LOCUS       sequence-236049-        3007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for NahRQ-VHAC (Adam Meyer's 
            improved NahR activator from https://doi.org/10.1101/285866, 
            selected for dynamic range and low cross-sensitivity)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3007)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C75m
FEATURES             Location/Qualifiers
     source          1..3007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     11..29
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(33..76)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    79..100
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    1029..1049
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1055..1072)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1226..1814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1306..1325)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1985..2776,2777..2845))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2608..2627
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2846..2950)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
       61 cgctaaggat gatttctgga attcgcggcc gcttctagag actagtgggt ctcaaatgga
      121 actgcgtgac ctggatttaa acctgctggt ggtgttcaac cagttgctgg tcgacagacg
      181 cgtctctgtc actgcggaga acctgggcct gacccagcct gccgtgagca atgcgctgaa
      241 acgcctgcgc acctcgctac aggacccact cttcgtgcgc acacatcagg gaatggaacc
      301 cacaccctat gccgcgcatc tggccgagca cgtcacttcg gccatgcacg cactgcgcaa
      361 cgccctacag caccatgaaa gcttcgatcc gctgaccagc gagcgtacct tcaccctggc
      421 catgaccgac attggcgaga tctacttcat gccgcggctg atggatgcgc tggctcacca
      481 ggcccccaat tgcgtgatca gtacggtgcg cgacagttcg atgagcctga tgcaggcctt
      541 gcagaacgga accgtggact tggccgtggg cctgcttccc aatctgcaaa ctggcttctt
      601 tcagcgccgg ctgctccaga atcactacgt gtgcctatgt cgcaaggacc atccagtcac
      661 ccgcgaaccc ctgactctgg agcgcttctg ttcctacggc cacgtgcgtg tcatcgccgc
      721 tggcaccggc cacggcgagg tggacacgta catgacacgg gtcggcatcc ggcgcgacat
      781 ccgtctggaa gtgccgcact tcgccgccgt tggccacatc ctccagcgca ccgatctgct
      841 cgccactgtg ccgatatgtt tagccgactg ctgcgtagag cccttcggcc taagcgcctt
      901 gccgcaccca gtcgtcttgc ctgaaatagc catcaacatg ttctggcatg cgaagtacca
      961 caaggaccta gccaatattt ggttgcggca actgatgttt gacctgttta cggattgagg
     1021 tagagaccta ctagtagcgg ccgctgcagg cttcctcgct cactgactcg ctgcgctcgg
     1081 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1141 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1201 gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca
     1261 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1321 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1381 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1441 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1501 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1561 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1621 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1681 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1741 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1801 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1861 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1921 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1981 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2041 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2101 gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat ttatcagcaa
     2161 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2221 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2281 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2341 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2401 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2461 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2521 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2581 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2641 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2701 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2761 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2821 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2881 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2941 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     3001 tgacatt
//
