LOCUS       sequence-236045-       12598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of catalytic inactive form of full-length TET1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12598)
  AUTHORS   Vincenzetti L, Leoni C, Chirichella M, Kwee I, Monticelli S
  TITLE     The contribution of active and passive mechanisms of 5mC and 5hmC 
            removal in human T lymphocytes is differentiation- and 
            activation-dependent.
  JOURNAL   Eur J Immunol. 2019 Jan 30. doi: 10.1002/eji.201847967.
  PUBMED    30698829
REFERENCE   2  (bases 1 to 12598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Immunol. 2019 Jan 30. doi: 10.1002/eji.201847967."
COMMENT     Sequence Label: pEF1a_FL MUT TET1
FEATURES             Location/Qualifiers
     source          1..12598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..64)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        352..1530
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          583..1521
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1478..1498
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1547..1566
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1547..1565
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1598..1607
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1607..1630
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1643..1669
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             1643..1669
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8108..8149
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8159..8176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8199..8216)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8205..8429
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8475..8903
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8562..8581)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8772..8793
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8912..8932)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8917..9246
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9097..9232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9159..9178
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9313..10107
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9367..9386)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9977..9996
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10283..10404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10320..10339)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10374..10393
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10453..10469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10453..10469)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10466..10488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10477..10493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10501..10507,10508..10525,10526..10531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10546..10567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10684..10701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10844..11432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10924..10943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11603..12394,12395..12463))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12226..12245
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12464..12568)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctaggctttt gcaaaaagct ttgcaaagat ggataaagtt ttaaacagag
      301 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
      361 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
      421 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
      481 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
      541 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
      601 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
      661 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
      721 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
      781 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
      841 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
      901 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
      961 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     1021 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     1081 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     1141 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     1201 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     1261 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     1321 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     1381 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     1441 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     1501 agtttttttc ttccatttca ggtgtcgtga ggaattagct tggtactaat acgactcact
     1561 atagggagac ccaagctggc taggtaagct tggtaccgcc accatggact acaaggacga
     1621 cgatgacaag ctcgatggag gataccccta cgacgtgccc gactacgccg gaggactcga
     1681 ctctcgatcc cgccatgcaa ggccttccag attagtcagg aaggaagatg taaacaaaaa
     1741 aaagaaaaac agccaactac gaaagacaac caagggagcc aacaaaaatg tggcatcagt
     1801 caagacttta agccctggaa aattaaagca attaattcaa gaaagagatg ttaagaaaaa
     1861 aacagaacct aaaccacccg tgccagtcag aagccttctg acaagagctg gagcagcacg
     1921 catgaatttg gataggactg aggttctttt tcagaaccca gagtccttaa cctgcaatgg
     1981 gtttacaatg gcgctacgaa gcacctctct tagcaggcga ctctcccaac ccccactggt
     2041 cgtagccaaa tccaaaaagg ttccactttc taagggttta gaaaagcaac atgattgtga
     2101 ttataagata ctccctgctt tgggagtaaa gcactcagaa aatgattcgg ttccaatgca
     2161 agacacccaa gtccttcctg atatagagac tctaattggt gtacaaaatc cctctttact
     2221 taaaggtaag agccaagaga caactcagtt ttggtcccaa agagttgagg attccaagat
     2281 caatatccct acccacagtg gccctgcagc tgagatcctt cctgggccac tggaagggac
     2341 acgctgtggt gaaggactat tctctgaaga gacattgaat gataccagtg gttccccaaa
     2401 aatgtttgct caggacacag tgtgtgctcc ttttccccaa agagcaaccc ccaaagttac
