LOCUS       sequence-236054-       16821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon for dox-inducible expression of the OKMS (Oct4, 
            Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette 
            (with mCherry). SpCas9 is constitutively expressed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16821)
  AUTHORS   Kagawa H, Shimamoto R, Kim SI, Oceguera-Yanez F, Yamamoto T, 
            Schroeder T, Woltjen K
  TITLE     OVOL1 Influences the Determination and Expansion of iPSC 
            Reprogramming Intermediates.
  JOURNAL   Stem Cell Reports. 2018 Dec 28. pii: S2213-6711(18)30527-7. doi: 
            10.1016/j.stemcr.2018.12.008.
  PUBMED    30639212
REFERENCE   2  (bases 1 to 16821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2018 Dec 28. pii: S2213-6711(18)30527-7. doi: 
            10.1016/j.stemcr.2018.12.008."
COMMENT     Sequence Label: PB-TAC-OKMS-Cas9
FEATURES             Location/Qualifiers
     source          1..16821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   706..768
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1140..1158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1177..1195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1214..1232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1251..1269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1288..1306
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1325..1343
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1362..1380
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1399..1417
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1439..1463
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1543..1567
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2720..2785
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4226..4285
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5681..5746
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     protein_bind    complement(6786..6810)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(6852..7426,7427..7429,7430..7438)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7019..7036)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7246..7265
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7439..8149
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7584..7602)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7852..7871
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8161..8180)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8226..8281
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8280..8299)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        8718..9003
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          9283..9510
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      9522..9531
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9531..9596
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             9603..9623
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9648..13748
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9648..13748
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             13749..13796
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(13824..13841)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13830..14037
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   14448..14482
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(14631..14649)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14670..14686)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14670..14686)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14683..14705)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14694..14710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14718..14724,14725..14742,14743..14748))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14763..14784
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14901..14918)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15072..15660)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15152..15171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15831..16622,16623..16691))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16454..16473
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16692..16796)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctcggtattc
      661 acgacagcag gctgaataat aaaaaaatta gaaactatta tttaacccta gaaagataat
      721 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      781 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      841 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      901 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      961 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
     1021 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
     1081 cacatatcaa tattatgctc tcgttaatta actcgaggga tccaagctgg ccgcacgtct
     1141 ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat agagaaggta
     1201 cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc tccctatcag
     1261 tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc gacacgtctc
     1321 gagctcccta tcagtgatag agaaggtacg tctagaacgt ctccctatca gtgatagaga
     1381 agtcgacacg tctcgagctc cctatcagtg atagagaagg taccccctat ataagcagag
     1441 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     1501 gaagacaccg ggaccgatcc agcctccgcg gtggcggcca tcacaagttt gtacaaaaaa
     1561 gcaggctggc gccggaacca attcagtcga ctggatccgg taccgaattc gcccttaaaa
     1621 agcaggctcc accttcccca tggctggaca cctggcttca gacttcgcct tctcaccccc
     1681 accaggtggg ggtgatgggt cagcagggct ggagccgggc tgggtggatt ctcgaacctg
     1741 gctaagcttc caagggcctc caggtgggcc tggaatcgga ccaggctcag aggtattggg
     1801 gatctcccca tgtccgcccg catacgagtt ctgcggaggg atggcatact gtggacctca
