LOCUS       sequence-236135-        2303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor
            from T7 phage; lethal to host when expressed). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2303)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C98m
FEATURES             Location/Qualifiers
     source          1..2303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..23
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(27..70)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    73..94
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    319..339
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(345..362)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(516..1104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(596..615)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1275..2066,2067..2135))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1898..1917
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2136..2240)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
       61 ggatgatttc tggaattcgc ggccgcttct agagactagt gggtctcaaa tgatgtcaaa
      121 cgtaaataca ggttcactta gtgtggacaa taagaagttt tgggctaccg tagagtcctc
      181 ggagcattcc ttcgaggttc caatctacgc tgagacacta gacgaagctc tggagttagc
      241 cgaatggcaa tacgttccgg ctggctttga ggttactcgt gtgcgtcctt gtgtagcacc
      301 gaagtaaagg tagagaccta ctagtagcgg ccgctgcagg cttcctcgct cactgactcg
      361 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      421 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      481 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
      541 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      601 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      661 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      721 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      781 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      841 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      901 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      961 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1021 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1081 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1141 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1201 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1261 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1321 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1381 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat
     1441 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1501 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1561 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1621 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1681 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1741 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1801 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1861 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1921 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1981 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2041 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2101 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2161 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2221 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2281 attattatca tgacattaac cta
//
