LOCUS       sequence-236044-       10649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Luo JH, Fu ZY, Losi G, Kim BG, Prybylowski K, Vissel B, Vicini S
  TITLE     Functional expression of distinct NMDA channel subunits tagged with 
            green fluorescent protein in hippocampal neurons in culture.
  JOURNAL   Neuropharmacology. 2002 Mar . 42(3):306-18.
  PUBMED    11897109
REFERENCE   2  (bases 1 to 10649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuropharmacology. 2002 Mar . 42(3):306-18."
COMMENT     Sequence Label: pEGFP-NR2B
FEATURES             Location/Qualifiers
     source          1..10649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             join(1377..1379,1380..1382,1383..2093)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLSMDELYK"
     primer_bind     complement(1422..1443)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1683..1702)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2030..2051
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      6949..6958
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6962..7096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6999..7018)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7053..7072
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        7165..7522
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7165..7356
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7188..7208)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7373..7508
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7542..7559)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7542..7558)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7551..7573)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7771..8226
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7858..7877)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8068..8089
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8243..8262)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8362..8384
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8422..8440)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8508..8612
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8613..8681,8682..9473)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8831..8850)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9644..10232
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10133..10152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10386..10403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10520..10541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10556..10561,10562..10579,10580..10586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10594..10610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10599..10621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10618..10634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10618..10634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcggcacg aggctgaaga ctccttaaat atatatatat
      961 atatatattc gggctactaa cctcacatgc acatgggata atgactctgg attctgcatt
     1021 gtgagctgct ctccacaccc tgagatcccc tcttacatta cattttttcc tttgaatttg
     1081 catctcgtca agacacaaga ttaaaaccaa atttacacta cactggattt taaattttct
     1141 tccgttcctt tatcctccgt ctttcttatg tggatatgca agcgagaaga ggaccctgga
     1201 tattcccaac atgctctctc ccttaatctg tccgcctaga ggtttggcgt ctacaaacca
     1261 agagagccga ctagctgaag atgaagccca gcgcagagtg ctgttccccc aagttctggt
     1321 tggtgttggc cgtcttggcc gtatcaggca gcaaagctcg ttcccaaaag ggccccatgg
     1381 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1441 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1501 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1561 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     1621 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1681 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1741 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1801 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     1861 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1921 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1981 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     2041 tggagttcgt gaccgccgcc gggatcactc tcagcatgga cgagctgtac aagggccccc
     2101 ccagcattgg catcgctgtc atcctcgtgg gcacttcaga cgaagtggcc ataaaagacg
     2161 cccacgagaa agatgacttc catcatctct cagtagttcc ccgggtggag ctggtagcca
     2221 tgaacgaaac tgacccaaag agcatcatca cccgtatctg cgatcttatg tctgaccgga
     2281 agatccaggg ggtggtgttc gcggatgaca ccgaccaaga agccatcgct cagatcctcg
     2341 acttcatttc tgctcagact ctcaccccca tcctgggcat ccatgggggc tcatctatga
     2401 taatggcgga taaggatgag tcctccatgt tcttccagtt tggcccgtct atcgaacagc
     2461 aagcttccgt catgctcaac atcatggaag aatatgactg gtacatcttt tccatcgtca
