LOCUS       sequence-236143-        2903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part sfYFP-ptd3 (superfolder YFP with 
            pdt3 degradation tag for mfLon). Please see Supplemental Documents 
            for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2903)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C100m
FEATURES             Location/Qualifiers
     source          1..2903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     11..29
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(33..76)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    79..100
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             116..829
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    925..945
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(951..968)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1122..1710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1202..1221)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1881..2672,2673..2741))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2504..2523
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2742..2846)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
       61 cgctaaggat gatttctgga attcgcggcc gcttctagag actagtgggt ctcaaatgcg
      121 taaaggcgaa gagctgttca ctggtgtcgt ccctattctg gtggaactgg atggtgatgt
      181 caacggtcat aagttttccg tgcgtggcga gggtgaaggt gacgcaacta atggtaaact
      241 gacgctgaag ttcatctgta ctactggtaa actgccggta ccttggccga ctctggtaac
      301 gacgctgact tatggtgttc agtgctttgc tcgttatccg gaccatatga agcagcatga
      361 cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt cctttaagga
      421 tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg
      481 cattgagctg aaaggcattg actttaaaga agacggcaat atcctgggcc ataagctgga
      541 atacaatttt aacagccaca atgtttacat caccgccgat aaacaaaaaa atggcattaa
      601 agcgaatttt aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg ctgatcacta
      661 ccagcaaaac actccaatcg gtgatggtcc tgttctgctg ccagacaatc actatctgag
      721 ctaccaaagc gttctgtcta aagatccgaa cgagaaacgc gatcatatgg ttctgctgga
      781 gttcgtaacc gcagcgggca tcacgcatgg tatggatgaa ctgtacaaag cggcgaacaa
      841 aaacgaagaa aacaccaacg aagtgccgac ctttatgctg aacgcgggcc aggcgaacag
      901 aagacgagtt taaaggtaga gacctactag tagcggccgc tgcaggcttc ctcgctcact
      961 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1021 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1081 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc
     1141 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1201 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1261 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1321 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1381 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1441 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1501 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1561 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1621 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1681 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1741 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1801 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1861 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1921 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1981 gagggcttac catctggccc cagtgctgca atgataccgc gcgacccacg ctcaccggct
     2041 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2101 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     2161 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     2221 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     2281 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     2341 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     2401 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     2461 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     2521 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     2581 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     2641 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     2701 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     2761 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2821 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     2881 gaaaccatta ttatcatgac att
//
