LOCUS       sequence-236137-        9448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector for expression of TetR-EGFP, for visualization of 
            TetO repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Tasan I, Sustackova G, Zhang L, Kim J, Sivaguru M, HamediRad M, Wang
            Y, Genova J, Ma J, Belmont AS, Zhao H
  TITLE     CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live 
            cell imaging of endogenous loci.
  JOURNAL   Nucleic Acids Res. 2018 Jun 15. pii: 5038283. doi: 
            10.1093/nar/gky501.
  PUBMED    29912475
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Jun 15. pii: 5038283. doi: 10.1093/nar/gky501."
COMMENT     Sequence Label: lenti F9-TetR-EGFP-IRES-PuroR
FEATURES             Location/Qualifiers
     source          1..9448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             1091..1714
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1167..1186)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             join(1715..1717,1718..1720,1721..2434)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1715..1717,1718..1720,1721..2431)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1760..1781)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2021..2040)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2368..2389
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2639..3206
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2656..3208
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2800..2817)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3027..3046
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3220..3819
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3220..3239)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3716..3736
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3833..4421
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3886..3906)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4304..4315)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4569..4749
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4796..4814)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4882..4986
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4987..5055,5056..5847)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5205..5224)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6018..6606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6760..6777
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7017..7038
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7053..7058,7059..7076,7077..7083)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7091..7107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7096..7118
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7115..7131
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7115..7131
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7181..7200
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7392..8025
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8072..8197
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8694..8927
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9112..9156
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccagt ttggactagt acgcgtatag atctggatcc
      181 cgccatggta tcaacgccat atttctattt acagtaggga cctcttcgtt gtgtaggtac
      241 cgctgtattc ctagggaaat agtagaggca ccttgaactg tctgcatcag ccatatagcc
      301 cccgctgttc gatttacaaa cacaggcaca gtactgacaa acccatacac ctcctctgaa
      361 atacccatag ttgctagggc tgtctccgaa ctcattacac cctccaaagt cagagctgta
      421 atttcgccat caagggcagc gagggcttct ccagataaaa tagcttctgc cgagagtccc
      481 gtaagggtag acacttcagc taatccctcg atgaggtcta ctagaatagt cagtgcggct
      541 cccattttga aaattcactt acttgatcag cttcagaaga tggcggaggg cctccaacac
      601 agtaattttc ctcccgactc ttaaaataga aaatgtcaag tcagttaagc aggaagtgac
      661 taactgaccg cagctggccg tgcgacatcc tcttttaatt agttgctagg caactgccct
      721 ccagagggca gtgtggtttt gcaagaggaa gcaaaaagcc tctccaccca ggcctggaat
      781 gtttccaccc aatcattact atgacaacag ctgttttttt tagtattaag cagaggccgg
      841 gggcccctgg cctccgctta ctctggagaa aaagaagaga ggcattgtag aggcttccag
      901 aggcaacttg tcaaaacagg actggcgcct tggaggcgct gtggggccac ccaaattgat
      961 ataattaagc cccaaccgcc tcttcccgcc tccagatctc cattcgccat tcaggctgcg
     1021 caactgttgg gaagggcgat cgtctagagt cgacctgcag gggccctcga gatccaaaaa
     1081 ttaggaatta atgatgtcta gattagataa aagtaaagtg attaacagcg cattagagct
     1141 gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt
     1201 agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc
     1261 cattgagatg ttagataggc accatactca cttttgccct ttagaagggg aaagctggca
     1321 agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg
     1381 agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca
     1441 attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc
     1501 tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga
     1561 agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt
     1621 atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg
     1681 attagaaaaa caacttaaat gtgaaagtgg gtccatggtg agcaagggcg aggagctgtt
     1741 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1801 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1861 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     1921 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1981 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     2041 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     2101 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     2161 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     2221 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     2281 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     2341 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     2401 gatcactctc ggcatggacg agctgtacaa gtaagactct ggtcagagat acctggcctg
     2461 gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat
     2521 accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg
     2581 taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg attagagatc
     2641 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     2701 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     2761 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     2821 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     2881 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     2941 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     3001 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     3061 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     3121 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     3181 ttttcctttg aaaaacacga tgataatatg gccacacata tgaccgagta caagcccacg
     3241 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     3301 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     3361 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     3421 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     3481 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     3541 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     3601 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     3661 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     3721 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     3781 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctaaa tcgatagatc ctaatcaacc
     3841 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3901 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3961 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4021 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4081 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4141 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4201 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     4261 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4321 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4381 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa
     4441 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     4501 ggctaattca ctcccaacga agacaagatc acctgcagga caggcgcgcc ctgctttttg
     4561 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4621 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4681 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4741 tctctagcac ccgggcgatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc
     4801 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4861 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4921 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4981 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5041 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5101 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5161 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5221 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5281 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5341 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5401 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5461 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5521 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5581 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5641 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5701 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5761 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5821 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5881 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5941 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6001 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6061 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6121 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6181 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6241 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6301 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6361 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6421 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6481 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6541 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6601 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6661 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6721 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6781 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6841 agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6901 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6961 tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7021 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7081 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg
     7141 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7201 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     7261 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
     7321 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     7381 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
     7441 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     7501 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     7561 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     7621 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     7681 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     7741 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     7801 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     7861 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7921 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7981 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     8041 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     8101 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     8161 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     8221 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     8281 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     8341 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     8401 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     8461 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     8521 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     8581 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     8641 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     8701 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     8761 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     8821 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     8881 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     8941 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9001 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9061 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9121 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     9181 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     9241 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     9301 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     9361 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccga
     9421 attcacaaat ggcagtattc atccacaa
//
