LOCUS       sequence-236073-       14843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ptet-on::cexA::ttrpC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14843)
  AUTHORS   Steiger MG, Rassinger A, Mattanovich D, Sauer M
  TITLE     Engineering of the citrate exporter protein enables high citric acid
            production in Aspergillus niger.
  JOURNAL   Metab Eng. 2019 Mar;52:224-231. doi: 10.1016/j.ymben.2018.12.004. 
            Epub 2018 Dec 13.
  PUBMED    30553933
REFERENCE   2  (bases 1 to 14843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-03"; volume: "52:224-231. doi"; pages: " 
            10.1016/j.ymben.2018.12.004. Epub 2018 Dec 13"
COMMENT     Sequence Label: pMST-713
FEATURES             Location/Qualifiers
     source          1..14843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          639..754
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             join(772..1389,1390..1395,1396..1431,1432..1434,1435..1470,
                     1471..1473,1474..1509,1510..1518)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    join(1866..1884,1885..1907,1908..1926,1927..1949,1950..1968,
                     1969..1991,1992..2010,2011..2033,2034..2052,2053..2075,
                     2076..2094,2095..2117,2118..2136)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     intron          2206..2321
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     terminator      3920..4693
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene (Punt et al., 1987)"
     regulatory      9740..9749
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10333..11358
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             10333..11358
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(12021..12037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12021..12037)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      12085..12116
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        12117..12219
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             12220..13035
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(12293..12312)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      13059..13086
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(13098..13685)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13178..13197)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13725..13742
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13726..13742
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cagtcaatgg caaggaaact ggagcgcaaa aaaccccgcc cctgacaggg cggggttttt
       61 tcgcgatcgg agtcgataag cttcggagaa tatggagctt catcgaatca ccggcagtaa
      121 gcgaaggaga atgtgaagcc aggggtgtat agccgtcggc gaaatagcat gccattaacc
      181 taggtacaga agtccaattg cttccgatct ggtaaaagat tcacgagata gtaccttctc
      241 cgaagtaggt agagcgagta cccggcgcgt aagctcccta attggcccat ccggcatctg
      301 tagggcgtcc aaatatcgtg cctctcctgc tttgcccggt gtatgaaacc ggaaaggccg
      361 ctcaggagct ggccagcggc gcagaccggg aacacaagct ggcagtcgac ccatccggtg
      421 ctctgcactc gacctgctga ggtccctcag tccctggtag gcagctttgc cccgtctgtc
      481 cgcccggtgt gtcggcgggg ttgacaaggt cgttgcgtca gtccaacatt tgttgccata
      541 ttttcctgct ctccccacca gctgctcttt tcttttctct ttcttttccc atcttcagta
      601 tattcatctt cccatccaag aacctttatt tcccctaagt aagtactttg ctacatccat
      661 actccatcct tcccatccct tattcctttg aacctttcag ttcgagcttt cccacttcat
      721 cgcagcttga ctaacagcta ccccgcttga gcagacatca ccgaattcac catgtctaga
