LOCUS       sequence-236053-        2924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for KanR (kanamycin resistance 
            protein). Please see Supplemental Documents for annotated Genbank 
            file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2924)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C76m
FEATURES             Location/Qualifiers
     source          1..2924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(55..846,847..915))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     678..697
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(916..1020)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1088..1106
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1110..1153)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1156..1177
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             1196..2011
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1269..1288)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    2023..2043
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(2049..2066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2220..2808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2300..2319)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
       61 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
      121 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
      181 tgcaatgata ccgcgcgacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
      241 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
      301 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
      361 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
      421 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
      481 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
      541 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
      601 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
      661 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
      721 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
      781 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
      841 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
      901 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
      961 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     1021 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     1081 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
     1141 taaggatgat ttctggaatt cgcggccgct tctagagact agtgggtctc aaatgatgag
     1201 ccatattcaa cgggaaacgt cttgctcccg tccgcgctta aactccaaca tggacgctga
     1261 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     1321 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     1381 caatgatgtt acagatgaga tggtccgtct caactggctg acggagttta tgcctctccc
     1441 gaccatcaag cattttatcc gtactcctga tgatgcgtgg ttactcacca ccgcgattcc
     1501 tgggaaaaca gccttccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     1561 tgcgctggcc gtgttcctgc gccggttaca ttcgattcct gtttgtaatt gtccttttaa
     1621 cagcgatcgt gtatttcgtc ttgctcaggc gcaatcacgc atgaataacg gtttggttga
     1681 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     1741 gcacaagctc ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     1801 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gggtcggaat
     1861 cgcagaccgt taccaggacc ttgccattct ttggaactgc ctcggtgagt tttctccttc
     1921 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     1981 gtttcatttg atgctcgatg agtttttcta aaggtagaga cctactagta gcggccgctg
     2041 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2101 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2161 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2221 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2281 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2341 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2401 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2461 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2521 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2581 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2641 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2701 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2761 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2821 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2881 atgagattat caaaaaggat cttcacctag atccttttaa atta
//
