LOCUS       sequence-236113-        8751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  orange calcium-modulated bioluminescent indicator, 300nM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8751)
  AUTHORS   Oh Y, Park Y, Cho JH, Wu H, Paulk NK, Liu LX, Kim N, Kay MA, Wu JC, 
            Lin MZ
  TITLE     An orange calcium-modulated bioluminescent indicator for 
            non-invasive activity imaging.
  JOURNAL   Nat Chem Biol. 2019 Apr 1. pii: 10.1038/s41589-019-0256-z. doi: 
            10.1038/s41589-019-0256-z.
  PUBMED    30936501
REFERENCE   2  (bases 1 to 8751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2019 Apr 1. pii: 10.1038/s41589-019-0256-z. doi: 
            10.1038/s41589-019-0256-z."
COMMENT     Sequence Label: pcDNA3.1-Orange_CaMBI_300
FEATURES             Location/Qualifiers
     source          1..8751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        92..395
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        397..670
                     /label=chicken beta-actin promoter
     intron          671..1687
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1611..1633
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1695..1714
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1777..1786
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3313..3390
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             4255..4284
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4300..4317
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4340..4357)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4346..4570
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4616..5044
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4703..4722)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4913..4934
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5053..5073)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5058..5387
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5238..5373
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5300..5319
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5454..6248
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5508..5527)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6118..6137
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6424..6545
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6461..6480)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6515..6534
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6594..6610)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6594..6610)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6607..6629)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6618..6634
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6642..6648,6649..6666,6667..6672))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6687..6708
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6825..6842)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6996..7584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7076..7095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7755..8546,8547..8615))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8378..8397
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8616..8720)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcaatt ctgcaggaat ctagttatta atagtaatca attacggggt
       61 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      121 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      181 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      241 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      301 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      361 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
      421 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
      481 tattttgtgc agcgatgggg gcgggggggg ggggcgcgcg ccaggcgggg cggggcgggg
      541 cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct
      601 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
      661 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
      721 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
      781 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
      841 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
      901 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
      961 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
     1021 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
     1081 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     1141 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     1201 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     1261 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg
     1321 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     1381 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     1441 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     1501 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     1561 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     1621 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     1681 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     1741 gatttgatac cgcgggcccg ggatcagctt ggtaccgcca ccatggtgag caagggcgag
     1801 gagctgatca aggagaacat gagaagcaag ctgtacctgg aaggcagcgt gaacggccac
     1861 cagttcaagt gcacccacga aggggagggc aagccctacg agggcaagca gaccaacagg
     1921 atcaaggtgg tggagggagg ccccctgccg ttcgcattcg acatcctggc cacccacttt
     1981 atgtacggga gcaaggtgtt catcaagtac cccgccgacc tccccgatta ttttaagcag
     2041 tccttccctg agggcttcac atgggagaga gtcatggtgt tcgaagacgg gggcgtgctg
     2101 accgccaccc aggacaccag cctccaggac ggcgagctca tctacaacgt caaggtcaga
     2161 ggggtgaact tcccagccaa cggccccgtg atgcagaaga aaacactggg ctgggagccc
     2221 agcaccgaga ccatgtaccc cgctgacggc ggcctggaag gcagatgcga caaggccctg
     2281 aagctcgtgg gcgggggcca cctgcacgtc aacttcaaga ccacatacaa gtccaagaaa
     2341 cccgtgaaga tgcccggcgt ccactacgtg gaccgcagac tggaaagaat caaggaggcc
     2401 gacaacgaga cctacgtcga gcagtacgag cacgctgtgg ccagatactc caacctgggc
     2461 ggaggcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg
     2521 gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta
     2581 actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc
     2641 atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag
     2701 gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta
     2761 atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc
     2821 gtgttcgacg gcaaaaagat cactgtaaca gggaccctga ttatgcatga ccaactgaca
     2881 gaagagcaga ttgcagagtt caaagaagcc ttctcattat tcgacaagga tggggacggc
     2941 accatcacca caaaggaact tggcaccgtt atgaggtcgc ttggacaaaa cccaacggaa
     3001 gcagaattgc aggatatgat caatgaagtc gatgctgatg gcaatggaac gatttacttt
     3061 cctgaatttc ttactatgat ggctagaaaa atgaaggaca cagacagcga agaggaaatc
     3121 cgagaagcat tccgtgtttt tgacaaggat gggaacggct acatcagcgc tgctcagtta
     3181 cgtcacgtca tgacaaacct cggggagaag ttaacagatg aagaagttga tgaaatgata
     3241 agggaagcag atatcgatgg tgatggccaa gtaaactatg aagagtttgt acaaatgatg
     3301 acagcaaagg gtaagaggcg ctggaagaaa aacttcattg ccgtcagcgc tgccaaccgg
     3361 ttcaagaaga tctccagctc cggggcactg gagctctgga acggcaacaa aattatcgac
     3421 gagcgcctga tcaaccccga cggctccctg ctgttccgag taaccatcaa cggagtgacc
     3481 ggctggcggc tgtgcgaacg cattctggcg aggcacgaac tcattaaaga aaatatgcgg
     3541 tccaagctct atctcgaggg aagtgtgaat ggtcatcaat ttaagtgtac tcatgagggc
     3601 gaaggtaaac cttacgaagg aaagcaaacg aatcgaataa aggtcgtgga aggcggaccg
     3661 ctcccttttg ccttcgatat tctggctaca catttcatgt atggatctaa agtctttatt
     3721 aagtatcctg cggatctgcc agactacttt aagcagagct ttccagaagg atttacttgg
     3781 gaacgagtga tggtctttga ggatggaggt gtcctcacag ctacgcaaga tacttccctt
     3841 caagatggag aactgattta caacgtgaaa gtgcgcggtg tcaattttcc tgcaaatggt
     3901 ccagtcatgc aaaagaagac cctcggatgg gaaccttcta cagaaacaat gtatcctgca
     3961 gatggaggtc tcgagggtcg gtgtgataag gctcttaaac tggtcggagg aggtcatctc
     4021 catgtgaatt tcaaaactac ctataaaagc aaaaagcctg tcaaaatgcc aggtgtacat
     4081 tatgtcgatc gacggctcga gcggataaag gaagctgata atgaaacgta tgtggaacag
     4141 tatgaacatg ccgtcgcacg gtatagtaat ctcggtggcg gaatggatga actctataag
     4201 taatgaattc tgcagatatc cagcacagtg gcggccgctc gaggtcaccc attcgaacaa
     4261 aaactcatct cagaagagga tctgaatatg cataccggtc atcatcacca tcaccattga
     4321 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     4381 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     4441 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     4501 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     4561 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     4621 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4681 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4741 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4801 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4861 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4921 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4981 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5041 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     5101 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     5161 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     5221 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5281 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     5341 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     5401 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     5461 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     5521 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     5581 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     5641 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     5701 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     5761 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     5821 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     5881 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     5941 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     6001 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     6061 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     6121 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     6181 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     6241 tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca
     6301 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6361 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6421 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6481 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6541 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     6601 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6661 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6721 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6781 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6841 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6901 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6961 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7021 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7081 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7141 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7201 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7261 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7321 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7381 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7441 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7501 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7561 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7621 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7681 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7741 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7801 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7861 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7921 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7981 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8041 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8101 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8161 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8221 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8281 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8341 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8401 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8461 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8521 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8581 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8641 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8701 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
