LOCUS       sequence-236079-       11901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse PD-L1 3SA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11901)
  AUTHORS   Li CW, Lim SO, Xia W, Lee HH, Chan LC, Kuo CW, Khoo KH, Chang SS, 
            Cha JH, Kim T, Hsu JL, Wu Y, Hsu JM, Yamaguchi H, Ding Q, Wang Y, 
            Yao J, Lee CC, Wu HJ, Sahin AA, Allison JP, Yu D, Hortobagyi GN, 
            Hung MC
  TITLE     Glycosylation and stabilization of programmed death ligand-1 
            suppresses T-cell activity.
  JOURNAL   Nat Commun. 2016 Aug 30;7:12632. doi: 10.1038/ncomms12632.
  PUBMED    27572267
REFERENCE   2  (bases 1 to 11901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-30"; volume: "7:12632. doi"; pages: " 
            10.1038/ncomms12632"
COMMENT     Sequence Label: pGIPz-mPD-L1 3SA
FEATURES             Location/Qualifiers
     source          1..11901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        84..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     542..562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     588..612
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1559..1582
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    1621..2193
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1788..1805)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2015..2034
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2196..2798
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     2695..2715
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     ncRNA           2805..2898
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           2924..3036
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(3131..3149)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3169..3757
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3222..3242)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3640..3651)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3964..4197
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4298..4315)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4304..4528
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4574..5002
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4661..4680)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4871..4892
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5011..5031)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5016..5345
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5196..5331
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5258..5277
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5394..6419
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5394..6419
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6549..6670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6719..6735)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6719..6735)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6732..6754)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6743..6759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6767..6773,6774..6791,6792..6797))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6812..6833
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6950..6967)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7121..7709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7201..7220)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7880..8671,8672..8740))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8503..8522
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8741..8845)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8893..9027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             9195..9828
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9875..10000
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10497..10730
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10915..10959
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11257..11374
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(11439..11813)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      11810..11819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(11823..11841)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(11833..11880)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 ctagacgtat taccgccatg cattagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      601 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
      661 ctctactaga ggatctgcca ccatcgatat gaggatattt gctggcatta tattcacagc
      721 ctgctgtcac ttgctacggg cgtttactat cacggctcca aaggacttgt acgtggtgga
      781 gtatggcagc aacgtcacga tggagtgcag attccctgta gaacgggagc tggacctgct
      841 tgcgttagtg gtgtactggg aaaaggaaga tgagcaagtg attcagtttg tggcaggaga
      901 ggaggacctt aagcctcagc acagcaactt cagggggaga gcctcgctgc caaaggacca
      961 gcttttgaag ggaaatgctg cccttcagat cacagacgtc aagctgcagg acgcaggcgt
     1021 ttactgctgc ataatcagct acggtggtgc ggactacaag cgaatcacgc tgaaagtcaa
     1081 tgccccatac cgcaaaatca accagagaat ttccgtggat ccagccactt ctgagcatga
     1141 actaatatgt caggccgagg gttatccaga agctgaggta atctggacaa acagtgacca
     1201 ccaacccgtg gctgggaaga gagctgtcac cactgcccgg acagagggga tgcttctcaa
     1261 tgtgaccagc agtctgaggg tcaacgccac agcgaatgat gttttctact gtacgttttg
     1321 gagatcacag ccagggcaaa accacacagc ggagctgatc atcccagaac tgcctgcaac
     1381 acatcctcca cagaacagga ctcactgggt gcttctggga tccatcctgt tgttcctcat
     1441 tgtagtgtcc acggtcctcc tcttcttgag aaaacaagtg agaatgctag atgtggagaa
     1501 atgtggcgtt gaagatacaa gctcaaaaaa ccgaaatgat acacaattcg aggagacgga
     1561 ctacaaagac gatgacgaca agtgagctag ctaatgtaca agtagcggcc gcaaattccg
     1621 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     1681 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     1741 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     1801 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     1861 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     1921 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     1981 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     2041 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     2101 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     2161 ggacgtggtt ttcctttgaa aaacacgata ataccatggc caccgagtac aagcccacgg
     2221 tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg
     2281 ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg
     2341 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     2401 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     2461 tcgccgagat cggctcgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     2521 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     2581 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     2641 tggaggcggc cgagcgcgct ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     2701 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     2761 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt ttgtttgaat gaggcttcag
     2821 tactttacag aatcgttgcc tgcacatctt ggaaacactt gctgggatta cttcttcagg
     2881 ttaacccaac agaaggctcg aggtaaccgg atcctgatca gaattcaagg ggctacttta
     2941 ggagcaatta tcttgtttac taaaactgaa taccttgcta tctctttgat acatttttac
     3001 aaagctgaat taaaatggta taaattaaat cacttttttc aattggaaga ctaatgcggc
     3061 cggccattac tccgtctcgt gtcttgttgc atatgtctgc tggtttgttt gatgttgttt
     3121 gcgggcgggc cctatagtga gtcgtattac ctaggacgcg tctggaacaa tcaacctctg
     3181 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3241 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3301 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3361 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3421 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3481 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3541 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     3601 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3661 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3721 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag
     3781 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     3841 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     3901 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg
