LOCUS       sequence-236047-        2824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for LasR-LSTPLVMI (Adam Meyer's 
            improved LasR activator, selected for dynamic range and low 
            cross-sensitivity with methods from 
            https://doi.org/10.1101/285866)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2824)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C73m
FEATURES             Location/Qualifiers
     source          1..2824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..66
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(70..113)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    116..137
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    883..903
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(909..926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1080..1668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1160..1179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1839..2630,2631..2699))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2462..2481
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2700..2804)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
       61 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt
      121 cgcggccgct tctagagact agtgggtctc aaatggcctt ggttgacggt tttcttgagc
      181 tggaacgctc aagtggaaaa ttggagttga gcgccatcct gcagaagatg gcgagcgacc
      241 tcggattctc gaagatcctg ttcggcctgt tgcctaagga cagccaggac tacgagaacg
      301 ccttcatcgt cagcaactac ccggccgcct ggcgcgagca ttacgaccga actggctacg
      361 cgcgggtcga cccgacggtc agtcactgta cccagagcgt actgccgatt ttctgggaac
      421 cgcccatcta ccagacgcga aagcagcacg agttcttcga ggaagccttg gccgctggcc
      481 tggtgtatgg actgaccatg ccgctgcatg gtgctcgcgg cgaactcggc gcgctgagcc
      541 tcagcgtgga agcggaaaac cgggccgagg ccaaccgttt catggagtcg gtcctgccga
      601 ccctgtggat gctcaaggac tacgcactgc agagcggtgt cggaatggcc ttcgaacatc
      661 cgatcagcaa accggtggtt ctgaccagcc gagagaagga agtgttacag tggtgcgcca
      721 tcggcaagac cagctgggag atatcggtta tctgcaactg ctcggaagcc aatgtgaact
      781 tccatatggg aaatattcgg cggaagttcg gtgtgacctc ccgccgcgta gcggccatta
      841 tggccgttaa tttgggtctt attactctct gaggtagaga cctactagta gcggccgctg
      901 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      961 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1021 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1081 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1141 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1201 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1261 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1321 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1381 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1441 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1501 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1561 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1621 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1681 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1741 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1801 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     1861 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     1921 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcgc
     1981 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2041 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2101 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     2161 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     2221 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     2281 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     2341 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     2401 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     2461 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     2521 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     2581 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     2641 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     2701 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     2761 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     2821 gtgc
//