     1861 ggttggactg ggcctagtcc cccaagttgg cgtggagact ttgcagcctg agggccaggc
     1921 aggagcacga gtggaaagca actcagaggg aacctcctct gagccctgtg ccgaccgccc
     1981 caatgccgtg aagttggaga aggtggaacc aactcccgag gagtcccagg acatgaaagc
     2041 cctgcagaag gagctagaac agtttgccaa gctgctgaag cagaagagga tcaccttggg
     2101 gtacacccag gccgacgtgg ggctcaccct gggcgttctc tttggaaagg tgttcagcca
     2161 gaccaccatc tgtcgcttcg aggccttgca gctcagcctt aagaacatgt gtaagctgcg
     2221 gcccctgctg gagaagtggg tggaggaagc cgacaacaat gagaaccttc aggagatatg
     2281 caaatcggag accctggtgc aggcccggaa gagaaagcga actagcattg agaaccgtgt
     2341 gaggtggagt ctggagacca tgtttctgaa gtgcccgaag ccctccctac agcagatcac
     2401 tcacatcgcc aatcagcttg ggctagagaa ggatgtggtt cgagtatggt tctgtaaccg
     2461 gcgccagaag ggcaaaagat caagtattga gtattcccaa cgagaagagt atgaggctac
     2521 agggacacct ttcccagggg gggctgtatc ctttcctctg cccccaggtc cccactttgg
     2581 caccccaggc tatggaagcc cccacttcac cacactctac tcagtccctt ttcctgaggg
     2641 cgaggccttt ccctctgttc ccgtcactgc tctgggctct cccatgcatt caaacgggga
     2701 tctcaaaatt gtcgctcctg tcaaacaaac tcttaacttt gatttactca aactggctgg
     2761 ggatgtagaa agcaatccag gtccactcat ggctgtcagc gacgctctgc tcccgtcctt
     2821 ctccacgttc gcgtccggcc cggcgggaag ggagaagaca ctgcgtccag caggtgcccc
     2881 gactaaccgt tggcgtgagg aactctctca catgaagcga cttcccccac ttcccggccg
     2941 cccctacgac ctggcggcga cggtggccac agacctggag agtggcggag ctggtgcagc
     3001 ttgcagcagt aacaacccgg ccctcctagc ccggagggag accgaggagt tcaacgacct
     3061 cctggaccta gactttatcc tttccaactc gctaacccac caggaatcgg tggccgccac
     3121 cgtgaccacc tcggcgtcag cttcatcctc gtcttcccca gcgagcagcg gccctgccag
     3181 cgcgccctcc acctgcagct tcagctatcc gatccgggcc gggggtgacc cgggcgtggc
     3241 tgccagcaac acaggtggag ggctcctcta cagccgagaa tctgcgccac ctcccacggc
     3301 ccccttcaac ctggcggaca tcaatgacgt gagcccctcg ggcggcttcg tggctgagct
     3361 cctgcggccg gagttggacc cagtatacat tccgccacag cagcctcagc cgccaggtgg
     3421 cgggctgatg ggcaagtttg tgctgaaggc gtctctgacc acccctggca gcgagtacag
     3481 cagcccttcg gtcatcagtg ttagcaaagg aagcccagac ggcagccacc ccgtggtagt
     3541 ggcgccctac agcggtggcc cgccgcgcat gtgccccaag attaagcaag aggcggtccc
     3601 gtcctgcacg gtcagccggt ccctagaggc ccatttgagc gctggacccc agctcagcaa
     3661 cggccaccgg cccaacacac acgacttccc cctggggcgg cagctcccca ccaggactac
     3721 ccctacactg agtcccgagg aactgctgaa cagcagggac tgtcaccctg gcctgcctct
     3781 tcccccagga ttccatcccc atccggggcc caactaccct cctttcctgc cagaccagat
     3841 gcagtcacaa gtcccctctc tccattatca agagctcatg ccaccgggtt cctgcctgcc
     3901 agaggagccc aagccaaaga ggggaagaag gtcgtggccc cggaaaagaa cagccaccca
     3961 cacttgtgac tatgcaggct gtggcaaaac ctataccaag agttctcatc tcaaggcaca
     4021 cctgcgaact cacacaggcg agaaacctta ccactgtgac tgggacggct gtgggtggaa
     4081 attcgcccgc tccgatgaac tgaccaggca ctaccgcaaa cacacagggc accggccctt
     4141 tcagtgccag aagtgcgaca gggccttttc caggtcggac caccttgcct tacacatgaa
     4201 gaggcacttt ctcgagggct cgggccagtg tactaattat gctctcttga aattggctgg
     4261 agatgttgag agcaacccag gtcccggcgc gccactggat ttcctttggg cgttggaaac
     4321 cccgcagaca gccacgacga tgcccctcaa cgtgaacttc accaacagga actatgacct
     4381 cgactacgac tccgtacagc cctatttcat ctgcgacgag gaagagaatt tctatcacca
     4441 gcaacagcag agcgagctgc agccgcccgc gcccagtgag gatatctgga agaaattcga
     4501 gctgcttccc accccgcccc tgtccccgag ccgccgctcc gggctctgct ctccatccta
     4561 tgttgcggtc gctacgtcct tctccccaag ggaagacgat gacggcggcg gtggcaactt
     4621 ctccaccgcc gatcagctgg agatgatgac cgagttactt ggaggagaca tggtgaacca
     4681 gagcttcatc tgcgatcctg acgacgagac cttcatcaag aacatcatca tccaggactg
     4741 tatgtggagc ggtttctcag ccgctgccaa gctggtctcg gagaagctgg cctcctacca
     4801 ggctgcgcgc aaagacagca ccagcctgag ccccgcccgc gggcacagcg tctgctccac
     4861 ctccagcctg tacctgcagg acctcaccgc cgccgcgtcc gagtgcattg acccctcagt
     4921 ggtctttccc tacccgctca acgacagcag ctcgcccaaa tcctgtacct cgtccgattc
     4981 cacggccttc tctccttcct cggactcgct gctgtcctcc gagtcctccc cacgggccag
     5041 ccctgagccc ctagtgctgc atgaggagac accgcccacc accagcagcg actctgaaga
     5101 agagcaagaa gatgaggaag aaattgatgt ggtgtctgtg gagaagaggc aaacccctgc
     5161 caagaggtcg gagtcgggct catctccatc ccgaggccac agcaaacctc cgcacagccc
     5221 actggtcctc aagaggtgcc acgtctccac tcaccagcac aactacgccg cacccccctc
     5281 cacaaggaag gactatccag ctgccaagag ggccaagttg gacagtggca gggtcctgaa
     5341 gcagatcagc aacaaccgca agtgctccag ccccaggtcc tcagacacgg aggaaaacga
     5401 caagaggcgg acacacaacg tcttggaacg tcagaggagg aacgagctga agcgcagctt
     5461 ttttgccctg cgtgaccaga tccctgaatt ggaaaacaac gaaaaggccc ccaaggtagt
     5521 gatcctcaaa aaagccaccg cctacatcct gtccattcaa gcagacgagc acaagctcac
     5581 ctctgaaaag gacttattga ggaaacgacg agaacagttg aaacacaaac tcgaacagct
     5641 tcgaaactct ggtgcagggg atctcaaaat tgtcgctcct gtcaaacaaa ctcttaactt
     5701 tgatttactc aaactggctg gggatgtaga aagcaatcca ggtccactca tgaacaattc
     5761 gcccttcacc atgtataaca tgatggagac ggagctgaag ccgccgggcc cgcagcaagc
     5821 ttcggggggc ggcggcggag gaggcaacgc cacggcggcg gcgaccggcg gcaaccagaa
     5881 gaacagcccg gaccgcgtca agaggcccat gaacgccttc atggtatggt cccgggggca
     5941 gcggcgtaag atggcccagg agaaccccaa gatgcacaac tcggagatca gcaagcgcct
     6001 gggcgcggag tggaaacttt tgtccgagac cgagaagcgg ccgttcatcg acgaggccaa
     6061 gcggctgcgc gctctgcaca tgaaggagca cccggattat aaataccggc cgcggcggaa
     6121 aaccaagacg ctcatgaaga aggataagta cacgcttccc ggaggcttgc tggcccccgg
     6181 cgggaacagc atggcgagcg gggttggggt gggcgccggc ctgggtgcgg gcgtgaacca
     6241 gcgcatggac agctacgcgc acatgaacgg ctggagcaac ggcagctaca gcatgatgca
     6301 ggagcagctg ggctacccgc agcacccggg cctcaacgct cacggcgcgg cacagatgca
     6361 accgatgcac cgctacgacg tcagcgccct gcagtacaac tccatgacca gctcgcagac