      781 ctggacaaga gcaaagtcat aaacggcgct ctggaattac tcaatggagt cggtatcgaa
      841 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
      901 cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat
      961 catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc
     1021 aagtcattcc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
     1081 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa
     1141 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc
     1201 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc
     1261 gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa
     1321 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
     1381 gaaagcggcg ggccggccga cgcccttgac gattttgact tagacatgct cccagccgat
     1441 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
     1501 gacatgctcc ccgggtaact aagtaaggat ccactagtac agcagaagaa tctctctccg
     1561 ctgttgcttc agtgtctgcc atgcattaac ttcatcctac tgtcctaccc gcagtaccca
     1621 ttcacatttg cggcagatac caggttgttt tctattccct cggttcttca gttcttcaga
     1681 tattacatta ctcgagaaat tggcggcgat gcaggagttt ggttggtttt aattgtttca
     1741 gtctccttga cctgatttgt atgacatgca atgtttcgac ggatgactat ctcggtgaat
     1801 atacgtcatg aatcatgcgg ccgcgcgtat cacgaggccc tttcgacttc actcgagttt
     1861 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     1921 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     1981 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2041 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2101 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctccccatc
     2161 ttcagtatat tcatcttccc atccaagaac ctttatttcc cctaagtaag tactttgcta
     2221 catccatact ccatccttcc catcccttat tcctttgaac ctttcagttc gagctttccc
     2281 acttcatcgc agcttgacta acagctaccc cgcttgagca gacatcaccg tttaaacacc
     2341 atgtcctcaa ccacgtcctc atcaagatca gaccttgaaa aggtccccgt accacaggtc
     2401 acccctagag acagtgactc cgataaggga tccctctctc cggagccttc gaccctcgag
     2461 gctcagtcat ccgagaagcc accgcatcat atcttcacac gatctcgcaa gctgcaaatg
     2521 gtttgcatcg tctccctcgc tgccatattt tctccgcttt cgtcgaacat ttacttccct
     2581 gccctggatg atgtctcgaa atccctcaac atcagcatgt cgctcgcaac actcaccatc
     2641 acggtgtaca tgatcgtcca aggcctcgct cccagcttct ggggttccat gtcagacgcc
     2701 acaggtagac ggcctgtctt tattggaaca ttcattgttt acctcgtagc caatattgct
     2761 ctggccgaat ccaagaacta tggtgagctc atggccttcc gagccttgca ggctgctggt
     2821 agcgcagcca ccatctcaat cggagctgga gtgattggtg atatcacaaa ctcggaagaa
     2881 agaggtagct tggtgggtat cttcggtgga gttcgcatgc ttggacaggg aatcgggccg
     2941 gttttcggcg gcattttcac ccagtatctc ggatatcgat ctatcttttg gttcctcacg
     3001 attgctggag gcgtgagtct cctgtccatt ctggtgcttc ttccggagac attgaggcca
     3061 attgctggaa atggaactgt gaagctcaat ggcattcata agcccttcat ctacacgatc
     3121 accggccaga cgggggttgt cgagggagcg caaccggaag cgaaaaagac caaaaccagc
     3181 tggaagtctg tttttgctcc tttgacattc ctcgtcgaaa aggacgtttt catcaccctg
     3241 ttctttggaa gtatcgtgta cacagtgtgg agcatggtga catccagtac caccgacctc
     3301 ttcagcgaag tgtacggcct gtcatccctg gacattggac tcactttcct aggcaatggc
     3361 tttggatgta tgtctggctc ttatctggtc ggctacctta tggattacaa ccaccgtctt
     3421 accgaacgcg aatattgcga gaaacacggt tatccggcag gcacacgtgt caatctgaaa
     3481 tcacaccccg acttccccat tgaggtcgcc cggatgcgca atacctggtg ggtgattgcg
     3541 atcttcatcg tgacagttgc tttgtacggc gtgtctttgc ggacacatct ggcggtgcct
     3601 atcattctgc agtacttcat tgcgttctgc tcaacaggac tcttcaccat caacagcgcc
