LOCUS       sequence-236074-        2327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m.
            Sequence taken from pMAR7 plasmid sequence). Please see Supplemental
            Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2327)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C79m
FEATURES             Location/Qualifiers
     source          1..2327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..66
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(70..113)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    116..137
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    386..406
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(412..429)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(583..1171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(663..682)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1342..2133,2134..2202))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1965..1984
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2203..2307)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
       61 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt
      121 cgcggccgct tctagagact agtgggtctc aaatgatgaa gcagcgtatt acagtgacag
      181 ttgacagcga cagctatcaa ttgctcaagg catatgatgt caatatctcc ggtctggtaa
      241 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gtggaaaatc
      301 aggaagggat ggttgaggtc gcccggttta ttgaaatgaa cggctctttt gccgatgaga
      361 acagggactg gtgaaggtag agacctacta gtagcggccg ctgcaggctt cctcgctcac
      421 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      481 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      541 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc
      601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      721 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      781 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      841 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      901 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      961 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1021 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1081 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1141 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1201 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1261 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1321 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1381 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1441 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgcgacccac gctcaccggc
     1501 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1561 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1621 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1681 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1741 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1801 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1861 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1921 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1981 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2041 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     2101 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2161 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2221 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2281 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgc
//