     6421 ctacatgaac ggctcgccca cctacagcat gtcctactcg cagcagggca cccccggtat
     6481 ggcgctgggc tccatgggct ctgtggtcaa gtccgaggcc agctccagcc cccccgtggt
     6541 tacctcttcc tcccactcca gggcgccctg ccaggccggg gacctccggg acatgatcag
     6601 catgtacctc cccggcgccg aggtgccgga gcccgctgcg cccagtagac tgcacatggc
     6661 ccagcactac cagagcggcc cggtgcccgg cacggccatt aacggcacac tgcccctgtc
     6721 gcacatgtga gggctggaag ggcgaattgc ctgcaggaat tcgcggccgc actcgagata
     6781 tctagaccca gctttcttgt acaaagtggt gatctagcta aaacgcggcc tcgaatcgaa
     6841 ttccgccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     6901 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     6961 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     7021 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     7081 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     7141 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     7201 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     7261 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     7321 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     7381 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat
     7441 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     7501 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     7561 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     7621 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     7681 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     7741 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     7801 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     7861 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     7921 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     7981 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     8041 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     8101 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa gcggccgcga
     8161 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     8221 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     8281 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     8341 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg
     8401 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac
     8461 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa
     8521 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat
     8581 agctgtccct cttctcttat ggagatccct cgacctgcag gaattcgatt acttaagcct
     8641 aggatccaga tctgtacatt cgaatcgatg aattcgttaa ccatggaagc ttctgcagcg
     8701 gccgcactag aggtacccgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     8761 aacgaccccc gcccattgac gtcaatagta acgccaatag ggactttcca ttgacgtcaa
     8821 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     8881 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcattg tgcccagtac
     8941 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     9001 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
     9061 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
     9121 ggggggcgcg cgccrggsgg ggsggggsgg ggsgrggggs ggggsggggs grggsggrga
     9181 ggkgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
     9241 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
     9301 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
     9361 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagctg
     9421 agcaagaggt aagggtttaa gggatggttg gttggtgggg tattaatgtt taattacctg
     9481 gagcacctgc ctgaaatcac tttttttcag gttggaccgg tgccaccatg gactataagg
     9541 accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac gataagatgg
     9601 ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgac aagaagtaca
     9661 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     9721 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     9781 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
     9841 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     9901 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     9961 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
    10021 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
    10081 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
    10141 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
    10201 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
    10261 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
    10321 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
    10381 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
    10441 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
    10501 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
    10561 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
    10621 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
    10681 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
    10741 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
    10801 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
    10861 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
    10921 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
    10981 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
    11041 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
    11101 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
    11161 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
    11221 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
    11281 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
    11341 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
    11401 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
    11461 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
    11521 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
