LOCUS       sequence-236125-        2690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA
            polymerase involved in heat response and biofilm formation). Please 
            see Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2690)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C85m
FEATURES             Location/Qualifiers
     source          1..2690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3..21
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(25..68)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    71..92
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    704..724
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(730..747)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(901..1489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(981..1000)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1660..2451,2452..2520))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2283..2302
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2521..2625)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
       61 atgatttctg gaattcgcgg ccgcttctag agactagtgg gtctcaaatg atgaatgaaa
      121 ccgatcctga tctggaactg ctgaaacgta ttggtaataa tgatgcacag gccgttaaag
      181 aaatggttac ccgtaaactg cctcgtctgc tggcactggc aagtcgcctg ctgggtgatg
      241 cagatgaagc acgtgatatt gcacaagaaa gttttctgcg catttggaaa caggcagcaa
      301 gctggcgtag cgaacaggca cgttttgata cctggctgca tcgtgttgca ctgaatctgt
      361 gttatgatcg tctgcgtcgt cgtaaagaac atgtgccggt tgatagcgaa catgcctgtg
      421 aagcactgga tacccgtccg gcaccggatg aacagctgga agcaagcgca cagagccgtc
      481 gtatggcaca ggcactggat cagctgccgg atcgtcagcg tgaagcaatt gttctgcagt
      541 attatcaaga actgagcaat accgaagcag cagcactgat gcaaattagc gttgaagccc
      601 tggaaagcct gctgagccgt gcacgtcgta atctgcgtag ccatctggcc gaagcaccgg
      661 gtgcagatct gagcggtcgt cgcaaaccgt aaaggtagag acctactagt agcggccgct
      721 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      781 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      841 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      901 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      961 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1021 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1081 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1141 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1201 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1261 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1321 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1381 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1441 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1501 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1561 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1621 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1681 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1741 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1801 cgacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1861 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1921 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1981 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2041 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2101 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2161 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2221 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2281 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2341 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2401 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     2461 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     2521 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2581 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2641 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
//
