LOCUS       sequence-236056-        2969 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin
            resistance protein). Please see Supplemental Documents for annotated
            Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2969)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C77m
FEATURES             Location/Qualifiers
     source          1..2969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    601..621
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(627..644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(798..1386)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(878..897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1557..2348,2349..2417))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2418..2522)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2590..2608
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2612..2655)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2658..2679
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             join(2698..2766,2767..2969,1..589)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
       61 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      121 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      181 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      241 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
      301 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      361 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      421 taaatctgga gccggtgagc gtgggtcgcg cggtatcatt gcagcactgg ggccagatgg
      481 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
      541 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaaa ggtagagacc
      601 tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      661 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      721 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      781 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
      841 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      901 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      961 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1021 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1081 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1141 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1201 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1261 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1321 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1381 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1441 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1501 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1561 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1621 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1681 gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc aataaaccag
     1741 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1801 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1861 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1921 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1981 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2041 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2101 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2161 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2221 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2281 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2341 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2401 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     2461 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2521 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2581 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
     2641 gctaaggatg atttctggaa ttcgcggccg cttctagaga ctagtgggtc tcaaatgatg
     2701 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2761 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2821 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2881 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2941 attgacgccg ggcaagagca actcggtcg
//
