LOCUS       sequence-236129-        2822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for sfCFP (superfolder CFP 
            developed by Ye Chen in Matthew Bennet's lab) - Please see 
            Supplemental Documents for annotated Genbank file.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2822)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C91m
FEATURES             Location/Qualifiers
     source          1..2822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..45
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(49..92)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    95..116
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             132..845
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    860..880
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(886..903)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1057..1645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1137..1156)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1816..2607,2608..2676))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2439..2458
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2677..2781)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
       61 aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagacta
      121 gtgggtctca aatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct attctggtgg
      181 aactggatgg tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg
      241 caactaatgg taaactgacg ctgaagttca tctgtactac tggtaaactg ccggtacctt
      301 ggccgactct ggtaacgacg ctgacttggg gtgttcagtg ctttgctcgt tatccggacc
      361 atatgaagca gcatgacttc ttcaagtccg ccatgccgga aggctatgtg caggaacgca
      421 cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg
      481 ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaagac ggcaatatcc
      541 tgggccataa gctggaatac aattacatca gcgacaatgt ttacatcacc gccgataaac
      601 aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc
      661 agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt ctgctgccag
      721 acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc
      781 atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg gatgaactgt
      841 acaaataaag gtagagacct actagtagcg gccgctgcag gcttcctcgc tcactgactc
      901 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      961 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1021 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga
     1081 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1141 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1201 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1261 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1321 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1381 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1441 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1501 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1561 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1621 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1681 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1741 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1801 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1861 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1921 cttaccatct ggccccagtg ctgcaatgat accgcgcgac ccacgctcac cggctccaga
     1981 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2041 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2101 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2161 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2221 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2281 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2341 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2401 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2461 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2521 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2581 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     2641 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     2701 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     2761 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     2821 ca
//
