LOCUS       sequence-236130-        4760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for T7 RNA polymerase (use with 
            P18m). Please see Supplemental Documents for annotated Genbank 
            file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4760)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C95m
FEATURES             Location/Qualifiers
     source          1..4760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(17..60)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    63..84
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    2766..2786
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(2792..2809)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2963..3551)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3043..3062)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3722..4513,4514..4582))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4345..4364
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4583..4687)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(4755..4760,1..13)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
       61 tggaattcgc ggccgcttct agagactagt gggtctcaaa tgaacacgat taacatcgct
      121 aagaacgact tctctgacat cgaactggct gctatcccgt tcaacactct ggctgaccat
      181 tacggtgagc gtttagctcg cgaacagttg gcccttgagc atgagtctta cgagatgggt
      241 gaagcacgct tccgcaagat gtttgagcgt caacttaaag ctggtgaggt tgcggataac
      301 gctgccgcca agcctctcat cactacccta ctccctaaga tgattgcacg catcaacgac
      361 tggtttgagg aagtgaaagc taagcgcggc aagcgcccga cagccttcca gttcctgcaa
      421 gaaatcaagc cggaagccgt agcgtacatc accattaaga ccactctggc ttgcctaacc
      481 agtgctgaca atacaaccgt tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac
      541 gaggctcgct tcggtcgtat ccgtgacctt gaagctaagc acttcaagaa aaacgttgag
      601 gaacaactca acaagcgcgt agggcacgtc tacaagaaag catttatgca agttgtcgag
      661 gctgacatgc tctctaaggg tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa
      721 gactctattc atgtaggagt acgctgcatc gagatgctca ttgagtcaac cggaatggtt
      781 agcttacacc gccaaaatgc tggcgtagta ggtcaagact ctgagactat cgaactcgca
      841 cctgaatacg ctgaggctat cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg
      901 ttccaacctt gcgtagttcc tcctaagccg tggactggca ttactggtgg tggctattgg
      961 gctaacggtc gtcgtcctct ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc
     1021 tacgaagacg tttacatgcc tgaggtgtac aaagcgatta acattgcgca aaacaccgca
     1081 tggaaaatca acaagaaagt cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt
     1141 ccggtcgagg acatccctgc gattgagcgt gaagaactcc cgatgaaacc ggaagacatc
     1201 gacatgaatc ctgaggctct caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag
     1261 gacaaggctc gcaagtctcg ccgtatcagc cttgagttca tgcttgagca agccaataag
     1321 tttgctaacc ataaggccat ctggttccct tacaacatgg actggcgcgg tcgtgtttac
     1381 gctgtgtcaa tgttcaaccc gcaaggtaac gatatgacca aaggactgct tacgctggcg
     1441 aaaggtaaac caatcggtaa ggaaggttac tactggctga aaatccacgg tgcaaactgt
     1501 gcgggtgtcg ataaggttcc gttccctgag cgcatcaagt tcattgagga aaaccacgag
     1561 aacatcatgg cttgcgctaa gtctccactg gagaacactt ggtgggctga gcaagattct
     1621 ccgttctgct tccttgcgtt ctgctttgag tacgctgggg tacagcacca cggcctgagc
     1681 tataactgct cccttccgct ggcgtttgac gggtcttgct ctggcatcca gcacttctcc
     1741 gcgatgctcc gagatgaggt aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt
     1801 caggacatct acgggattgt tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc
     1861 aatgggaccg ataacgaagt agttaccgtg accgatgaga acactggtga aatctctgag
     1921 aaagtcaagc tgggcactaa ggcactggct ggtcaatggc tggcttacgg tgttactcgc
     1981 agtgtgacta agcgttcagt catgacgctg gcttacgggt ccaaagagtt cggcttccgt
     2041 caacaagtgc tggaagatac cattcagcca gctattgatt ccggcaaggg tctgatgttc
     2101 actcagccga atcaggctgc tggatacatg gctaagctga tttgggaatc tgtgagcgtg
     2161 acggtggtag ctgcggttga agcaatgaac tggcttaagt ctgctgctaa gctgctggct
     2221 gctgaggtca aagataagaa gactggagag attcttcgca agcgttgcgc tgtgcattgg
     2281 gtaactcctg atggtttccc tgtgtggcag gaatacaaga agcctattca gacgcgcttg
     2341 aacctgatgt tcctcggtca gttccgctta cagcctacca ttaacaccaa caaagatagc
     2401 gagattgatg cacacaaaca ggagtctggt atcgctccta actttgtaca cagccaagac
     2461 ggtagccacc ttcgtaagac tgtagtgtgg gcacacgaga agtacggaat cgaatctttt
     2521 gcactgattc acgactcctt cggtaccatt ccggctgacg ctgcgaacct gttcaaagca
     2581 gtgcgcgaaa ctatggttga cacatatgag tcttgtgatg tactggctga tttctacgac
     2641 cagttcgctg accagttgca cgagtctcaa ttggacaaaa tgccagcact tccggctaaa
     2701 ggtaacttga acctccgtga catcttagag tcggacttcg cgttcgcgta ataaaggtag
     2761 agacctacta gtagcggccg ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg
     2821 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2881 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2941 aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa
     3001 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3061 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3121 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3181 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3241 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3301 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3361 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3421 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3481 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3541 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3601 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3661 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3721 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3781 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3841 ccagtgctgc aatgataccg cgcgacccac gctcaccggc tccagattta tcagcaataa
     3901 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3961 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4021 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4081 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4141 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4201 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4261 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4321 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     4381 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     4441 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     4501 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     4561 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     4621 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4681 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     4741 cattaaccta taaaaatagg
//