     3661 ctggtcatcg atctttaccc aggtgctagc gccagtgcga cagcagtgaa caatctgatg
     3721 cggtgcctgc ttggagctgg cggtgtggct atcgtgcaac ctatcctgga cgccttgaag
     3781 ccggattata ctttcctctt gcttgccggc atcacccttg tgatgactcc gttgctgtac
     3841 gtcgaagatc gatggggtcc tggctggcga catgcccgcg aaaggagact caaggccaaa
     3901 gccaacggca actaggcttg atccacttaa cgttactgaa atcatcaaac agcttgacga
     3961 atctggatat aagatcgttg gtgtcgatgt cagctccgga gttgagacaa atggtgttca
     4021 ggatctcgat aagatacgtt catttgtcca agcagcaaag agtgccttct agtgatttaa
     4081 tagctccatg tcaacaagaa taaaacgcgt ttcgggttta cctcttccag atacagctca
     4141 tctgcaatgc attaatgcat tggacctcgc aaccctagta cgcccttcag gctccggcga
     4201 agcagaagaa tagcttagca gagtctattt tcattttcgg gagacgagat caagcagatc
     4261 aacggtcgtc aagagtccta cgagactgag gaatccgctc ttggctccac gcgactatat
     4321 atttgtctct aattgtactt tgacatgctc ctcttcttta ctctgatagc ttgactatga
     4381 aaattccgtc accagcccct gggttcgcaa agataattgc actgtttctt ccttgaactc
     4441 tcaagcctac aggacacaca ttcatcgtag gtataaacct cgaaaatcat tcctactaag
     4501 atgggtatac aatagtaacc atgcatggtt gcctagtgaa tgctccgtaa cacccaatac
     4561 gccggccgaa acttttttac aactctccta tgagtcgttt acccagaatg cacaggtaca
     4621 cttgtttaga ggtaatcctt ctttctagaa gtcctcgtgt actgtgtaag cgcccactcc
     4681 acatctccac tcgctccggg gagacgggca caaggttcat gaaccgaaga ggatgggaac
     4741 cccgaccatg gcctgcggcc gcagctatca gtcctctcag ctgacgcccc tcacgaaccc
     4801 tcgcactctc ccgaaatgct cttcggtgtc catggcgcat catctcagca gcatcccatg
     4861 tcaaaccatg gctttgggcc cacggactct gtggccctgc cgcaacatca ccatcaccat
     4921 cgactcccgc cccatgcagc gctgcgcgcc ccagggcgtc atggagtgaa tgtggaatct
     4981 cctcctttgc cctcgggccc gccgagtgtg gtgggccagc ctggaatgcc tgatccagcc
     5041 cccaggcctc gaggaccaaa actgaagttt actcccgaag aggacgctct actggtggag
     5101 ttgaaggaaa acaagaactt gacgtggaag caaattgcag acttcttccc gggccgaacg
     5161 agcggtacct tgcaagtccg atactgcacc aagctgaagg ctaaggatgt agcttggagt
     5221 gacgaaatgg ttcgatttgc tcctgatgta tttctacgcc tgtctcacac atgctaatga
     5281 agggaatagg tacaaaggct gcagcgggca atgcacgagt acgagaacga tcggtggcgc
     5341 atcattgcag ggaaggttgg aaatggcttc accccagctg cttgccgcga gaaagccatg
     5401 cagctccatg agtaaaagcg ttggggaatt ttcatattta tatctactgt cgccagattc
     5461 ggccctgctt ggaccctctg atctccttac tctccatatt ggttcaaatg tcgggtcacc
     5521 gatagggctg gtggtgcagg cttgttgtag gcacgggagg atgatcagca taactctgag
     5581 tcactatagg gacgggttga tgtaaggtat taagtgatgt atgataattc attttagccc
     5641 gggggaacat atggcgccgg catttgttcg ttcgcaatga accgacacta gcgtccgctc
     5701 tcgcagttta gcaccggctg atcccgggct gaacgcggcc attgctcggc cggggcatgt
     5761 gttccttatc tacggcagac cgcagatgac cactggagca gattatagac cctaagccct
     5821 aagccggaca cccaatcgag taggtctgcg gaccaggtca ctgcgggcag ccggagaagc
     5881 tccgcaacca atcaatcccc ggcgctgact aagggcaggc gaccacgggc cgaagcggct
     5941 tcaaactcac ctcaacctcc aaactccctc atctccaaac gtccttgcct tgtctgccgt
     6001 cattgcaacc cacccaccag gacacatgag cagcaagtcc cagctcacct acactgcccg
     6061 tgccagcaag catcccaatg ctctggcgaa gaggctgttc gagattgccg aggccaagaa
     6121 aaccaatgtg actgtctcgg ctgacgttac caccactaag gagctactag atcttgctga
     6181 ccgtctcggt ccctacattg ccgtgatcaa aacccacatc gatatcctct ctgatttcag
     6241 caacgagact attgagggac ttaaggctct cgcgcagaag cacaactttc tcatcttcga
     6301 ggaccgcaag ttcattgaca tcggcaacac ggtccagaag caataccacg gcggtaccct
