LOCUS       sequence-236087-       16148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer plasmid for expression of SAD B19 L and 
            mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16148)
  AUTHORS   Jin L, Sullivan HA, Zhu M, Lavin TK, Matsuyama M, Fu X, Lea NE, Xu 
            R, Hou Y, Rutigliani L, Pruner M, Babcock KR, Ip JPK, Hu M, Daigle 
            TL, Zeng H, Sur M, Feng G, Wickersham IR
  TITLE     Long-term labeling and imaging of synaptically connected neuronal 
            networks in vivo using double-deletion-mutant rabies viruses.
  JOURNAL   Nat Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8.
  PUBMED    38212587
REFERENCE   2  (bases 1 to 16148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8."
COMMENT     Sequence Label: pLV-TTBL
FEATURES             Location/Qualifiers
     source          1..16148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2550..2568,2569..2591,2592..2610,2611..2633,2634..2652,
                     2653..2675,2676..2694,2695..2717,2718..2736,2737..2759,
                     2760..2778,2779..2801,2802..2820)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     2853..2877
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2963..3583
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             3737..3793
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3794..4492
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4502..4558
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6203..6214
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    10952..11540
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11005..11025)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11423..11434)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11950..12130
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12156..12173)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12162..12386
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12432..12860
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12519..12538)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12729..12750
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12869..12889)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12874..13203
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13054..13189
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13116..13135
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        13251..13298
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             13317..13691
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13821..13942
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13858..13877)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13912..13931
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13991..14007)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13991..14007)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14004..14026)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14015..14031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14039..14045,14046..14063,14064..14069))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14084..14105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14222..14239)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14393..14981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14473..14492)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15152..15943,15944..16012))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15775..15794
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16013..16117)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttat cgttggctcg
     2521 acccgggtac cgagctcgac tttcactttt ctctatcact gatagggagt ggtaaactcg
     2581 actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat
     2641 cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta
     2701 aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
     2761 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
     2821 gtggtaaagg atccctcgac ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2881 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     2941 cgcggccctg caggccgcca ccatgtccag actggacaag agcaaagtca tcaactctgc
     3001 cttggagctc ctgaatgaag ttggcattga gggcttgacc accaggaagc tggcccagaa
     3061 gctgggtgtg gagcagccta ccctgtactg gcatgtgaag aacaagaggg ctctgcttga
     3121 tgccctggcc attgagatgt tggacaggca ccacacccac ttctgccctc tggaagggga
     3181 gtcctggcag gacttcctga ggaacaatgc caagagcttc agatgtgcct tgctctccca
     3241 ccgggatggt gccaaagttc acttgggcac caggcctaca gagaagcagt atgagaccct
     3301 ggagaaccag ctggcattcc tgtgccaaca aggcttctcc ctggaaaatg ccttgtatgc
     3361 cctctctgct gtgggccact tcaccttggg ctgtgtgctg gaggaccagg agcaccaagt
     3421 tgccaaggag gagagggaga cccccaccac tgactccatg ccaccactgc tgcggcaagc
     3481 tattgagttg tttgaccacc aaggggctga gcctgcattc ctttttggcc tggaactgat
     3541 catctgtggc ctggaaaagc agctgaaatg tgagtctggc tctcacgtgc ccaaaaagag