     2461 ctctcaagga aaccccagca ttcagttaga agagttgggt tcacgagtag aatctcttaa
     2521 gttatctgat tcttacctgg atcccattaa aagtgaacat gattgctacc ccacctccag
     2581 tcttaataag gttatacctg acttgaacct tagaaactgc ttggctcttg gtgggtctac
     2641 gtctcctacc tctgtaataa aattcctctt ggcaggctca aaacaagcga cccttggtgc
     2701 taaaccagat catcaagagg ccttcgaagc tactgcaaat caacaggaag tttctgatac
     2761 cacctctttc ctaggacagg cctttggtgc tatcccacat caatgggaac ttcctggtgc
     2821 tgacccagtt catggtgagg ccctgggtga gaccccagat ctaccagaga ttcctggtgc
     2881 tattccagtc caaggagagg tctttggtac tattttagac caacaagaaa ctcttggtat
     2941 gagtgggagt gttgtcccag acttgcctgt cttccttcct gttcctccaa atccaattgc
     3001 tacctttaat gctccttcca aatggcctga gccccaaagc actgtctcat atggacttgc
     3061 agtccagggt gctatacaga ttttgccttt gggctcagga cacactcctc aatcatcatc
     3121 aaactcagag aaaaattcat tacctccagt aatggctata agcaatgtag aaaatgagaa
     3181 gcaggttcat ataagcttcc tgccagctaa cactcagggg ttcccattag cccctgagag
     3241 aggactcttc catgcttcac tgggtatagc ccaactctct caggctggtc ctagcaaatc
     3301 agacagaggg agctcccagg tcagtgtaac cagcacagtt catgttgtca acaccacagt
     3361 ggtgactatg ccagtgccaa tggtcagtac ctcctcttct tcctatacca ctttgctacc
     3421 gactttggaa aagaagaaaa gaaagcgatg tggggtctgt gaaccctgcc agcagaagac
     3481 caactgtggt gaatgcactt actgcaagaa cagaaagaac agccatcaga tctgtaagaa
     3541 aagaaaatgt gaggagctga aaaagaaacc atctgttgtt gtgcctctgg aggttataaa
     3601 ggaaaacaag aggccccaga gggaaaagaa gcccaaagtt ttaaaggcag attttgacaa
     3661 caaaccagta aatggcccca agtcagaatc catggactac agtagatgtg gtcatgggga
     3721 agaacaaaaa ttggaattga acccacatac tgttgaaaat gtaactaaaa atgaagacag
     3781 catgacaggc atcgaggtgg agaagtggac acaaaacaag aaatcacagt taactgatca
     3841 cgtgaaagga gattttagtg ctaatgtccc agaagctgaa aaatcgaaaa actctgaagt
     3901 tgacaagaaa cgaaccaaat ctccaaaatt gtttgtacaa accgtaagaa atggcattaa
     3961 acatgtacac tgtttaccag ctgaaacaaa tgtttcattt aaaaaattca atattgaaga
     4021 attcggcaag acattggaaa acaattctta taaattccta aaagacactg caaaccataa
     4081 aaacgctatg agctctgttg ctactgatat gagttgtgat catctcaagg ggagaagtaa
     4141 cgttttagta ttccagcagc ctggctttaa ctgcagttcc attccacatt cttcacactc
     4201 catcataaat catcatgcta gtatacacaa tgaaggtgat caaccaaaaa ctcctgagaa
     4261 tataccaagt aaagaaccaa aagatggatc tcccgttcaa ccaagtctct tatcgttaat
     4321 gaaagatagg agattaacat tggagcaagt ggtagccata gaggccctga ctcaactctc
     4381 agaagcccca tcagagaatt cctccccatc aaagtcagag aaggatgagg aatcagagca
     4441 gagaacagcc agtttgctta atagctgcaa agctatcctc tacactgtaa gaaaagacct
     4501 ccaagaccca aacttacagg gagagccacc aaaacttaat cactgtccat ctttggaaaa
     4561 acaaagttca tgcaacacgg tggttttcaa tgggcaaact actacccttt ccaactcaca
     4621 tatcaactca gctactaacc aagcatccac aaagtcacat gaatattcaa aagtcacaaa
     4681 ttcattatct ctttttatac caaaatcaaa ttcatccaag attgacacca ataaaagtat
     4741 tgctcaaggg ataattactc ttgacaattg ttccaatgat ttgcatcagt tgccaccaag
     4801 aaataatgaa gtggagtatt gcaaccagtt actggacagc agcaaaaaat tggactcaga
     4861 tgatctatca tgtcaggatg caacccatac ccaaattgag gaagatgttg caacacagtt
     4921 gacacaactt gcttcgataa ttaagatcaa ttatataaaa ccagaggaca aaaaagttga
     4981 aagtacacca acaagccttg tcacatgtaa tgtacagcaa aaatacaatc aggagaaggg
     5041 cacaatgcaa cagaaaccac cttcaagtgt acacaataat catggttcat cattaacaaa
     5101 acaaaagaac ccaacccaga aaaagacaaa atccacccca tcaagagatc ggcggaaaaa
     5161 gaagcccaca gttgtaagtt atcaagaaaa tgatcggcag aagtgggaaa agttgtccta
     5221 tatgtatggc acaatatgcg acatttggat agcatcgaaa tttcaaaatt ttgggcaatt
     5281 ttgtccacat gattttccta ctgtatttgg gaaaatttct tcctcgacca aaatatggaa
     5341 accactggct caaacgaggt ccattatgca acccaaaaca gtatttccac cactcactca
     5401 gataaaatta cagagatatc ctgaatcagc agaggaaaag gtgaaggttg aaccattgga
     5461 ttcactcagc ttatttcatc ttaaaacgga atccaacggg aaggcattca ctgataaagc
     5521 ttataattct caggtacagt taacggtgaa tgccaatcag aaagcccatc ctttgaccca
     5581 gccctcctct ccacctaacc agtgtgctaa cgtgatggca ggcgatgacc aaatacggtt
     5641 tcagcaggtt gttaaggagc aactcatgca tcagagactg ccaacattgc ctggtatctc
     5701 tcatgaaaca cccttaccgg agtcagcact aactctcagg aatgtaaatg tagtgtgttc
     5761 aggtggaatt acagtggttt ctaccaaaag tgaagaggaa gtctgttcat ccagttttgg
     5821 aacatcagaa ttttccacag tggacagtgc acagaaaaat tttaatgatt atgccatgaa
     5881 cttctttact aaccctacaa aaaacctagt gtctataact aaagattctg aactgcccac
     5941 ctgcagctgt cttgatcgag ttatacaaaa agacaaaggc ccatattata cacaccttgg
     6001 ggcaggacca agtgttgctg ctgtcaggga aatcatggag aataggtatg gtcaaaaagg
     6061 aaacgcaata aggatagaaa tagtagtgta caccggtaaa gaagggaaaa gctctcatgg
     6121 gtgtccaatt gctaagtggg ttttaagaag aagcagtgat gaagaaaaag ttctttgttt
     6181 ggtccggcag cgtacaggcc accactgtcc aactgctgtg atggtggtgc tcatcatggt
     6241 gtgggatggc atccctcttc caatggccga ccggctatac acagagctca cagagaatct
     6301 aaagtcatac aatgggcacc ctaccgacag aagatgcacc ctcaatgaaa atcgtacctg
     6361 tacatgtcaa ggaattgatc cagagacttg tggagcttca ttctcttttg gctgttcatg
     6421 gagtatgtac tttaatggct gtaagtttgg tagaagccca agccccagaa gatttagaat
     6481 tgatccaagc tctcccttac atgaaaaaaa ccttgaagat aacttacaga gtttggctac
     6541 acgattagct ccaatttata agcagtatgc tccagtagct taccaaaatc aggtggaata
     6601 tgaaaatgtt gcccgagaat gtcggcttgg cagcaaggaa ggtcgtccct tctctggggt
     6661 cactgcttgc ctggacttct gtgctcatcc ctacagggct attcacaaca tgaataatgg
     6721 aagcactgtg gtttgtacct taactcgaga agataaccgc tctttgggtg ttattcctca
     6781 agatgagcag ctccatgtgc tacctcttta taagctttca gacacagatg agtttggctc
     6841 caaggaagga atggaagcca agatcaaatc tggggccatc gaggtcctgg caccccgccg
     6901 caaaaaaaga acgtgtttca ctcagcctgt tccccgttct ggaaagaaga gggctgcgat
     6961 gatgacagag gttcttgcac ataagataag ggcagtggaa aagaaaccta ttccccgaat
     7021 caagcggaag aataactcaa caacaacaaa caacagtaag ccttcgtcac tgccaacctt
     7081 agggagtaac actgagaccg tgcaacctga agtaaaaagt gaaaccgaac cccattttat
