LOCUS       sequence-236069-        5155 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CAG:mcherry:P2A:HA_NLGN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5155)
  TITLE     SYNPLA plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: jk96
FEATURES             Location/Qualifiers
     source          1..5155
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        313..590
                     /label=chicken beta-actin promoter
     primer_bind     869..891
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     953..972
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1005..1014
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1011..1718
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1156..1174)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1424..1443
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1731..1787
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1929..1955
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      2417..2426
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4365..4953
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4418..4438)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4836..4847)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4996..5117
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5007..5026)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5061..5080
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct aggaagatcg
       61 taccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      121 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      181 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      241 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      301 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
      361 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
      421 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
      481 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
      541 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg
      601 cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
      661 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta
      721 attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg
      781 ggctccggga gggccctttg tgcgggggga gcggctcggg gctgtccgcg gggggacggc
      841 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct
      901 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
      961 ctggttattg tgctgtctca tcattttggc aaagaattgg atctgccacc atggtgagca
     1021 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     1081 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     1141 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     1201 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     1261 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     1321 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     1381 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     1441 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     1501 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     1561 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     1621 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     1681 gccgccactc caccggcggc atggacgagc tgtacaagtc tggaagcgga gctactaact
     1741 tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctatg gcacttccca
     1801 gatgcatgtg gccaaattat gtttggagag ctatgatggc atgtgtggtc cacaggggat
     1861 ccggtgcccc attgactctc tgcttgttgg gatgtttgct acagactttt cacgtactct
     1921 ctcaaaagta tccatacgac gttccggact acgcagtcga cttggatgat gtagacccat
     1981 tggttactac taactttggc aagattaggg gaattaagaa agaactcaat aatgaaattt
     2041 tgggtcctgt cattcagttt cttggggttc catatgccgc tccaccaaca ggagaacatc
     2101 gtttccagcc tccagaacca ccatctccct ggtctgacat ccggaacgcc actcagtttg
     2161 ctcctgtatg tccccagaat atcattgatg gcagattgcc tgaagttatg cttcctgtgt
     2221 ggttcactaa taacttggat gtggtttcat catacgtcca agaccagagt gaagactgtc
     2281 tatacttaaa catctatgtc ccaactgaag atggtcccct tacaaagaaa cacacagatg
     2341 atttaggtga taatgacggt gctgaagatg aagatattcg ggacagtggg ggtcccaaac
     2401 cagtgatggt gtacatccat ggcggctctt acatggaagg tactggaaat ctgtatgatg
     2461 ggagtgtctt ggcaagctat ggcaatgtga tcgtcatcac agtcaactat cggcttgggg
     2521 tacttggctt cttgagcaca ggggatcagg ctgccaaagg aaactacggg ctccttgacc
     2581 tcatccaggc cctaagatgg accagcgaga acattgggtt ctttggtggt gaccccttgc
     2641 gaatcactgt gtttggatca ggcgctgggg gttcatgtgt caacctgctg actttatccc
     2701 attattctga aggtaaccgt tggagcaatt caaccaaagg actttttcaa cgagcaatag
     2761 ctcagagtgg aacagccctt tccagctggg ctgttagttt ccagcctgca aaatacgcta
     2821 gaattctggc cacaaaagtt ggctgcaatg tttcagatac agtagagtta gtagaatgcc
     2881 tgcagaagaa gccttacaaa gaacttgttg atcaagatgt tcaaccagcc cgataccaca
     2941 tagcctttgg acctgtgatc gatggtgatg taataccaga tgaccctcag atactgatgg
     3001 aacaaggaga gttcctcaac tatgatataa tgttgggagt taaccaaggg gaagggttga
     3061 agtttgtcga aaatatagta gatagtgatg atggtgtatc agccagtgat ttcgactttg
     3121 ctgtttctaa ttttgttgat aatttatatg gatatcctga aggcaaagat gttttgagag
     3181 aaaccattaa attcatgtat actgactggg ctgatcgcca taatcctgaa actagaagga
     3241 agacattgtt ggctttgttt acggaccatc aatgggtagc acctgctgtg gccacagcag
     3301 accttcactc gaactttggc tcacctacat acttctatgc cttttatcat cattgccaaa
     3361 cagatcaagt tccagcttgg gctgatgcag ctcatgggga tgaggttccc tatgtgttgg
     3421 gaatccccat gattggccct acagagttat ttccttgcaa tttctccaag aatgatgtga
     3481 tgttgagtgc agtagtaatg acatactgga cgaattttgc taaaactggt gacccaaatc
     3541 aaccagttcc tcaagacaca aaattcatcc ataccaaacc caaccgcttt gaagaagtag
     3601 catggaccag atattcccag aaagaccagc tttatctcca tattggatta aaaccgagag
     3661 ttaaagagca ttacagagcc aataaggtaa atctctggct ggagctggta cctcatctgc
     3721 ataatctcaa tgacatttct cagtatacct cgacaacaac taaagtgcca tccacggaca
     3781 tcactctcag acctacaagg aagaattcca caccagtcac atcagccttt cccactgcca
     3841 aacaggatga tcccaagcaa caaccaagcc ccttctcggt ggatcagagg gactactcca
     3901 cagagctaag tgtcactatc gcagtggggg cctctctgct gtttctcaac atcttggctt
     3961 ttgcagccct gtactacaag aaggataaga ggagacatga tgtccaccgg aggtgcagcc
     4021 ctcagcgcac gaccaccaac gacctaaccc atgctccaga agaggaaatt atgtctctcc
     4081 aaatgaagca cactgacttg gatcacgagt gtgagtccat ccatccacat gaggtggttc
     4141 ttcggaccgc ctgtccccca gattatactc tagctatgag gaggtcacct gatgatattc
     4201 cactaatgac acctaacacc atcacaatga ttcccaacac tataccaggg attcagccct
     4261 tacatacatt caacacattt actggaggac agaataatac actgccccat ccccacccac
     4321 acccccattc acattcaaca accagggtat agaagcttat cgataatcaa cctctggatt
     4381 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4441 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4501 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4561 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4621 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4681 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4741 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     4801 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4861 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4921 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgaccc gggcggccgc
     4981 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     5041 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     5101 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttca
//
