LOCUS       sequence-236150-        6058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An adeno-associated viral vector expressing HA-tagged inhibitory 
            DREADD (hM4D(Gi)) from a synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Galvan A, Raper J, Hu X, Pare JF, Bonaventura J, Richie CT, 
            Michaelides M, Mueller SAL, Roseboom PH, Oler JA, Kalin NH, Hall RA,
            Smith Y
  TITLE     Ultrastructural localization of DREADDs in monkeys.
  JOURNAL   Eur J Neurosci. 2019 May 7. doi: 10.1111/ejn.14429.
  PUBMED    31063250
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Neurosci. 2019 May 7. doi: 10.1111/ejn.14429."
COMMENT     Sequence Label: pOTTC1484 - pAAV SYN1 HA-hM4D(Gi)
FEATURES             Location/Qualifiers
     source          1..6058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             678..704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2185..2773
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2238..2258)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2656..2667)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2805..3281
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3004..3023)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3321..3461
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3321..3450
                     /label=AAV2 ITR
     rep_origin      3536..3991
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3623..3642)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3833..3854
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4008..4027)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4127..4149
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4187..4205)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4273..4377
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4378..4446,4447..5238)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4596..4615)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5409..5997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5898..5917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccggatc cgaagttatc gctagctcta
      661 gagtcgacgc caccatgtac ccatacgatg ttccagatta cgctatggcc aacttcacac
      721 cagtcaatgg cagctcgggc aatcagtccg tgcgcctggt cacgtcatca tcccacaatc
      781 gctatgagac ggtggaaatg gtcttcattg ccacagtgac aggctccctg agcctggtga
      841 ctgtcgtggg caacatcctg gtgatgctgt ccatcaaggt caacaggcag ctgcagacag
      901 tcaacaacta cttcctcttc agcctggcgt gtgctgatct catcataggc gccttctcca
      961 tgaacctcta caccgtgtac atcatcaagg gctactggcc cctgggcgcc gtggtctgcg
     1021 acctgtggct ggccctggac tgcgtggtga gcaacgcctc cgtcatgaac cttctcatca
     1081 tcagctttga ccgctacttc tgcgtcacca agcctctcac ctaccctgcc cggcgcacca
     1141 ccaagatggc aggcctcatg attgctgctg cctgggtact gtccttcgtg ctctgggcgc
     1201 ctgccatctt gttctggcag tttgtggtgg gtaagcggac ggtgcccgac aaccagtgct
     1261 tcatccagtt cctgtccaac ccagcagtga cctttggcac agccattgct ggcttctacc
     1321 tgcctgtggt catcatgacg gtgctgtaca tccacatctc cctggccagt cgcagccgag
     1381 tccacaagca ccggcccgag ggcccgaagg agaagaaagc caagacgctg gccttcctca
     1441 agagcccact aatgaagcag agcgtcaaga agcccccgcc cggggaggcc gcccgggagg
     1501 agctgcgcaa tggcaagctg gaggaggccc ccccgccagc gctgccaccg ccaccgcgcc
     1561 ccgtggctga taaggacact tccaatgagt ccagctcagg cagtgccacc cagaacacca
     1621 aggaacgccc agccacagag ctgtccacca cagaggccac cacgcccgcc atgcccgccc
     1681 ctcccctgca gccgcgggcc ctcaacccag cctccagatg gtccaagatc cagattgtga
     1741 cgaagcagac aggcaatgag tgtgtgacag ccattgagat tgtgcctgcc acgccggctg
     1801 gcatgcgccc tgcggccaac gtggcccgca agttcgccag catcgctcgc aaccaggtgc
     1861 gcaagaagcg gcagatggcg gcccgggagc gcaaagtgac acgaacgatc tttgccattc
     1921 tgcttgcctt catcctcacc tggacgccct acaacgtcat ggtcctggtg aacaccttct
     1981 gccagagctg catccctgac acggtgtggt ccattggcta ctggctctgc tacgtcaaca
     2041 gcaccatcaa ccctgcctgc tatgctctgt gcaacgccac ctttaaaaag accttccggc
     2101 acctgctgct gtgccagtat cggaacatcg gcactgccag gtagggcgcg ccataacttc
     2161 gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     2221 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2281 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     2341 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     2401 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     2461 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     2521 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     2581 tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc
     2641 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     2701 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     2761 gccgcctccc cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg catccctgtg
     2821 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg
     2881 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg
     2941 ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg
     3001 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
     3061 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
     3121 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg
     3181 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc
     3241 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg taggtaacca
     3301 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     3361 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     3421 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     3481 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     3541 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3601 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3661 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3721 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     3781 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3841 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
     3901 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3961 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     4021 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4081 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     4141 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     4201 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     4261 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4321 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4381 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4441 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4501 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4561 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4621 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4681 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4741 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4801 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4861 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4921 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4981 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5041 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5101 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5161 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5221 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5281 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5341 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5401 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5461 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5521 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5581 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5641 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5701 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5761 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5821 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5881 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5941 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6001 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