     3961 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     4021 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     4081 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     4141 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     4201 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4261 gtgagaggcc ttgacattgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     4321 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     4381 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     4441 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     4501 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     4561 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4621 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4681 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4741 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4801 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4861 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4921 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4981 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     5041 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5101 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     5161 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     5221 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     5281 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     5341 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgatgaaaa
     5401 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     5461 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     5521 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     5581 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     5641 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     5701 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     5761 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     5821 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     5881 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     5941 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     6001 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     6061 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     6121 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     6181 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     6241 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     6301 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     6361 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc
     6421 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     6481 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     6541 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6601 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6661 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     6721 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6781 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6841 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6901 gccaacgcgc ggggagaggc ggwttscgya ttrggcgctc ttccgcttcc tcgctcactg
     6961 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7021 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7081 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7141 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7201 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7261 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7321 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7381 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7441 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7501 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7561 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7621 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7681 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7741 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgaaawta taaaaaagga
     7801 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7861 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7921 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7981 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8041 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8101 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8161 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8221 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8281 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8341 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8401 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8461 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8521 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8581 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8641 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8701 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8761 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8821 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     8881 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8941 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9001 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     9061 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     9121 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
     9181 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
     9241 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     9301 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     9361 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     9421 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     9481 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     9541 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     9601 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     9661 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9721 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9781 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     9841 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     9901 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     9961 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
    10021 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
    10081 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
    10141 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
    10201 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
    10261 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
    10321 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
    10381 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
    10441 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
    10501 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
    10561 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
    10621 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
    10681 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
    10741 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
    10801 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
    10861 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
    10921 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
    10981 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
    11041 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
    11101 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
    11161 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
    11221 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
    11281 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
    11341 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
    11401 tccagtttgg ttagtaccgg gcccgctcta gtccggaatc agtcctgctc ctcggccacg
    11461 aagtgcacgc agttgccggc cgggtcgcgc agggcgaact cccgccccca cggctgctcg
    11521 ccgatctcgg tcatggccgg cccggaggcg tcccggaagt tcgtggacac gacctccgac
    11581 cactcggcgt acagctcgtc caggccgcgc acccacaccc aggccagggt gttgtccggc
    11641 accacctggt cctggaccgc gctgatgaac agggtcacgt cgtcccggac cacaccggcg
    11701 aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt cggtccagaa ctcgaccgct
    11761 ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg tcaacttggc catggtggcc
    11821 ctcctatagt gagtcgtatt atactatgcc gatatactat gccgatgatt aattgtcaac
    11881 acgtgctgca ggtccgaggt t
//