     6361 ccgtatctcg gaatgggccc acatcatcaa ctgcagcatt ctccctggtg agggtatcgt
     6421 cgaggctctc gctcagacgg cgtctgcacc ggacttcgcc tacggccccg aacgcggtct
     6481 gttgatcttg gcagagatga cctctaaggg ctccttggct accggccagt acactacttc
     6541 ctcggtcgat tatgcccgga aatacaagaa cttcgttatg ggattcgtgt cgacgcgcgc
     6601 gttgggtgag gtgcagtcgg aagtcagctc tccttcggat gaggaggact ttgtggtttt
     6661 cacgactggt gtgaacatta acctccaagt cgcaattgac ctacgcttat gctgcagacg
     6721 cgttacgtat cggatccaga attcgtgata tctgaattcg tcgacaagct tattttttgt
     6781 atactgtttt gtgatagcac gaagtttttc cacggtatct tgttaaaaat atatatttgt
     6841 ggcgggctta cctacatcaa attaataaga gactaattat aaactaaaca cacaagcaag
     6901 ctactttagg gtaaaagttt ataaatgctt ttgacgtata aacgttgctt gtatttatta
     6961 ttacaattaa aggtggatag aaaacctaga gactagttag aaactaatct caggtttgcg
     7021 ttaaactaaa tcagagcccg agaggttaac agaacctaga aggggactag atatccgggt
     7081 agggaaacaa aaaaaaaaaa caagacagcc acatattagg gagactagtt agaagctagt
     7141 tccaggacta ggaaaataaa agacaatgat accacagtct agttgacaac tagatagatt
     7201 ctagattgag gccaaagtct ctgagatcca ggttagttgc aactaatact agttagtatc
     7261 tagtctccta taactctgaa gctagaataa cttactacta ttatcctcac cactgttcag
     7321 ctgcgcaaac ggagtgattg caaggtgttc agagactagt tattgactag tcagtgacta
     7381 gcaataacta acaaggtatt aacctaccat gtctgccatc accctgcact tcctcgggct
     7441 cagcagcctt ttcctcctca ttttcatgct cattttcctt gtttaagact gtgactagtc
     7501 aaagactagt ccagaaccac aaaggagaaa tgtcttacca ctttcttcat tgcttgtctc
     7561 ttttgcatta tccatgtctg caactagtta gagtctagtt agtgactagt ccgacgagga
     7621 cttgcttgtc tccggattgt tggaggaact ctccagggcc tcaagatcca caacagagcc
     7681 ttctagatga ctggtcaata actagttggt ctttgtctga gtctgactta cgaggttgca
     7741 tactcgctcc ctttgcctcg tcaatcgatg agaaaaagcg ccaaaactcg caatatggct
     7801 ttgaaccaca cggtgctgag actagttaga atctagtccc aaactagctt ggatagctta
     7861 cctttgccct ttgcgttgcg acaggtcttg cagggtatgg ttcctttctc accagctgat
     7921 ttagctgcct tgctaccctc acggcggatc tgcataaaga gtggctagag gttataaatt
     7981 agcactgatc ctaggtacgg ggctgaatgt aacttgcctt tcctttctca tcgcgcggca
     8041 agacaggctt gctcaaattc ctaccagtca caggggtatg cacggcgtac ggaccacttg
     8101 aactagtcac agattagtta gcaactagtc tgcattgaat ggctgtactt acgggccctc
     8161 gccattgtcc tgatcatttc cagcttcacc ctcgttgctg caaagtagtt agtgactagt
     8221 caaggactag ttgaaatggg agaagaaact cacgaattct cgacaccctt agtattgtgg
     8281 tccttggact tggtgctgct atatattagc taatacacta gttagactca cagaaactta
     8341 cgcagctcgc ttgcgcttct tggtaggagt cggggttggg agaacagtgc cttcaaacaa
     8401 gccttcatac catgctactt gactagtcag ggactagtca ccaagtaatc tagataggac
     8461 ttgcctttgg cctccatcag ttccttcata gtgggaggtc cattgtgcaa tgtaaactcc
     8521 atgccgtggg agttcttgtc cttcaagtgc ttgaccaata tgtttctgtt ggcagaggga
     8581 acctgtcaac tagttaataa ctagtcagaa actagtatag cagtagactc actgtacgct
     8641 tgaggcatcc cttcactcgg cagtagactt catatggatg gatatcaggc acgccattgt
     8701 cgtcctgtgg actagtcagt aactaggctt aaagctagtc gggtcggctt actatcttga
     8761 aatccggcag cgtaagctcc ccgtccttaa ctgcctcgag atagtgacag tactctgggg
     8821 actttcggag atcgttatcg cgaatgctcg gcatactaat cgttgactag tcttggacta
     8881 gtcccgagca aaaaggattg gaggaggagg aggaaggtga gagtgagaca aagagcgaaa
     8941 taagagcttc aaaggctatc tctaagcagt atgaaggtta agtatctagt tcttgactag
     9001 atttaaaaga gatttcgact agttatgtac ctggagtttg gatataggaa tgtgttgtgg
     9061 taacgaaatg taagggggag gaaagaaaaa gtcggtcaag aggtaactct aagtcggcca
