LOCUS       sequence-236142-        6836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains an optimized 48-mer TetO repeat for imaging of desired 
            loci.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Tasan I, Sustackova G, Zhang L, Kim J, Sivaguru M, HamediRad M, Wang
            Y, Genova J, Ma J, Belmont AS, Zhao H
  TITLE     CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live 
            cell imaging of endogenous loci.
  JOURNAL   Nucleic Acids Res. 2018 Jun 15. pii: 5038283. doi: 
            10.1093/nar/gky501.
  PUBMED    29912475
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Jun 15. pii: 5038283. doi: 10.1093/nar/gky501."
COMMENT     Sequence Label: pSP2-48-merTetO-EFS-BLaR
FEATURES             Location/Qualifiers
     source          1..6836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2..20
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    77..95
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    106..124
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    151..169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    180..198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    209..227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    254..272
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    283..301
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    312..330
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    356..374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    385..403
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    414..432
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    459..477
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    488..506
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    517..535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    560..578
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    589..607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    618..636
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    663..681
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    692..710
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    721..739
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    766..784
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    795..813
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    824..842
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    869..887
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    898..916
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    927..945
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    972..990
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1001..1019
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1030..1048
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1065..1098
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1150..1361
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      1369..1378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1375..1773
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    2102..2236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2249..2282
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2329..2520
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        2579..2790
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2797..2806
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2803..3933
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    3988..4109
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4245..4262)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4416..5004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4496..4515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5175..5966,5967..6035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5798..5817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6036..6140)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6208..6226
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    6265..6283
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6294..6312
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6323..6341
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6368..6386
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6397..6415
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6426..6444
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6471..6489
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6500..6518
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6529..6547
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6574..6592
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6603..6621
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6632..6650
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6677..6695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6706..6724
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6735..6753
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6780..6798
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6809..6827
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 atccctatca gtgatagaga gtaaatgtta actaggaaga tgagtttccc tatcagtgat
       61 agagattgta tgaagttccc tatcagtgat agagattatt taggttccct atcagtgata
      121 gagaggtgag ttggactagg taatagatgg tccctatcag tgatagagag gggtattggt
      181 ccctatcagt gatagagagt atagttattc cctatcagtg atagagaaga tagtgatact
      241 aggaggagtt gggtccctat cagtgataga gattagagat ggtccctatc agtgatagag
      301 agtaggaaat ttccctatca gtgatagaga taaagggtga ctaggaagta ttgaatccct
      361 atcagtgata gagaatatat tgtgtcccta tcagtgatag agagtagggt ggttccctat
      421 cagtgataga gaaggtgggg atactaggtg ggtgatattc cctatcagtg atagagatta
      481 tagagtgtcc ctatcagtga tagagatagg gttgattccc tatcagtgat agagaaggtt
      541 tggaaactag gtagaggtat ccctatcagt gatagagagg tgttagggtc cctatcagtg
      601 atagagagga taaagtgtcc ctatcagtga tagagatggg ttaaagacta ggtagtggaa
      661 agtccctatc agtgatagag agatttttat ttccctatca gtgatagaga gaggggataa
      721 tccctatcag tgatagagat tgtagagtta ctagggaagt ggttgtccct atcagtgata
      781 gagagtagag ggagtcccta tcagtgatag agaaaaggtg gtttccctat cagtgataga
      841 gaataagtta gtactagggt gtaatttatc cctatcagtg atagagagta tagagggtcc
      901 ctatcagtga tagagagtaa tatggttccc tatcagtgat agagatggag gtgggactag
      961 gagtttagat ttccctatca gtgatagaga ggtaattttg tccctatcag tgatagagat
     1021 tagtatggtt ccctatcagt gatagagagg gttgtgatac tagtataact tcgtataatg
     1081 tatgctatac gaagttatca tatggctagg tcttgaaagg agtgggaatt ggctccggtg