     3601 aaagcacgtg cctgctgatg ccttggatga ttttgacctg gacatgctgc ctgctgatgc
     3661 cctggatgac tttgatttgg acatgctccc tgctgatgca cttgatgatt ttgacctgga
     3721 tatgctggga agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga
     3781 gaaccctgga cctatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga
     3841 gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga
     3901 gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga
     3961 catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat
     4021 ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
     4081 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     4141 ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa
     4201 aacactcggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg
     4261 cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa acgccaagac
     4321 cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga
     4381 ctacagactg gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt
     4441 ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta atggaagcgg
     4501 agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctat
     4561 gctcgatcct ggagaggtct atgatgaccc tattgaccca atcgagttag aggctgaacc
     4621 cagaggaacc cccattgtcc ccaacatctt gaggaactct gactacaatc tcaactctcc
     4681 tttgatagaa gatcctgcta gactaatgtt agaatggtta aaaacaggga atagacctta
     4741 tcggatgact ctaacagaca attgctccag gtctttcaga gttttgaaag attatttcaa
     4801 gaaggtagat ttgggttctc tcaaggtggg cggaatggct gcacagtcaa tgatttctct
     4861 ctggttatat ggtgcccact ctgaatccaa caggagccgg agatgtataa cagacttggc
     4921 ccatttctat tccaagtcgt cccccataga gaagctgttg aatctcacgc taggaaatag
     4981 agggctgaga atccccccag agggagtgtt aagttgcctt gagagggttg attatgataa
     5041 tgcatttgga aggtatcttg ccaacacgta ttcctcttac ttgttcttcc atgtaatcac
     5101 cttatacatg aacgccctag actgggatga agaaaagacc atcctagcat tatggaaaga
     5161 tttaacctca gtggacatcg ggaaggactt ggtaaagttc aaagaccaaa tatggggact
     5221 gctgatcgtg acaaaggact ttgtttactc ccaaagttcc aattgtcttt ttgacagaaa
     5281 ctacacactt atgctaaaag atcttttctt gtctcgcttc aactccttaa tggtcttgct
     5341 ctctccccca gagccccgat actcagatga cttgatatct caactatgcc agctgtacat
     5401 tgctggggat caagtcttgt ctatgtgtgg aaactccggc tatgaagtca tcaaaatatt
     5461 ggagccatat gtcgtgaata gtttagtcca gagagcagaa aagtttaggc ctctcattca
     5521 ttccttggga gactttcctg tatttataaa agacaaggta agtcaacttg aagagacgtt
     5581 cggtccctgt gcaagaaggt tctttagggc tctggatcaa ttcgacaaca tacatgactt
     5641 ggtttttgtg tttggctgtt acaggcattg ggggcaccca tatatagatt atcgaaaggg
     5701 tctgtcaaaa ctatatgatc aggttcacct taaaaaaatg atagataagt cctaccagga
     5761 gtgcttagca agcgacctag ccaggaggat ccttagatgg ggttttgata agtactccaa
     5821 gtggtatctg gattcaagat tcctagcccg agaccacccc ttgactcctt atatcaaaac
     5881 ccaaacatgg ccacccaaac atattgtaga cttggtgggg gatacatggc acaagctccc
     5941 gatcacgcag atctttgaga ttcctgaatc aatggatccg tcagaaatat tggatgacaa
     6001 atcacattct ttcaccagaa cgagactagc ttcttggctg tcagaaaacc gaggggggcc
     6061 tgttcctagc gaaaaagtta ttatcacggc cctgtctaag ccgcctgtca atccccgaga
     6121 gtttctgagg tctatagacc tcggaggatt gccagatgaa gacttgataa ttggcctcaa
     6181 gccaaaggaa cgggaattga agattgaagg tcgattcttt gctctaatgt catggaatct
     6241 aagattgtat tttgtcatca ctgaaaaact cttggccaac tacatcttgc cactttttga
     6301 cgcgctgact atgacagaca acctgaacaa ggtgtttaaa aagctgatcg acagggtcac
     6361 cgggcaaggg cttttggact attcaagggt cacatatgca tttcacctgg actatgaaaa
     6421 gtggaacaac catcaaagat tagagtcaac agaggatgta ttttctgtcc tagatcaagt
     6481 gtttggattg aagagagtgt tttctagaac acacgagttt tttcaaaagg cctggatcta
     6541 ttattcagac agatcagacc tcatcgggtt acgggaggat caaatatact gcttagatgc
     6601 gtccaacggc ccaacctgtt ggaatggcca ggatggcggg ctagaaggct tacggcagaa
     6661 gggctggagt ctagtcagct tattgatgat agatagagaa tctcaaatca ggaacacaag
     6721 aaccaaaata ctagctcaag gagacaacca ggttttatgt ccgacataca tgttgtcgcc
     6781 agggctatct caagaggggc tcctctatga attggagaga atatcaagga atgcactttc
     6841 gatatacaga gccgtcgagg aaggggcatc taagctaggg ctgatcatca agaaagaaga
     6901 gaccatgtgt agttatgact tcctcatcta tggaaaaacc cctttgttta gaggtaacat
     6961 attggtgcct gagtccaaaa gatgggccag agtctcttgc gtctctaatg accaaatagt
     7021 caacctcgcc aatataatgt cgacagtgtc caccaatgcg ctaacagtgg cacaacactc
     7081 tcaatctttg atcaaaccga tgagggattt tctgctcatg tcagtacagg cagtctttca
     7141 ctacctgcta tttagcccaa tcttaaaggg aagagtttac aagattctga gcgctgaagg
     7201 ggagagcttt ctcctagcca tgtcaaggat aatctatcta gatccttctt tgggagggat
     7261 atctggaatg tccctcggaa gattccatat acgacagttc tcagaccctg tctctgaagg
     7321 gttatccttc tggagagaga tctggttaag ctcccaagag tcctggattc acgcgttgtg
     7381 tcaagaggct ggaaacccag atcttggaga gagaacactc gagagcttca ctcgccttct
     7441 agaagatccg accaccttaa atatcagagg aggggccagt cctaccattc tactcaagga
     7501 tgcaatcaga aaggctttat atgacgaggt ggacaaggtg gaaaattcag agtttcgaga
