LOCUS       sequence-236141-        2804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for codon-optimized mScarlet-I 
            (fast-folding mScarlet variant, codon-optimized for E. coli; see 
            https://www.fpbase.org/protein/mscarlet-i/)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2804)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C99m
FEATURES             Location/Qualifiers
     source          1..2804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     33..51
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(55..98)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    101..122
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    848..868
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(874..891)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1045..1633)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1125..1144)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1804..2595,2596..2664))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2427..2446
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2665..2769)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca
       61 gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag
      121 agactagtgg gtctcaaatg gtcagtaaag gcgaagcagt tatcaaagag ttcatgcgct
      181 tcaaagttca tatggaaggg tcgatgaacg ggcacgaatt tgaaattgaa ggcgaaggcg
      241 aaggccgccc atatgaaggg acccaaaccg caaagcttaa ggttactaaa ggcggtccat
      301 tacccttttc gtgggacatt ttaagcccac agtttatgta cgggagtcgc gctttcatca
      361 agcaccctgc ggacatccca gattactaca aacagtcttt ccccgagggg ttcaagtggg
      421 agcgcgtgat gaacttcgag gatggcggag ccgtgacggt cacccaagat acctctttgg
      481 aggacggtac gttgatctac aaagtgaaat tgcgtggcac gaattttcca cctgatgggc
      541 ctgtcatgca gaaaaagaca atgggatggg aagcttccac ggagcgcctt tacccagagg
      601 acggtgttct taaaggggat atcaaaatgg cgctgcgtct taaagatgga ggccgctacc
      661 tggcggactt caagactact tacaaggcca aaaaaccagt gcagatgccg ggtgcgtaca
      721 atgtagatcg taaattagat attacaagtc acaatgaaga ttacacggtc gtagagcagt
      781 atgagcgcag tgaggggcgt cactctacgg gcggtatgga cgagttatac aagtaaaggt
      841 agagacctac tagtagcggc cgctgcaggc ttcctcgctc actgactcgc tgcgctcggt
      901 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      961 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1021 taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa
     1081 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1141 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1201 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1261 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1321 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1381 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1441 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1501 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1561 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1621 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1681 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1741 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1801 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     1861 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     1921 ccccagtgct gcaatgatac cgcgcgaccc acgctcaccg gctccagatt tatcagcaat
     1981 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2041 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2101 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2161 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2221 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2281 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2341 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2401 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     2461 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2521 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2581 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2641 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     2701 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2761 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taag
//