     1141 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     1201 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     1261 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     1321 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaagcttgc caccatggcc
     1381 aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc
     1441 atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc
     1501 ttcactggtg tcaatgtata tcattttact gggggacctt gcgcagaact cgtggtgctg
     1561 ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag
     1621 aacaggggca tcttgagccc ctgcggacgg tgccgacagg ttcttctcga tctgcatcct
     1681 gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa
     1741 ttgctgccct ctggttatgt gtgggagggc taacccttat acgcaaggga gtagatgccg
     1801 accgaacaag agctgatttc gagaacgcct cagccagcaa ctcgcgcgag cctagcaagg
     1861 caaatgcgag agaacggcct tacgcttggt ggcacagttc tcgtccacag ttcgctaagc
     1921 tcgctcggct gggtcgcggg agggccggtc gcagtgattc aggcccttct ggattgtgtt
     1981 ggtccccagg gcacgattgt catgcccacg cactcgggtg atctgactga tcccgcagat
     2041 tggagatcgc cgcccgtgcc tgccgattgg gtgcagatca gcctcgaggc cagctagctt
     2101 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     2161 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     2221 ttatcatgtc tggatcacat gtttcgaaat aacttcgtat aatgtatgct atacgaagtt
     2281 atctcgagag cgctgctagc ttaaggtacc atcgatagat ctgggcccgt gaccaaacag
     2341 gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag
     2401 aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac
     2461 gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     2521 cttaagcctg agggctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2581 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc
     2641 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     2701 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     2761 tttcgcaacg ggtttgccgc cagaacacag gggcgcgcca ccatggcttc gtacccctgc
     2821 catcaacacg cgtctgcgtt cgaccaggct gcgcgttctc gcggccatag caaccgacgt
     2881 acggcgttgc gccctcgccg gcagcaagaa gccacggaag tccgcctgga gcagaaaatg
     2941 cccacgctac tgcgggttta tatagacggt cctcacggga tggggaaaac caccaccacg
     3001 caactgctgg tggccctggg ttcgcgcgac gatatcgtct acgtacccga gccgatgact
     3061 tactggcagg tgctgggggc ttccgagaca atcgcgaaca tctacaccac acaacaccgc
     3121 ctcgaccagg gtgagatatc ggccggggac gcggcggtgg taatgacaag cgcccagata
     3181 acaatgggca tgccttatgc cgtgaccgac gccgttctgg ctcctcatat cgggggggag
     3241 gctgggagct cacatgcccc gcccccggcc ctcaccctca tcttcgaccg ccatcccatc
     3301 gccgccctcc tgtgctaccc ggccgcgcga taccttatgg gcagcatgac cccccaggcc
     3361 gtgctggcgt tcgtggccct catcccgccg accttgcccg gcacaaacat cgtgttgggg
     3421 gcccttccgg aggacagaca catcgaccgc ctggccaaac gccagcgccc cggcgagcgg
     3481 cttgacctgg ctatgctggc cgcgattcgc cgcgtttacg ggctgcttgc caatacggtg
     3541 cggtatctgc agggcggcgg gtcgtggcgg gaggattggg gacagctttc ggggacggcc
     3601 gtgccgcccc agggtgccga gccccagagc aacgcgggcc cacgacccca tatcggggac
     3661 acgttattta ccctgtttcg ggcccccgag ttgctggccc ccaacggcga cctgtacaac
     3721 gtgtttgcct gggccttgga cgtcttggcc aaacgcctcc gtcccatgca cgtctttatc
     3781 ctggattacg accaatcgcc cgccggctgc cgggacgccc tgctgcaact tacctccggg
     3841 atggtccaga cccacgtcac cacccccggc tccataccga cgatctgcga cctggcgcgc
     3901 acgtttgccc gggagatggg ggaggctaac tgacgatgat aacgcgactc tagagtcggg
     3961 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     4021 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     4081 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     4141 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     4201 aaatcgataa ggatccgttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     4261 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4321 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4381 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4441 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4501 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4561 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4621 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4681 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4741 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4801 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     4861 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4921 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4981 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5041 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5101 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5161 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     5221 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     5281 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     5341 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     5401 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     5461 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     5521 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5581 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5641 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5701 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5761 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     5821 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5881 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     5941 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6001 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6061 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6121 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     6181 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     6241 gaattcgccc taggaatggg ttagtcccta tcagtgatag agagaagggt gtttccctat
     6301 cagtgataga gatggtggtg attccctatc agtgatagag aattgaaatt tactaggaga
     6361 atagaagtcc ctatcagtga tagagaatat tggtgttccc tatcagtgat agagagggaa
     6421 tagtttccct atcagtgata gagatagttt ggggactagg gaagagtttg tccctatcag
     6481 tgatagagag tgtgtatagt ccctatcagt gatagagaag aagtaatgtc cctatcagtg
     6541 atagagaagt taatgtaact aggaaaaata taatccctat cagtgataga gaaattttga
     6601 gatccctatc agtgatagag attgggagtg gtccctatca gtgatagaga tggaagaagg
     6661 actagggaat gtatgatccc tatcagtgat agagaagagt tagtgtccct atcagtgata
     6721 gagagtagtt ggattcccta tcagtgatag agataggggt gatactaggt gggggattgt
     6781 ccctatcagt gatagagagg aaaaaggttc cctatcagtg atagagagtt ggtgga
//