    11581 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
    11641 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
    11701 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
    11761 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
    11821 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
    11881 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
    11941 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
    12001 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
    12061 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
    12121 aggaactgga catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct
    12181 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
    12241 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
    12301 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
    12361 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
    12421 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
    12481 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
    12541 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
    12601 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
    12661 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
    12721 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
    12781 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
    12841 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
    12901 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
    12961 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
    13021 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
    13081 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
    13141 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
    13201 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
    13261 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
    13321 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
    13381 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
    13441 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
    13501 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
    13561 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
    13621 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
    13681 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
    13741 gaggcgacaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagtaag
    13801 aattcctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
    13861 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
    13921 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
    13981 ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg ctggggagcg
    14041 gccgcactag tctagtctag agcgatcgcc cgggtttaaa cgctagccaa caagctcgtc
    14101 atcgctttgc agaagagcag agaggatatg ctcatcgtct aaagaactac ccattttatt
    14161 atatattagt cacgatatct ataacaagaa aatatatata taataagtta tcacgtaagt
    14221 agaacatgaa ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga
    14281 taatcatgcg tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg
    14341 cattgacaag cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag
    14401 cgacggattc gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt
    14461 tacgcagact atctttctag ggttaaaaaa gatttgcgct ttactcgacc taaactttaa
    14521 acacgtcata gaatcttcgt ttgacaaaaa ccacattgtg gccaagctgt gtgacgcgac
    14581 gcgcgctaaa gaatggcaaa ccaagtcgcg cgaggtacca gcttttgttc cctttagtga
    14641 gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    14701 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    14761 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    14821 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    14881 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    14941 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    15001 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    15061 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    15121 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    15181 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    15241 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    15301 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    15361 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    15421 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    15481 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    15541 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    15601 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    15661 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    15721 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    15781 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    15841 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    15901 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    15961 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    16021 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    16081 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    16141 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    16201 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    16261 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    16321 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    16381 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    16441 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    16501 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    16561 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    16621 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    16681 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    16741 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    16801 tttccccgaa aagtgccacc t
//
