LOCUS       sequence-236036-        4379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for Bxb1-mScarleti-LAA (Bxb1 
            integrase tagged with an mScarlet-I reporter and an LAA degradation 
            tag). Please see Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4379)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C67m
FEATURES             Location/Qualifiers
     source          1..4379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1914..1934
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1940..1957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2111..2699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2191..2210)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2870..3661,3662..3730))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3493..3512
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3731..3835)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3903..3921
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3925..3968)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3971..3992
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             join(4008..4379,1..1131)
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MVRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDL
                     DVSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKL
                     VVSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWG
                     YLPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQ
                     GREPQGREWSATALKRSMISGAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRA
                     ELVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGT
                     VAMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRA
                     GSPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRS
                     MNVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
ORIGIN
        1 gcgcttatgg gaacggtggc gcagatggaa ttagaagcga tcaaagagcg gaaccgttcg
       61 gctgcgcatt tcaatatccg cgccgggaaa taccgaggat ccctgccgcc gtggggatac
      121 ctgcctacgc gcgtggacgg ggagtggcgg ctggtgccgg accctgtgca gcgagagcgc
      181 atcctcgagg tgtatcaccg cgtcgtcgac aaccacgagc cgctgcatct ggtggcccac
      241 gacctgaacc ggcgtggtgt cctgtcgccg aaggactact tcgcgcagct gcaaggccgc
      301 gagccgcagg gccgggagtg gtcggctacc gcgctgaagc gatcgatgat ctccggggcg
      361 atgctcgggt acgcgactct gaacggtaag accgtccgag acgacgacgg agccccgctg
      421 gtgcgggctg agccgatcct gacccgtgag cagctggagg cgctgcgcgc cgagctcgtc
      481 aagacctccc gggcgaagcc cgcggtgtct accccgtcgc tgctgctgcg ggtgttgttc
      541 tgcgcggtgt gcggggagcc cgcgtacaag ttcgccgggg gaggacgtaa gcacccgcgc
      601 taccgctgcc gctcgatggg gttcccgaag cactgcggga acggcacggt ggcgatggcc
      661 gagtgggacg cgttctgcga ggagcaggta ctggatctgc tcggggacgc ggagcgtctg
      721 gagaaagtct gggtagcggg ctcggactcc gcggtcgaac tcgcggaggt gaacgcggag
      781 ctggtggacc tgacgtcgct gatcggctcc ccggcctacc gggcgggctc tccgcagcga
      841 gaagcactgg atgcccgtat tgcggcgctg gccgcgcggc aagaggagct ggagggcctg
      901 gaggctcgcc cgtctggctg ggagtggcgc gaaaccgggc agcggttcgg ggactggtgg
      961 cgggagcagg acaccgcggc aaagaacacc tggcttcggt cgatgaacgt tcggctgacg
     1021 ttcgacgtcc gcggcgggct gactcgcacg atcgacttcg gggatcttca ggagtacgag
     1081 cagcatctca ggctcggcag cgtggtcgaa cggctacaca ccgggatgtc gggtagcggc
     1141 agcggtagcg gtagtgggag cggcagcatg gtcagtaaag gcgaagcagt tatcaaagag
     1201 ttcatgcgct tcaaagttca tatggaaggg tcgatgaacg ggcacgaatt tgaaattgaa
     1261 ggcgaaggcg aaggccgccc atatgaaggg acccaaaccg caaagcttaa ggttactaaa
     1321 ggcggtccat tacccttttc gtgggacatt ttaagcccac agtttatgta cgggagtcgc
     1381 gctttcatca agcaccctgc ggacatccca gattactaca aacagtcttt ccccgagggg
     1441 ttcaagtggg agcgcgtgat gaacttcgag gatggcggag ccgtgacggt cacccaagat
     1501 acctctttgg aggacggtac gttgatctac aaagtgaaat tgcgtggcac gaattttcca
     1561 cctgatgggc ctgtcatgca gaaaaagaca atgggatggg aagcttccac ggagcgcctt
     1621 tacccagagg acggtgttct taaaggggat atcaaaatgg cgctgcgtct taaagatgga
     1681 ggccgctacc tggcggactt caagactact tacaaggcca aaaaaccagt gcagatgccg
     1741 ggtgcgtaca atgtagatcg taaattagat attacaagtc acaatgaaga ttacacggtc
     1801 gtagagcagt atgagcgcag tgaggggcgt cactctacgg gcggtatgga cgagttatac
     1861 aaggcggcta acgacgagaa ttatgcattg gccgcctaat aaaggtagag acctactagt
     1921 agcggccgct gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1981 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2041 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2101 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2161 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2221 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2281 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2341 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2401 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2461 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2521 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2581 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2641 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2701 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2761 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2821 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2881 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2941 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3001 tgataccgcg cgacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3061 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3121 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3181 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3241 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3301 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3361 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3421 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3481 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3541 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3601 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3661 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3721 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3781 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3841 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3901 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
     3961 atgatttctg gaattcgcgg ccgcttctag agactagtgg gtctcaaatg gtgagagccc
     4021 tggtagtcat ccgcctgtcc cgcgtcaccg atgctacgac ttcaccggag cgtcagctgg
     4081 agtcttgcca gcagctctgc gcccagcgcg gctgggacgt cgtcggggta gcggaggatc
     4141 tggacgtctc cggggcggtc gatccgttcg accggaagcg cagaccgaac ctggcccggt
     4201 ggctagcgtt cgaggagcaa ccgtttgacg tgatcgtggc gtaccgggta gatcggttga
     4261 cccgatcgat ccggcatctt cagcagctgg tccactgggc cgaggaccac aagaagctgg
     4321 tcgtctccgc gaccgaagcg cacttcgata cgacgacgcc gtttgcggcg gtcgtcatc
//