     2521 ccacctactt ccctggctac caggactttg tgaacaagat ccgcagtacc atcgagaaca
     2581 gcttcgtggg ctgggagctc gaggaagtcc tcctgctaga catgtctctg gacgatggcg
     2641 actctaagat tcagaatcag ctgaagaagc tccaaagccc catcattctc ctttattgca
     2701 cgaaggagga agccacctac atttttgaag tagctaactc agttgggctg actggctacg
     2761 gctacacgtg gattgtgccg agtctggtgg ccggggatac ggacacggtg ccttcagagt
     2821 tccccacggg gcttatctct gtgtcttatg atgaatggga ctatggcctt cctgccagag
     2881 tgagagatgg aattgccatc atcaccactg ctgcctcgga catgctgtcc gaacacagtt
     2941 tcatccctga gcccaagagc agttgctaca acacccacga gaagaggatc taccagtcta
     3001 acatgttgaa taggtatctg atcaatgtca cttttgaagg gagaaacctg tccttcagcg
     3061 aagatggcta ccagatgcat ccgaagctgg tgataatcct tctgaacaag gagaggaagt
     3121 gggagagggt ggggaaatgg aaggacaagt ccctgcagat gaagtattat gtgtggcctc
     3181 ggatgtgtcc tgagactgag gagcaagagg atgaccatct gagcattgtc accttggagg
     3241 aggcgccatt tgtcattgtg gaaagcgtgg accctctcag tggaacctgc atgaggaata
     3301 cagtcccgtg ccagaagcgc atcatctctg agaataaaac agatgaggaa ccaggctaca
     3361 tcaaaaaatg ctgcaagggg ttctgtattg acatccttaa gaaaatttct aagtctgtga
     3421 agttcaccta tgacctttac ctggtgacca atggcaagca cgggaagaag attaatggga
     3481 cctggaatgg catgatcggt gaggtggtca tgaagagggc ctacatggca gtgggatcac
     3541 taactatcaa tgaagaacgg tcagaggtgg ttgacttctc tgtacccttc atagaaactg
     3601 gcatcagtgt catggtatct cgcagcaatg ggactgtgtc accttctgcc ttcttagagc
     3661 cattcagcgc tgacgtgtgg gtgatgatgt ttgtgatgct gctcattgtt tctgcggtgg
     3721 ctgtctttgt ctttgaatac ttcagccctg tgggttacaa caggtgccta gccgatggca
     3781 gagagccagg aggcccatct ttcaccatcg gcaaagcaat ttggttactc tggggtctgg
     3841 tgtttaacaa ctccgtacct gtgcagaacc caaaggggac cacctccaag atcatggtgt
     3901 cagtgtgggc cttctttgct gtcattttcc tggccagcta cactgccaac ttagcagcct
     3961 tcatgatcca agaggagtat gtggaccagg tttctggcct gagtgacaag aagttccaga
     4021 gacctaatga cttctcaccc cctttccgct ttgggactgt gcccaatggc agcacagaga
     4081 ggaatatccg taataactat gcagaaatgc atgcctacat gggaaagttc aaccaaaggg
     4141 gtgtagatga tgcattgctc tccctgaaaa cagggaagct tgatgcattc atctatgatg
     4201 cagctgtgct caactacatg gctggaaggg acgaaggctg caaactggtg accattggca
     4261 gtggcaaggt ctttgcttct accggctatg gcattgctat ccaaaaggac tccgggtgga
     4321 agcgccaggt ggacctggct atcctgcagc tgtttggaga tggggagatg gaagaactgg
     4381 aagctctctg gctcactggc atttgccaca atgagaagaa tgaggtgatg agcagccagc
     4441 tggacatcga caatatggca ggtgtcttct atatgttggg ggcagccatg gccctcagcc
     4501 tcatcacctt catctgtgag catctgttct attggcagtt ccggcattgc ttcatgggtg
     4561 tctgttctgg caagcctggc atggtcttct ccatcagcag aggtatctac agctgtatcc
     4621 atggggtagc catagaggag cgccaatccg tgatgaactc ccccactgcc accatgaaca
     4681 acacccactc caacatccta cgcttgctcc gcacggccaa gaacatggcc aacctgtctg
     4741 gagtaaacgg ctcccctcag agtgccctgg acttcatccg ccgagagtcc tccgtctacg
     4801 acatctctga gcatcgtcgc agcttcacgc attcagactg caagtcttac aataacccac
     4861 cctgtgagga aaacctgttc agtgactaca ttagcgaggt agagagaaca tttggtaacc
     4921 tgcagctgaa ggacagcaat gtgtaccaag accactatca ccatcaccac cggccacaca
     4981 gcatcggcag caccagctcc attgatgggc tctatgactg tgacaaccca cccttcacca
     5041 cccagcccag gtcaatcagc aagaaacccc tggacatcgg cctgccctcc tccaaacata
     5101 gccagctcag cgacctgtat ggcaagttct ctttcaagag tgaccgctac agtggccacg
     5161 acgacttgat tcgatcggat gtctccgaca tctccacgca cactgtcacc tatgggaaca
     5221 tcgagggcaa cgcagccaag aggaggaaac agcagtataa ggacagtcta aagaagcggc
     5281 cagcctcggc caaatcgagg agggagtttg atgaaatcga gctggcctac cgtcgccgac
     5341 caccccgctc cccggaccac aagcgctact tcagggacaa agaagggctc cgagacttct
     5401 acctggacca gttccgaaca aaggagaact cgcctcactg ggagcacgtg gacttgactg
     5461 acatttacaa agaacgcagt gacgacttca agcgagattc ggtcagtgga ggtgggccct
     5521 gtaccaacag gtctcacctc aaacacggaa cgggcgagaa gcacggagtg gtaggcgggg
     5581 tgcctgctcc ttgggagaag aacctgacca atgtggattg ggaggaccgg tctgggggca
     5641 acttctgccg cagctgtcct tccaagctgc acaattactc ctcgacggtg gcagggcaga
     5701 actcgggccg gcaggcctgc atcagatgtg aggcctgtaa gaaggctggt aacctgtacg
     5761 acatcagcaa ggacaactcc ctgcaggaac tggaccagcc ggctgccccc gtggctgtga
     5821 catccaacgc ctccagcacc aagtaccctc aaagcccgac taattccaag gctcagaaga
     5881 agaatcggaa caaactgcgc cggcagcatt cctacgacac cttcgtggac ctgcagaagg
     5941 aggaggccgc cttggcccca cgcagcgtga gcctgaaaga caagggccga ttcatggatg
     6001 ggagccccta cgcccatatg tttgagatgc cagctggtga gagctccttt gccaacaagt
     6061 cctcagtgcc cactgccgga caccaccaca acaaccccgg cagcggctac atgctcagca
     6121 agtcgctcta ccctgaccgg gtcacgcaaa accctttcat ccccactttt ggggatgacc
     6181 agtgcttgct tcacggcagc aaatcctact tcttcaggca gcccacggtg gcaggggcgt
