LOCUS       sequence-236148-        8484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains an optimized 96-mer TetO repeat for imaging of desired 
            loci.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8484)
  AUTHORS   Tasan I, Sustackova G, Zhang L, Kim J, Sivaguru M, HamediRad M, Wang
            Y, Genova J, Ma J, Belmont AS, Zhao H
  TITLE     CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live 
            cell imaging of endogenous loci.
  JOURNAL   Nucleic Acids Res. 2018 Jun 15. pii: 5038283. doi: 
            10.1093/nar/gky501.
  PUBMED    29912475
REFERENCE   2  (bases 1 to 8484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Jun 15. pii: 5038283. doi: 10.1093/nar/gky501."
COMMENT     Sequence Label: pSP2-96-merTetO-EFS-BLaR
FEATURES             Location/Qualifiers
     source          1..8484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    46..64
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    75..93
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    104..122
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    147..165
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    176..194
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    205..223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    250..268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    279..297
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    308..326
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    353..371
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    382..400
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    411..429
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    456..474
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    485..503
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    514..532
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    559..577
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    588..606
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    617..635
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    662..680
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    691..709
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    720..738
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    765..783
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    794..812
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    823..841
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    868..886
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    897..915
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    926..944
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    971..989
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1000..1018
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1029..1047
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1074..1092
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1103..1121
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1132..1150
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1177..1195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1206..1224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1235..1253
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1280..1298
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1309..1327
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1338..1356
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1383..1401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1412..1430
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1441..1459
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1486..1504
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1515..1533
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1544..1562
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1589..1607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1618..1636
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1647..1665
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1692..1710
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1721..1739
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1750..1768
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1795..1813
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1824..1842
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1853..1871
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1898..1916
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1927..1945
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1956..1974
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2001..2019
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2030..2048
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2059..2077
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2104..2122
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2133..2151
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2162..2180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2207..2225
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2236..2254
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2265..2283
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2300..2333
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2385..2596
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2604..2613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2610..3008
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3337..3471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3484..3517
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3564..3755
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        3814..4025
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      4032..4041
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4038..5168
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    5223..5344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5480..5497)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5651..6239)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5731..5750)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6410..7201,7202..7270))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7033..7052