     7141 cttaaaaagt tcagacaaca ctaaaactta ttcgctgatg ccatccgctc ctcacccagt
     7201 gaaagaggca tctccaggct tctcctggtc cccgaagact gcttcagcca caccagctcc
     7261 actgaagaat gacgcaacag cctcatgcgg gttttcagaa agaagcagca ctccccactg
     7321 tacgatgcct tcgggaagac tcagtggtgc caatgcagct gctgctgatg gccctggcat
     7381 ttcacagctt ggcgaagtgg ctcctctccc caccctgtct gctcctgtga tggagcccct
     7441 cattaattct gagccttcca ctggtgtgac tgagccgcta acgcctcatc agccaaacca
     7501 ccagccctcc ttcctcacct ctcctcaaga ccttgcctct tctccaatgg aagaagatga
     7561 gcagcattct gaagcagatg agcctccatc agacgaaccc ctatctgatg accccctgtc
     7621 acctgctgag gagaaattgc cccacattga tgagtattgg tcagacagtg agcacatctt
     7681 tttggatgca aatattggtg gggtggccat cgcacctgct cacggctcgg ttttgattga
     7741 gtgtgcccgg cgagagctgc acgctaccac tcctgttgag caccccaacc gtaatcatcc
     7801 aacccgcctc tcccttgtct tttaccagca caaaaaccta aataagcccc aacatggttt
     7861 tgaactaaac aagattaagt ttgaggctaa agaagctaag aataagaaaa tgaaggcctc
     7921 agagcaaaaa gaccaggcag ctaatgaagg tccagaacag tcctctgaag taaatgaatt
     7981 gaaccaaatt ccttctcata aagcattaac attaacccat gacaatgttg tcaccgtgtc
     8041 cccttatgct ctcacacacg ttgcggggcc ctataaccat tgggtctgat ctagagggcc
     8101 cttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca
     8161 tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     8221 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     8281 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     8341 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     8401 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     8461 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     8521 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     8581 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     8641 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     8701 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     8761 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     8821 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     8881 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     8941 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9001 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9061 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9121 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9181 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9241 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     9301 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     9361 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     9421 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     9481 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     9541 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     9601 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     9661 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     9721 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     9781 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     9841 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     9901 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     9961 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
    10021 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
    10081 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca
    10141 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
    10201 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    10261 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
    10321 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10381 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
    10441 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    10501 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    10561 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    10621 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    10681 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    10741 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    10801 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10861 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10921 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10981 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    11041 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    11101 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    11161 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    11221 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    11281 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    11341 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    11401 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    11461 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    11521 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    11581 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    11641 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    11701 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    11761 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    11821 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    11881 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    11941 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    12001 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    12061 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    12121 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    12181 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    12241 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    12301 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    12361 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    12421 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    12481 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    12541 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgt
//
