LOCUS       sequence-236095-        4469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for mfLon protease (see doi: 
            [10.1038/nbt.3053]). Please see Supplemental Documents for annotated
            Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4469)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C80m
FEATURES             Location/Qualifiers
     source          1..4469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     58..76
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(80..123)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    126..147
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    2538..2558
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(2564..2581)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2735..3323)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2815..2834)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3494..4285,4286..4354))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4117..4136
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4355..4459)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
       61 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat
      121 ttctggaatt cgcggccgct tctagagact agtgggtctc aaatgtctaa aaaaatcaaa
      181 ctgccgatct tccagatccg tggttctttc atcgttccgg gtatcaaaga aaacctggaa
      241 gttggtcgta aaaacaccct ggcgtctgtt aactacgcga tcaaaaactc taacaaccag
      301 atgatcgcga tcccgcagat cgacgcgtct gttgaaaaac cggaattctc tgacctgcac
      361 gaattcggta tcctgatcga cttcgaagtt atcaaagaat ggaaagacaa ctctctgacc
      421 atctctacca acccgatcca gcgttgcaaa gttatctctt tcttcgaaaa cgaagatcag
      481 gttccgtacg cggaagttga actgatcgaa tctatcaacg acttctctga cgaagaactg
      541 aaagaactga tcgaaaaaat ctctgacgcg atcaaaacca aagcgtctct ggttaccaaa
      601 cagatcaaac agctgatctc tggtgaatct gacgacctgt ctctggcgtt cgactctatc
      661 atgttcaaac tggcgccgtc taaaatcctg accaacccgg aatacatcac ctctccgtct
      721 ctgaaaaccc gttggtctat catcgaaaaa atcatcttcg cggaagatgg tatcatcacc
      781 cgtaacgcgg aatctatcga cgcggcgcgt cagaaaaacg aaatcgaaca ggaactgaac
      841 cacaaactga aagaaaaaat ggacaaacag cagaaagaat actacctgcg tgaaaaaatg
      901 cgtatcatca aagacgaact ggaagatgaa gatgactctg acgactcttc tctggaaaaa
      961 tacaaagaac gtctggcgaa agaaccgttc ccggaagaag ttaaacgtaa aatcatggcg
     1021 tctatcaaac gtgttgaagc gctgcagtct ggtaccccgg aatggaacac cgaaaaaaac
     1081 tacatcgact ggatgatgtc tatcccgtgg tgggaagaaa ccgaagatct gaccgacctg
     1141 aaatacgcga aaaaaatcct ggacaaacac cactacggta tgaaaaaagt taaagaacgt
     1201 atcatcgaat acctggcggt taaaaccaaa accaaatctc tgaaagcgcc gatcatcacc
     1261 ctggttggtc cgccgggtgt tggtaaaacc tctctggcga aatctatcgc ggaagcggtt
     1321 ggtaaaaact tcgttaaagt ttctctgggt ggtgttaaag acgaatctga aatccgtggt
     1381 caccgtaaaa cctacgttgg ttctatgccg ggtcgtatca tccagaccat gaaacgtgcg
     1441 aaagttaaaa acccgctgtt cctgctggac gaaatcgaca aaatggcgtc tgaccaccgt
     1501 ggtgacccgg cgtctgcgat gctggaagtt ctggacccgg aacagaacaa agaattctct
     1561 gaccactaca tcgaagaacc gtacgacctg tctcaggtta tgttcatcgc gaccgcgaac
     1621 tacccggaag atatcccgga agcgctgtac gaccgtatgg aaatcatcaa cctgtcctct
     1681 tacaccgaaa tcgaaaaagt taaaatcgcg caggactacc tggttccgaa agcgatcgaa
     1741 cagcacgaac tgacctctga agaaatctct ttcaccgaag gtgcgatcaa cgaaatcatc
     1801 aaatactaca cccgtgaagc gggtgttcgt cagctggaac gtcacatcaa ctctatcatc
     1861 cgtaaataca tcgttaaaaa cctgaacggt gaaatggaca aaatcgttat cgacgaaaaa
     1921 caggttaacg acctgctggg taaacgtatc ttcgaccaca ccgaaaaaca ggaagaatct
     1981 cagatcggtg ttgttaccgg tctggcgtac acccagttcg gtggtgacat cctgccgatc
     2041 gaagtttctc tgtacccggg taaaggtaac ctgatcctga ccggtaaact gggtgaagtt
     2101 atgaaagaat ctgcgaccat cgcgctgacc tacgttaaat ctaacttcga aaaattcggt
     2161 gttgacaaaa aagttttcga agaaaacgac atccacgttc acgttccgga aggtgcggtt
     2221 ccgaaagacg gtccgtctgc gggtatcacc atcaccaccg cgctgatctc tgcgctgtct
     2281 gacaaaccgg tttctaaaga aatcggtatg accggtgaaa tcaccctgcg tggtaacgtt
     2341 ctgccgatcg gtggtctgcg tgaaaaatct atctctgcgt ctcgttctgg tctgaaaacc
     2401 atcatcatcc cgaaaaaaaa cgaacgtgac ctggacgaaa tcccggacga agttaaagcg
     2461 aaactgaaaa tcatcccggc ggaaaaatac gaagaagttt tcgcgatcgt tttcaaaacc
     2521 aaataaaggt agagacctac tagtagcggc cgctgcaggc ttcctcgctc actgactcgc
     2581 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2641 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2701 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg
     2761 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2821 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2881 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2941 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3001 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3061 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3121 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3181 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3241 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3301 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3361 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3421 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3481 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3541 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3601 taccatctgg ccccagtgct gcaatgatac cgcgcgaccc acgctcaccg gctccagatt
     3661 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3721 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3781 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3841 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3901 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3961 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4021 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4081 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4141 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4201 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4261 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4321 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4381 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4441 aacaaatagg ggttccgcgc acatttccc
//
