LOCUS       sequence-236126-        2381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part (phage lysis protein; lethal to 
            host when expressed). Please see Supplemental Documents for 
            annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2381)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C86m
FEATURES             Location/Qualifiers
     source          1..2381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     33..51
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(55..98)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    101..122
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    425..445
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(451..468)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(622..1210)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(702..721)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1381..2172,2173..2241))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2004..2023
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2242..2346)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca
       61 gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag
      121 agactagtgg gtctcaaatg gtacgctgga ctttgtggga taccctcgct ttcctgctcc
      181 tgttgagttt attgctgccg tcattgctta ttatgttcat cccgtcaaca ttcaaacggc
      241 ctgtctcatc atggaaggcg ctgaatttac ggaaaacatt attaatggcg tcgagcgtcc
      301 ggttaaagcc gctgaattgt tcgcgtttac cttgcgtgta cgcgcaggaa acactgacgt
      361 tcttactgac gcagaagaaa acgtgcgtca aaaattacgt gcggaaggag taaaggtaga
      421 gacctactag tagcggccgc tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt
      481 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      541 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      601 aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa
      661 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      721 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      781 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      841 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      901 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      961 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1021 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1081 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1141 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1201 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1261 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1321 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1381 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1441 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1501 cagtgctgca atgataccgc gcgacccacg ctcaccggct ccagatttat cagcaataaa
     1561 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1621 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1681 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1741 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     1801 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     1861 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     1921 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     1981 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     2041 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     2101 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     2161 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     2221 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     2281 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2341 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa g
//