     9121 ttcctttttg ggaggcgcta accataaacg gcatggtcga cttagagtta gctcagggaa
     9181 tttagggagt tatctgcgac caccgaggaa cggcggaatg ccaaagaatc ccgatggagc
     9241 tctagctggc ggttgacaac cccacctttt ggcgtttctg cggcgttgca ggcgggactg
     9301 gatacttcgt agaaccagaa aggcaaggca gaacgcgctc agcaagagtg ttggaagtga
     9361 tagcatgatg tgccttgtta actaggtcaa aatctgcagt atgcttgatg ttatccaaag
     9421 tgtgagagag gaaggtccaa acatacacga ttgggagagg gcctaggtat aagagttttt
     9481 gagtagaacg catgtgagcc cagccatctc gaggagatta aacacgggcc ggcatttgat
     9541 ggctatgtta gtaccccaat ggaaagcctg agagtccagt ggcgctaatt catgaataac
     9601 ggtgagacta gcggccggtc cccttatccc agctgttcca cgttggcctg cccctcagtt
     9661 agcgctcaac tcaatgcccc tcactggcga ggcgagggca aggatggagg ggcagcatcg
     9721 cctgagttgg agcaaagcgg ccgccatggg agcagcgaac caacggaggg atgccgtgct
     9781 ttgtcgtggc tgctgtggcc aatccgggcc cttggttggc tcacagagcg ttgctgtgag
     9841 tccatgagct attattgcta ggtacagtat agagagagga gagagagaga gagagagaga
     9901 ggggaaaaaa ggtgaggttg aagtgagaaa aaaaaaaaaa aaaatccaac cactgacggc
     9961 tgccggctct gccacccccc tccctccacc ccagaccacc tgcacactca gcgcgcagca
    10021 tcacctaatc ttggctcgcc ttcccgcagc tcaggttgtt ttttttttct ctctccctcg
    10081 tcgaagccgc ccttgttccc ttatttattt ccctctccat ccttgtctgc ctttggtcca
    10141 tctgcccctt tgtctgcatc tcttttgcac gcatcgcctt atcgtcgtct cttttttcac
    10201 tcacgggagc ttgacgatga cctgactcgt gagcctcacc tgctgatttc tctccccccc
    10261 tcccgaccgg cttgactttt gtttctcctc cagtacctta tcgcgaagcc ggaagaacct
    10321 cttaacctct agatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gttcctgatc
    10381 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
    10441 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
    10501 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
    10561 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
    10621 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
    10681 gaggccatgg atgcgatcgc tgcggccgat ctcagccaga cgagcgggtt cggcccattc
    10741 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
    10801 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
    10861 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
    10921 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
    10981 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
    11041 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca cccggagctt
    11101 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
    11161 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
    11221 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
    11281 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
    11341 ccgagggcaa aggaatagat gcatggcttt cgtgaccggg cttcaaacaa tgatgtgcga
    11401 tggtgtggtt cccggttggc ggagtctttg tctactttgg ttgtctgtcg caggtcggta
    11461 gaccgcaaat gagcaactga tggattgttg ccagcgatac tataattcac atggatggtc
    11521 tttgtcgatc agtagctagt gagagagaga gaacatctat ccacaatgtc gagtgtctat
    11581 tagacatact ccgagaataa agtcaactgt gtctgtgatc taaagatcga ttcggcagtc
    11641 gagtagcgta taacaactcc gagtaccagc gaaagcacgt cgtgacagga gcagggcttt
    11701 gccaactgcg caaccttgct tgaatgagga tacacggggt gcaacatggc tgtactgatc
    11761 catcgcaacc aaaatttctg tttatagatc aagctggtag attccaatta ctccacctct
    11821 tgcgcttctc catgacatgt aagtgcacgt ggaaaccata cccaaattgc ctacagctgc
    11881 ggagcatgag cctatggcga tcagtctggt catgttaacc agcctgtgct ctgacgttaa
    11941 tgcagaatag cttcgcgtga tcttacggca ttatacgtat gatcggtcca cgatcagcta