     6241 caaaaacaag gccggacttc cgggcccttg tcaccaataa gccagtggtg tcagcccttc
     6301 atggggctgt gccaggtcgt ttccagaagg acatttgtat agggaaccag tccaacccct
     6361 gtgtgcctaa caacaaaaac cccagggctt tcaatggctc cagcaatgga catgtttatg
     6421 agaaactttc tagtattgag tctgatgtct gagtgaggga agagagagag gttaaggtgg
     6481 gtacgggagg gatagggctg tgggccgcgt ggtgcgcatg tcatggaaag agtcgggggt
     6541 gaacttggct cccattcgct ttttcttctt cttttaattt ctctatggga tcctggagtt
     6601 ctggttcctt actgaaggca accctcgtgg ccagcaccat ttctcctccc tcgcgcagtt
     6661 ctctccttcc cgcatctgtc caccattcct gtttccatga gagaatagaa cggggcctca
     6721 gtgtgggagg atggagagga gaccaaagcg ctgcctgtgt gcttctttct agcgcagaag
     6781 ggctcttagc agttcacttt gagcgaggct ttcctgatgc tgctctcttt gttcaggtga
     6841 ggaagcgaag gtgttctgag gaaggccatt gaacctcctg ttcataagag agaagaggct
     6901 ttcactgaat tccccgggga tcctctagag tcgacctgca gaagcttggc cgccatggcc
     6961 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7021 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7081 ttatcatgtc tggatcggga attaattcgg cgcagcacca tggcctgaaa taacctctga
     7141 aagaggaact tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca
     7201 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7261 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7321 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7381 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7441 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7501 tggaggccta ggcttttgca aaaagctgtt aacagcttgg cactggccgt cgttttacaa
     7561 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     7621 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     7681 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     7741 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     7801 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     7861 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     7921 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     7981 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     8041 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     8101 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     8161 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     8221 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     8281 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     8341 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     8401 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     8461 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     8521 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     8581 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     8641 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8701 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8761 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     8821 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     8881 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     8941 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     9001 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     9061 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9121 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     9181 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     9241 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9301 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9361 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9421 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     9481 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9541 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9601 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9661 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9721 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9781 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9841 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9901 catgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9961 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10021 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10081 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10141 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10201 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10261 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    10321 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    10381 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    10441 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    10501 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    10561 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    10621 gaaacagcta tgacatgatt acgaattaa
//
