LOCUS       sequence-236032-        2813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for TraR(W) (improved TraR 
            quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). 
            Please see Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2813)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C62m
FEATURES             Location/Qualifiers
     source          1..2813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..50
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(54..97)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    100..121
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    856..876
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(882..899)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1053..1641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1133..1152)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1812..2603,2604..2672))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2435..2454
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2673..2777)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag
       61 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga
      121 gactagtggg tctcaaatga tgcagcactg gctggataag ctgactgatc tggcggcaat
      181 tgagggtgat gaatgcattc tgaaaactgg tctggcggat attgcggacc acttcggttt
      241 taccggttac gcgtacctgc acatccagca ccgtcatatt accgctgtga ccaactacca
      301 ccgtcaatgg caatccacct acttcgataa aaaattcgag gcgctggacc cggtggttaa
      361 gcgtgcgcgt tctcgcaaac acatcttcac ttggagcggt gaacacgaac gtccaactct
      421 gagcaaagac gaacgcgcgt tctacgacca cgcgtccgac tttggtatcc gttctggcat
      481 caccattccg attaaaacgg ccaacggttt catgtctatg ttcaccatgg caagcgataa
      541 accggttatc gacctggacc gtgaaatcga cgccgtagct gcggcggcga ccatcggtca
      601 aattcacgcg cgtatctcct ttctgcgcac gaccccgacg gcggaggacg ctgcatggct
      661 ggatcctaag gaagctactt acctgcgttg gatcgccgta ggcaaaacca tgtgggaaat
      721 cgcagatatc gaaggcgtta aatacaattc cgtccgcgta aaactgcgcg aagctatgaa
      781 acgcttcgac gtacgttcta aagcccacct gacggccctg gctatccgtc gcaagctgat
      841 ctgaaggtag agacctacta gtagcggccg ctgcaggctt cctcgctcac tgactcgctg
      901 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      961 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1021 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag
     1081 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1141 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1201 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1261 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1321 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1381 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1441 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1501 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1561 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1621 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1681 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1741 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1801 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1861 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1921 ccatctggcc ccagtgctgc aatgataccg cgcgacccac gctcaccggc tccagattta
     1981 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     2041 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     2101 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     2161 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     2221 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     2281 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     2341 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     2401 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     2461 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     2521 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     2581 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2641 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2701 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2761 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta aga
//
