LOCUS       sequence-236128-        3746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for NahR-sfGFP fusion (Adam Meyer
            improved NahR (C75m) fused to superfolder GFP (C3m)). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3746)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C90m
FEATURES             Location/Qualifiers
     source          1..3746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     44..62
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(66..109)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    112..133
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             1073..1786
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    1801..1821
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1827..1844)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1998..2586)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2078..2097)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2757..3548,3549..3617))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3380..3399
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3618..3722)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
       61 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg
      121 gccgcttcta gagactagtg ggtctcaaat ggaactgcgt gacctggatt taaacctgct
      181 ggtggtgttc aaccagttgc tggtcgacag acgcgtctct gtcactgcgg agaacctggg
      241 cctgacccag cctgccgtga gcaatgcgct gaaacgcctg cgcacctcgc tacaggaccc
      301 actcttcgtg cgcacacatc agggaatgga acccacaccc tatgccgcgc atctggccga
      361 gcacgtcact tcggccatgc acgcactgcg caacgcccta cagcaccatg aaagcttcga
      421 tccgctgacc agcgagcgta ccttcaccct ggccatgacc gacattggcg agatctactt
      481 catgccgcgg ctgatggatg cgctggctca ccaggccccc aattgcgtga tcagtacggt
      541 gcgcgacagt tcgatgagcc tgatgcaggc cttgcagaac ggaaccgtgg acttggccgt
      601 gggcctgctt cccaatctgc aaactggctt ctttcagcgc cggctgctcc agaatcacta
      661 cgtgtgccta tgtcgcaagg accatccagt cacccgcgaa cccctgactc tggagcgctt
      721 ctgttcctac ggccacgtgc gtgtcatcgc cgctggcacc ggccacggcg aggtggacac
      781 gtacatgaca cgggtcggca tccggcgcga catccgtctg gaagtgccgc acttcgccgc
      841 cgttggccac atcctccagc gcaccgatct gctcgccact gtgccgatat gtttagccga
      901 ctgctgcgta gagcccttcg gcctaagcgc cttgccgcac ccagtcgtct tgcctgaaat
      961 agccatcaac atgttctggc atgcgaagta ccacaaggac ctagccaata tttggttgcg
     1021 gcaactgatg tttgacctgt ttacggatgg aagcggaagt ggatccggta gtatgcgtaa
     1081 aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa
     1141 cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac
     1201 gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac
     1261 gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt
     1321 cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga
     1381 cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat
     1441 tgagctgaaa ggcattgact ttaaagaaga tggcaatatc ctgggccata agctggaata
     1501 caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc
     1561 gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca
     1621 gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac
     1681 gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt
     1741 cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaataaa ggtagagacc
     1801 tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1861 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1921 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1981 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
     2041 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2101 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2161 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2221 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2281 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2341 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2401 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2461 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2521 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2581 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2641 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2701 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2761 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2821 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2881 gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc aataaaccag
     2941 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3001 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3061 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3121 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3181 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3241 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3301 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3361 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3421 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3481 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3541 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3601 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3661 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3721 cgcacatttc cccgaaaagt gccacc
//
