LOCUS       sequence-236071-        4743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of mCherry on pAL5000/pMB1 backbone with 
            kanamycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   DeLorenzo DM, Henson WR, Moon TS
  TITLE     Development of Chemical and Metabolite Sensors for Rhodococcus 
            opacus PD630.
  JOURNAL   ACS Synth Biol. 2017 Oct 20;6(10):1973-1978. doi: 
            10.1021/acssynbio.7b00192. Epub 2017 Aug 10.
  PUBMED    28745867
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00192"; journalName: "ACS Synth Biol"; date: 
            "2017-10-20- 20"; volume: "6"; issue: "10"; pages: "1973-1978"
COMMENT     Sequence Label: pDD103
FEATURES             Location/Qualifiers
     source          1..4743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..711
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(146..164)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     414..433
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(738..755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(909..1497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(989..1008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1781..2575)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1892..1911)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2502..2521
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     terminator      4448..4475
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      301 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      421 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
      481 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      601 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta acctgaattc
      721 gagctggatc cagcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      781 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      841 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1021 ctcgtgcgct ctcctgtttc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1321 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttggg gtgggcgaag
     1621 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
     1681 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
     1741 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     1801 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     1861 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     1921 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     1981 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     2041 gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     2101 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     2161 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     2221 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     2281 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     2341 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttggagtt
     2401 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     2461 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     2521 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     2581 acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg
     2641 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag
     2701 ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa
     2761 gcccactgca agctacctgc tttctctttg cgcttgcgtc tgacattcat ccggggtcag
     2821 caccgtttct gcggactggc tttctacgtg ttccgcttcc tttagcagcc cttgcgccct
     2881 gagtgcttgc ggcagcgtga agctagcgtc taccaggact tttacctgtc cgacccgttg
     2941 caacggaacc ccccacggaa cccccgcgac acccgctccc caattgcgtt agaacagcgg
     3001 tggattgtcg gcttcgttgt gggccttttg agccgcttcc tgttctgccg cacgctcttt
     3061 cctcgcccga tagccgagtc gcttaacggt gtccagatgc agcccgaaat gtttggccgt
     3121 ttgcggccaa gagtggccct cgtcgtcgtg ataggcgcgg atgcgttcgc ggcgtgcagc
     3181 ctgctcggcg agccactcgc tgcgttcctg cgccacgagc cggacgacgt ggcgttcgga
     3241 tagtccggtg attcgagcgc cttcggcggc ggtcacgcgc cgctttttgc ggacagtcgg
     3301 ctgccggttg tagccgtcgc tgtagccgtc gctcatagca atgcctccat ggctgacgcg
     3361 gactttgcgc gccgcgcaac tgtgctcgcc gccgtgcgcg ctgctgcgcc cttccgcgag
     3421 atggccgact ggcgcgcact gagtgtggcc tcgtagacca cgatcccgtc cgcccaaatg
     3481 cgcgacttgg ttgtgatcca acgccaaatg ctgttggcga tggcgcggac ctcgctgtcc
     3541 ggtagcggtc cgggacacac gtcgttgcac gggaactcgg cgtttcgcgc gtggcactcg
     3601 gcatagatcg cgcggccgag tccgtccacg ttccgggtcg gcaggtagat ccgcatgagg
     3661 gcgggacgat aggcccacaa cctgacggaa tcgaacagtg cgcaattccg ccctagcggc
     3721 gtcggagccg ctttgtacgt ggtctgctga cgccagcgcg gcggtggcat gttcgcgccg
     3781 agctcggcct cgatgtggct gagtgtgtag agatctgagt ggagccattc cgtttcccag
     3841 gcgatgtggc cggggttttt ggtcatgagg cctgagtaac tgcggtcgcc gtccacggcg
     3901 cgccgaaggc cttcggcgca cgccgccatg tatgcgagcg gcttacgccg cgcgtattcg
     3961 gtgcgtggaa caggggcgtt gagtgcccac actgcgtgtg cgtggccgtt ggcgcgattg
     4021 cccacgatcg cgttgggcag cggatgggac ccccgggcgc tgagcgctcg gagcgctgcg
     4081 tctggatggt ctacgtccac gaccagcagg tttgccagcg ctgttgggtt cgcctcgatg
     4141 taccggcggc ctagggccga cgcgcggctt tggcggtaga tcccctcgag cagatcgtcg
     4201 cttgccagcg gccagtacgg cagccagagc tgctcaaatt cgtcggcgac gtggctcacg
     4261 cttggtagta gaccacgatt aatcaccggt gtatggtccg acacgagctc caagtcagat
     4321 atttcgctga ggggccaccc cacaactgca cactcccccg ctctcccgtc gagccctggt
     4381 ggtggaacac cagcgacagc cgagcacccc caaccacctg taccaaccac tagttgatta
     4441 gctaagcaga aggccatcct gacggatggc ctttttgcgt ttaatactgt ttaaactcta
     4501 gaaatattgg atcgtcggca ccgtcacggc cgtgggaggc ggcacgatcc gcgacgtgat
     4561 gatcggccgc atccccacgg tgctgcgcag tgagctctac gccatcccgg cgttgatctg
     4621 tgcgttcgct gtgcgggctc taacacgtcc tagtatggta ggatgagcaa catttcgacg
     4681 ccgagagatt cgccgcccga aatgggcacg atccgcatgc ttaattaaga aggagatata
     4741 cat
//
