      721 aaatctcggt aatcctctgc atcaccgaac gcacggtgaa attccatctg atcgaagccg
      781 cccgcaagct cgacgccgcc aaccgcaccg cggcggttgc caaggcattg acgctcggat
      841 tgatccgttt gtgaaggtag agacctacta gtagcggccg ctgcaggctt cctcgctcac
      901 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      961 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1021 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc
     1081 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1141 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1201 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1261 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1321 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1381 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1441 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1501 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1561 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1621 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1681 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1741 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1801 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1861 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1921 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgcgacccac gctcaccggc
     1981 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2041 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2101 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2161 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2221 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2281 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2341 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     2401 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     2461 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2521 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     2581 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2641 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2701 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2761 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     2821 agaaaccatt attatcatga
//
