LOCUS       sequence-236098-        2762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for RpaI (synthase for 
            Rpa-detected quorum sensing molecule 4-coumaroyl-homoserine 
            lactone). Please see Supplemental Documents for annotated Genbank 
            file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2762)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C82m
FEATURES             Location/Qualifiers
     source          1..2762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..45
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(49..92)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    95..116
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    800..820
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(826..843)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(997..1585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1077..1096)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1756..2547,2548..2616))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2379..2398
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2617..2721)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
       61 aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagacta
      121 gtgggtctca aatgcaggtt catgtcatcc gtcgagagaa ccgcgcgctc tatgccggtc
      181 tgctcgaaaa gtacttccgc atccgtcacc agatctacgt cgtcgagcgc ggctggaagg
      241 agctcgatcg gccggatggc cgcgagatcg atcagttcga caccgaagac gccgtgtatc
      301 tgctcggcgt cgacaatgac gacatcgtcg ccggcatgcg gatggtgccg accacgtcac
      361 cgacgctcct cagcgacgtc ttcccgcagc ttgcgctggc aggcccggtg cggcggccgg
      421 atgcctacga gctgtcgcgg atcttcgtgg taccgcgcaa gcgcggcgag catggcggcc
      481 cgcgcgccga agccgtgatc caggcggccg cgatggagta cggcctgtcg atcggtctgt
      541 cggccttcac catcgtgctg gagacctggt ggctgccgcg actggtggac cagggctgga
      601 aggcaaagcc gctcggcctg cctcaggaca tcaacggatt ctcgaccacc gcagtgatcg
      661 tcgacgtcga cgacgacgcc tgggtcggca tctgcaatcg ccgctcggtg cccggaccca
      721 cgctggaatg gcgcgggctc gaagccatcc gccgtcattc gcttccggaa ttccaggtga
      781 tttcataaag gtagagacct actagtagcg gccgctgcag gcttcctcgc tcactgactc
      841 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      901 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga
     1021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1501 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1561 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1621 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1681 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1741 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1801 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1861 cttaccatct ggccccagtg ctgcaatgat accgcgcgac ccacgctcac cggctccaga
     1921 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1981 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2041 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2101 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2161 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2221 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2281 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2341 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2401 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2461 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2521 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     2581 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     2641 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     2701 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     2761 ca
//