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7271..7375)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7443..7461
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    7500..7518
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7529..7547
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7558..7576
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7603..7621
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7632..7650
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7661..7679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7706..7724
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7735..7753
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7764..7782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7809..7827
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7838..7856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7867..7885
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7912..7930
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7941..7959
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7970..7988
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8015..8033
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8044..8062
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8073..8091
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8118..8136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8148..8166
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8177..8195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8222..8240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8251..8269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8280..8298
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8325..8343
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8354..8372
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8383..8401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8427..8445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8456..8474
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tccctatcag tgatagagaa ggtggggata ctaggtgggt gatattccct atcagtgata
       61 gagattatag agtgtcccta tcagtgatag agatagggtt gattccctat cagtgataga
      121 gaaggtttgg aaactaggta gaggtatccc tatcagtgat agagaggtgt tagggtccct
      181 atcagtgata gagaggataa agtgtcccta tcagtgatag agatgggtta aagactaggt
      241 agtggaaagt ccctatcagt gatagagaga tttttatttc cctatcagtg atagagagag
      301 gggataatcc ctatcagtga tagagattgt agagttacta gggaagtggt tgtccctatc
      361 agtgatagag agtagaggga gtccctatca gtgatagaga aaaggtggtt tccctatcag
      421 tgatagagaa taagttagta ctagggtgta atttatccct atcagtgata gagagtatag
      481 agggtcccta tcagtgatag agagtaatat ggttccctat cagtgataga gatggaggtg
      541 ggactaggag tttagatttc cctatcagtg atagagaggt aattttgtcc ctatcagtga
      601 tagagattag tatggttccc tatcagtgat agagagggtt gtgatactag ggaggtggag
      661 atccctatca gtgatagaga gtaaggtagt tccctatcag tgatagagag gtgaaggtgt
      721 ccctatcagt gatagagata tataggaaac taggggagta gttgtcccta tcagtgatag
      781 agagtatgga ggttccctat cagtgataga gatgggaaaa agtccctatc agtgatagag
      841 agatagtggg gactaggaat agatttatcc ctatcagtga tagagatttt aatttttccc
      901 tatcagtgat agagagaggt tagattccct atcagtgata gagaggaggt ggagactagg
      961 gttaagataa tccctatcag tgatagagat gtatgggtat ccctatcagt gatagagatg
     1021 gggggtggtc cctatcagtg atagagatga aagtgagact aggaagttat gagtccctat
     1081 cagtgataga gatgaaaatt attccctatc agtgatagag atttgagagt atccctatca
     1141 gtgatagaga ataattgaat actagggaat tgtagttccc tatcagtgat agagattatg
     1201 gggggtccct atcagtgata gagatgtggt gtggtcccta tcagtgatag agaaaagatt
     1261 gtaactaggt tatgttgtat ccctatcagt gatagagaga gaagataatc cctatcagtg
     1321 atagagaggg agatgaatcc ctatcagtga tagagagtaa aagtttacta gggattttag
     1381 attccctatc agtgatagag aagtggggat gtccctatca gtgatagaga tgtagtatat
     1441 tccctatcag tgatagagag ttttaggaaa ctaggaagtg tatattccct atcagtgata
     1501 gagaagagat gttgtcccta tcagtgatag agaaagggtg ggttccctat cagtgataga
     1561 gagtaattta taactaggtg atgttggttc cctatcagtg atagagaaag gtagatatcc
     1621 ctatcagtga tagagatggg agagtttccc tatcagtgat agagaagatg ttaagactag
     1681 gttagtgggt gtccctatca gtgatagaga taaaggtgtg tccctatcag tgatagagag
     1741 gatatatgtt ccctatcagt gatagagaat ggaattggac taggtgttgt ttggtcccta
     1801 tcagtgatag agagtggttg atttccctat cagtgataga gagtattgtt agtccctatc
     1861 agtgatagag agttaatttg gactaggtag gaataggtcc ctatcagtga tagagaatgg
     1921 gttttatccc tatcagtgat agagataggg taaattccct atcagtgata gagattaaga
     1981 taatactagg tagtaggtgt tccctatcag tgatagagat tgtatttggt ccctatcagt
     2041 gatagagagg gggtgtgttc cctatcagtg atagagaatt gatagatact agggtatata
     2101 tgttccctat cagtgataga gaaatttaaa agtccctatc agtgatagag aattggggtt
     2161 gtccctatca gtgatagaga tgttttatat actagggaga tgaatttccc tatcagtgat
     2221 agagatgggt ttgagtccct atcagtgata gagaggaaaa ggattcccta tcagtgatag
     2281 agataggttg atgactagta taacttcgta taatgtatgc tatacgaagt tatcatatgg
     2341 ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc
     2401 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgatccggtg cctagagaag
     2461 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     2521 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     2581 tgccgccaga acacaggaag cttgccacca tggccaagcc tttgtctcaa gaagaatcca
     2641 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     2701 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     2761 ttactggggg accttgcgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     2821 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     2881 gacggtgccg acaggttctt ctcgatctgc atcctgggat caaagccata gtgaaggaca
     2941 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     3001 agggctaacc cttatacgca agggagtaga tgccgaccga acaagagctg atttcgagaa
     3061 cgcctcagcc agcaactcgc gcgagcctag caaggcaaat gcgagagaac ggccttacgc
     3121 ttggtggcac agttctcgtc cacagttcgc taagctcgct cggctgggtc gcgggagggc