     7561 ggcaatcctg ttgtccaaga cccatagaga taattttata ctcttcttaa tatctgttga
     7621 gcctctgttt cctcgatttc tcagtgagct attcagttcg tcttttttgg gaatccccga
     7681 gtcaatcatt ggattgatac aaaactcccg aacgataaga aggcagttta gaaagagtct
     7741 ctcaaaaact ttagaagaat ccttctacaa ctcagagatc cacgggatta gtcggatgac
     7801 ccagacacct cagagggttg ggggggtgtg gccttgctct tcagagaggg cagatctact
     7861 tagggagatc tcttggggaa gaaaagtggt aggcacgaca gttcctcacc cttctgagat
     7921 gttgggatta cttcccaagt cctctatttc ttgcacttgt ggagcaacag gaggaggcaa
     7981 tcctagagtt tctgtatcag tactcccgtc ctttgatcag tcattttttt cacgaggccc
     8041 cctaaaggga tacttgggct cgtccacctc tatgtcgacc cagctattcc atgcatggga
     8101 aaaagtcact aatgttcatg tggtgaagag agctctatcg ttaaaagaat ctataaactg
     8161 gttcattact agagattcca acttggctca agctctaatt aggaacatta tgtctctgac
     8221 aggccctgat ttccctctag aggaggcccc tgtcttcaaa aggacggggt cagccttgca
     8281 taggttcaag tctgccagat acagcgaagg agggtattct tctgtctgcc cgaacctcct
     8341 ctctcatatt tctgttagta cagacaccat gtctgatttg acccaagacg ggaagaacta
     8401 cgatttcatg ttccagccat tgatgcttta tgcacagaca tggacatcag agctggtaca
     8461 gagagacaca aggctaagag actctacgtt tcattggcac ctccgatgca acaggtgtgt
     8521 gagacccatt gacgacgtga ccctggagac ctctcagatc ttcgagtttc cggatgtgtc
     8581 gaaaagaata tccagaatgg tttctggggc tgtgcctcac ttccagaggc ttcccgatat
     8641 ccgtctgaga ccaggagatt ttgaatctct aagcggtaga gaaaagtctc accatatcgg
     8701 atcagctcag gggctcttat actcaatctt agtggcaatt cacgactcag gatacaatga
     8761 tggaaccatc ttccctgtca acatatacgg caaggtttcc cctagagact atttgagagg
     8821 gctcgcaagg ggagtattga taggatcctc gatttgcttc ttgacaagaa tgacaaatat
     8881 caatattaat agacctcttg aattggtctc aggggtaatc tcatatattc tcctgaggct
     8941 agataaccat ccctccttgt acataatgct cagagaaccg tctcttagag gagagatatt
     9001 ttctatccct cagaaaatcc ccgccgctta tccaaccact atgaaagaag gcaacagatc
     9061 aatcttgtgt tatctccaac atgtgctacg ctatgagcga gagataatca cggcgtctcc
     9121 agagaatgac tggctatgga tcttttcaga ctttagaagt gccaaaatga cgtacctatc
     9181 cctcattact taccagtctc atcttctact ccagagggtt gagagaaacc tatctaagag
     9241 tatgagagat aacctgcgac aattgagttc tttgatgagg caggtgctgg gcgggcacgg
     9301 agaagatacc ttagagtcag acgacaacat tcaacgactg ctaaaagact ctttacgaag
     9361 gacaagatgg gtggatcaag aggtgcgcca tgcagctaga accatgactg gagattacag
     9421 ccccaacaag aaggtgtccc gtaaggtagg atgttcagaa tgggtctgct ctgctcaaca
     9481 ggttgcagtc tctacctcag caaacccggc ccctgtctcg gagcttgaca taagggccct
     9541 ctctaagagg ttccagaacc ctttgatctc gggcttgaga gtggttcagt gggcaaccgg
     9601 tgctcattat aagcttaagc ctattctaga tgatctcaat gttttcccat ctctctgcct
     9661 tgtagttggg gacgggtcag gggggatatc aagggcagtc ctcaacatgt ttccagatgc
     9721 caagcttgtg ttcaacagtc ttttagaggt gaatgacctg atggcttccg gaacacatcc
     9781 actgcctcct tcagcaatca tgaggggagg aaatgatatc gtctccagag tgatagatct
     9841 tgactcaatc tgggaaaaac cgtccgactt gagaaacttg gcaacctgga aatacttcca
     9901 gtcagtccaa aagcaggtca acatgtccta tgacctcatt atttgcgatg cagaagttac
     9961 tgacattgca tctatcaacc ggatcaccct gttaatgtcc gattttgcat tgtctataga
    10021 tggaccactc tatttggtct tcaaaactta tgggactatg ctagtaaatc caaactacaa
    10081 ggctattcaa cacctgtcaa gagcgttccc ctcggtcaca gggtttatca cccaagtaac
    10141 ttcgtctttt tcatctgagc tctacctccg attctccaaa cgagggaagt ttttcagaga
    10201 tgctgagtac ttgacctctt ccacccttcg agaaatgagc cttgtgttat tcaattgtag
    10261 cagccccaag agtgagatgc agagagctcg ttccttgaac tatcaggatc ttgtgagagg
    10321 atttcctgaa gaaatcatat caaatcctta caatgagatg atcataactc tgattgacag
    10381 tgatgtagaa tcttttctag tccacaagat ggttgatgat cttgagttac agaggggaac
    10441 tctgtctaaa gtggctatca ttatagccat catgatagtt ttctccaaca gagtcttcaa
    10501 cgtttccaaa cccctaactg acccctcgtt ctatccaccg tctgatccca aaatcctgag
    10561 gcacttcaac atatgttgca gtactatgat gtatctatct actgctttag gtgacgtccc
    10621 tagcttcgca agacttcacg acctgtataa cagacctata acttattact tcagaaagca
    10681 agtcattcga gggaacgttt atctatcttg gagttggtcc aacgacacct cagtgttcaa
    10741 aagggtagcc tgtaattcta gcctgagtct gtcatctcac tggatcaggt tgatttacaa
    10801 gatagtgaag actaccagac tcgttggcag catcaaggat ctatccagag aagtggaaag
    10861 acaccttcat aggtacaaca ggtggatcac cctagaggat atcagatcta gatcatccct
    10921 actagactac agttgcctgt gatgagctag caatcaacct ctggattaca aaatttgtga
    10981 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
    11041 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
    11101 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
    11161 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
    11221 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
    11281 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
    11341 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
    11401 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
    11461 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
    11521 cctttgggcc gcctccccgc ctgttcgaat tcgagctcgg tacctttaag accaatgact
    11581 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
    11641 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
    11701 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
    11761 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
    11821 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
    11881 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
    11941 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
    12001 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
    12061 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
    12121 atctctagca gggccgcttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
    12181 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
    12241 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
    12301 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
    12361 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
    12421 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
    12481 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
    12541 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
    12601 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
    12661 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
    12721 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
    12781 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
    12841 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
    12901 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
    12961 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
    13021 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
    13081 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    13141 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
    13201 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
    13261 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
    13321 ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt
    13381 tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg
    13441 tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca
    13501 ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg
    13561 tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt
    13621 gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg
    13681 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
    13741 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
    13801 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
    13861 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    13921 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
    13981 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
    14041 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
    14101 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
    14161 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
    14221 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    14281 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    14341 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    14401 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    14461 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    14521 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    14581 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    14641 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    14701 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    14761 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    14821 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    14881 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    14941 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    15001 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    15061 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    15121 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    15181 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    15241 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    15301 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    15361 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    15421 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    15481 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    15541 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    15601 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    15661 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    15721 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    15781 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    15841 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    15901 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    15961 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    16021 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    16081 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    16141 ctgacgtc
//