    12001 gattatctag tcagcttgat gtcatagctg tttcctgagg ctcaatactg accatttaaa
    12061 tcatacctga cctccatagc agaaagtcaa aagcctccga ccggaggctt ttgacttgat
    12121 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
    12181 tttgagttat cgagattttc aggagctaag gaagctaaaa tgagccatat tcaacgggaa
    12241 acgtcttgct tgaagccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
    12301 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc
    12361 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
    12421 gagatggtca ggctaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
    12481 atccgtactc ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc
    12541 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
    12601 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacggcga tcgcgtattt
    12661 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttggtgcgag tgattttgat
    12721 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa actcttgcca
    12781 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
    12841 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
    12901 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
    12961 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca cttgatgctc
    13021 gatgagtttt tctaatgagg acctaaatgt aatcacctgg ctcaccttcg ggtgggcctt
    13081 tctgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    13141 tgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    13201 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    13261 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    13321 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    13381 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    13441 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    13501 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    13561 ctgctgaagc cagttacctc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    13621 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    13681 ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca cccgtgtaaa acgacggcca
    13741 gtttatctag tcagcttgat tctagctgat cgtggaccgg aaggtgagcc agtgagttga
    13801 ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa tgatatgcgg cccgctgtag
    13861 gtcaattgcg acttggagga agggatccgc caaggatgct ctggatccgc atccggccgc
    13921 tcttgcgccc catcaatcgc ccgactataa atcgaactac tttcggcatc ttctagactt
    13981 cctaataccg cctagtcata gcagattcaa gctgagaaca ccacaagtaa atatcaccca
    14041 tcatgcttac cctgaccgtc cctgaaaact acgggtatgt gccaattcta caattccttg
    14101 cagacaatgc cattctcccc atgaagtctg atgctaacta tcctgcagct ctgtcattgc
    14161 cgtcgctctg ggtgccatcc ccgtcctgag cttcgtccat ggcgccgtcg tgtctcgtct
    14221 ccgcaaggaa gctgattgcc cctaccctca ctgctatgcg accgtagagc agtgcaagac
    14281 caacgtaagc caacctcaca caaacaggat tcctcgagct aacatacatt ccgaaccgtg
    14341 cagcccaagg ccgagcagtt caactgcgct cagcgcgctc atgccaactt ccttgagaac
    14401 tccagccaaa ctatgctctt cctcctggta gctggactga agtaccccca gttggcgact
    14461 ggcctcggaa gcatctgggt cctcggtcgc tcactgttcc tttacggata tgtgtactcc
    14521 ggcaagccgc ggggtcgcgg tcgtttgtac ggcagcttct acttgcttgc acagggagct
    14581 ctctggggct tgacgtcttt tggagttgcg agggagttga tttcctactt ctaagtttgg
    14641 actgaatccg tggtgtgatt gaggtgattg gcgatgtttg gctataccag ctatatgtaa
    14701 taatctctac tgtatactac tattcaacgc attttactat gcgtgctgct agggtcggca
    14761 atgacaatgg caatctgact gacgtggtct atttctccat gtgcagcagg gaatacgagc
    14821 tccaatggac ctcgggagtg gca
//