     3181 cggtcgcagt gattcaggcc cttctggatt gtgttggtcc ccagggcacg attgtcatgc
     3241 ccacgcactc gggtgatctg actgatcccg cagattggag atcgccgccc gtgcctgccg
     3301 attgggtgca gatcagcctc gaggccagct agcttgaact tgtttattgc agcttataat
     3361 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3421 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat cacatgtttc
     3481 gaaataactt cgtataatgt atgctatacg aagttatctc gagagcgctg ctagcttaag
     3541 gtaccatcga tagatctggg cccgtgacca aacaggaaaa aaccgccctt aacatggccc
     3601 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg
     3661 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc
     3721 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacttaa gcctgagggc taggtcttga
     3781 aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     3841 ccgagaagtt ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg
     3901 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     3961 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     4021 cacaggggcg cgccaccatg gcttcgtacc cctgccatca acacgcgtct gcgttcgacc
     4081 aggctgcgcg ttctcgcggc catagcaacc gacgtacggc gttgcgccct cgccggcagc
     4141 aagaagccac ggaagtccgc ctggagcaga aaatgcccac gctactgcgg gtttatatag
     4201 acggtcctca cgggatgggg aaaaccacca ccacgcaact gctggtggcc ctgggttcgc
     4261 gcgacgatat cgtctacgta cccgagccga tgacttactg gcaggtgctg ggggcttccg
     4321 agacaatcgc gaacatctac accacacaac accgcctcga ccagggtgag atatcggccg
     4381 gggacgcggc ggtggtaatg acaagcgccc agataacaat gggcatgcct tatgccgtga
     4441 ccgacgccgt tctggctcct catatcgggg gggaggctgg gagctcacat gccccgcccc
     4501 cggccctcac cctcatcttc gaccgccatc ccatcgccgc cctcctgtgc tacccggccg
     4561 cgcgatacct tatgggcagc atgacccccc aggccgtgct ggcgttcgtg gccctcatcc
     4621 cgccgacctt gcccggcaca aacatcgtgt tgggggccct tccggaggac agacacatcg
     4681 accgcctggc caaacgccag cgccccggcg agcggcttga cctggctatg ctggccgcga
     4741 ttcgccgcgt ttacgggctg cttgccaata cggtgcggta tctgcagggc ggcgggtcgt
     4801 ggcgggagga ttggggacag ctttcgggga cggccgtgcc gccccagggt gccgagcccc
     4861 agagcaacgc gggcccacga ccccatatcg gggacacgtt atttaccctg tttcgggccc
     4921 ccgagttgct ggcccccaac ggcgacctgt acaacgtgtt tgcctgggcc ttggacgtct
     4981 tggccaaacg cctccgtccc atgcacgtct ttatcctgga ttacgaccaa tcgcccgccg
     5041 gctgccggga cgccctgctg caacttacct ccgggatggt ccagacccac gtcaccaccc
     5101 ccggctccat accgacgatc tgcgacctgg cgcgcacgtt tgcccgggag atgggggagg
     5161 ctaactgacg atgataacgc gactctagag tcggggcggc cggccgcttc gagcagacat
     5221 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     5281 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     5341 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     5401 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtttgcgta
     5461 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5521 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5581 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5641 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5701 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5761 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5821 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     5881 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5941 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6001 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6061 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     6121 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6181 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6241 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6301 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6361 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6421 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6481 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     6541 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     6601 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6661 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6721 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6781 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     6841 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     6901 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     6961 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7021 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7081 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7141 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7201 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7261 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7321 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7381 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     7441 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt cgccctagga atgggttagt
     7501 ccctatcagt gatagagaga agggtgtttc cctatcagtg atagagatgg tggtgattcc
     7561 ctatcagtga tagagaattg aaatttacta ggagaataga agtccctatc agtgatagag
     7621 aatattggtg ttccctatca gtgatagaga gggaatagtt tccctatcag tgatagagat
     7681 agtttgggga ctagggaaga gtttgtccct atcagtgata gagagtgtgt atagtcccta
     7741 tcagtgatag agaagaagta atgtccctat cagtgataga gaagttaatg taactaggaa
     7801 aaatataatc cctatcagtg atagagaaat tttgagatcc ctatcagtga tagagattgg
     7861 gagtggtccc tatcagtgat agagatggaa gaaggactag ggaatgtatg atccctatca
     7921 gtgatagaga agagttagtg tccctatcag tgatagagag tagttggatt ccctatcagt
     7981 gatagagata ggggtgatac taggtggggg attgtcccta tcagtgatag agaggaaaaa
     8041 ggttccctat cagtgataga gagttggtgg aatccctatc agtgatagag agtaaatgtt
     8101 aactaggaag atgagtttcc ctatcagtga tagagattgt atgaagttcc ctatcagtga
     8161 tagagattat ttaggttccc tatcagtgat agagaggtga gttggactag gtaatagatg
     8221 gtccctatca gtgatagaga ggggtattgg tccctatcag tgatagagag tatagttatt
     8281 ccctatcagt gatagagaag atagtgatac taggaggagt tgggtcccta tcagtgatag
     8341 agattagaga tggtccctat cagtgataga gagtaggaaa tttccctatc agtgatagag
     8401 ataaagggtg actaggaagt attgaatccc tatcagtgat agagaatata ttgtgtccct
     8461 atcagtgata gagagtaggg tggt
//
