LOCUS       sequence-236161-        7423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent reporter of cytosolic hydrogen peroxide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Langford TF, Huang BK, Lim JB, Moon SJ, Sikes HD
  TITLE     Monitoring the action of redox-directed cancer therapeutics using a 
            human peroxiredoxin-2-based probe.
  JOURNAL   Nat Commun. 2018 Aug 7;9(1):3145. doi: 10.1038/s41467-018-05557-y.
  PUBMED    30087344
REFERENCE   2  (bases 1 to 7423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-08-7"; volume: "9(1):3145. doi"; pages: " 
            10.1038/s41467-018-05557-y"
COMMENT     Sequence Label: pcDNA3.1-Clover-Prx2-mRuby2
FEATURES             Location/Qualifiers
     source          1..7423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     534..555
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1186..1896
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     primer_bind     complement(1937..1954)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1943..2167
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2213..2641
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2300..2319)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2510..2531
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2650..2670)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2655..2984
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2835..2970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2897..2916
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3051..3845
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3105..3124)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3715..3734
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4019..4140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4056..4075)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4110..4129
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4189..4205)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4189..4205)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4202..4224)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4213..4229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4237..4243,4244..4261,4262..4267))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4282..4303
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4420..4437)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4591..5179)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4671..4690)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5350..6141,6142..6210))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5973..5992
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6211..6315)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6390..6409)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6581..6960
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6961..7164
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7115..7135
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7209..7228
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7209..7227
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7349..7370)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
       61 ctcgtgacca ccttcggcta cggcgtggcc tgcttcagcc gctaccccga ccacatgaag
      121 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatctct
      181 ttcaaggacg acggtaccta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      241 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      301 aagctggagt acaacttcaa cagccacaac gtctatatca cggccgacaa gcagaagaac
      361 ggcatcaagg ctaacttcaa gatccgccac aacgttgagg acggcagcgt gcagctcgcc
      421 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      481 tacctgagcc atcagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      541 ctgctggagt tcgtgaccgc cagcggtggt ggtagcatgg cctccggtaa cgcgcgcatc
      601 ggaaagccag cccctgactt caaggccaca gcggtggttg atggcgcctt caaagaggtg
      661 aagctgtcgg actacaaagg gaagtacgtg gtcctctttt tctaccctct ggacttcacc
      721 tttgtgtgcc ccaccgagat catcgcgttc agcaaccgtg cagaggactt ccgcaagctg
      781 ggctgtgaag tgctgggcgt ctcggtggac tctcagttca cccacctggc ttggatcaac
      841 accccccgga aagagggagg cttgggcccc ctgaacatcc ccctgcttgc tgacgtgacc
      901 agacgcttgt ctgaggatta cggcgtgctg aaaacagatg agggcattgc ctacaggggc
      961 ctctttatca tcgatggcaa gggtgtcctt cgccagatca ctgttaatga tttgcctgtg
     1021 ggacgctccg tggatgaggc tctgcggctg gtccaggcct tccagtacac agacgagcat
     1081 ggggaagttt gtcccgctgg ctggaagcct ggcagtgaca cgattaagcc caacgtggat
     1141 gacagcaagg aatatttctc caaacacaat agcggtggtg gtagcatggt gtctaagggc
     1201 gaagagctga tcaaggaaaa tatgcgtatg aaggtggtca tggaaggttc ggtcaacggc
     1261 caccaattca aatgcacagg tgaaggagaa ggcaatccgt acatgggaac tcaaaccatg
     1321 aggatcaaag tcatcgaggg aggacccctg ccatttgcct ttgacattct tgccacgtcg
     1381 ttcatgtatg gcagccgtac ttttatcaag tacccgaaag gcattcctga tttctttaaa
     1441 cagtcctttc ctgagggttt tacttgggaa agagttacga gatacgaaga tggtggagtc
     1501 gtcaccgtca tgcaggacac cagccttgag gatggctgtc tcgtttacca cgtccaagtc
     1561 agaggggtaa actttccctc caatggtccc gtgatgcaga agaagaccaa gggttgggag
     1621 cctaatacag agatgatgta tccagcagat ggtggtctga ggggatacac tcatatggca
     1681 ctgaaagttg atggtggtgg ccatctgtct tgctctttcg taacaactta caggtcaaaa
     1741 aagaccgtcg ggaacatcaa gatgcccggt atccatgccg ttgatcaccg cctggaaagg
     1801 ttagaggaaa gtgacaatga aatgttcgta gtacaacgcg aacacgcagt tgccaagttc
     1861 gccgggcttg gtggtgggat ggacgagctg tacaagtaac tcgagtctag agggcccgtt
     1921 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     1981 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     2041 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     2101 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     2161 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     2221 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2281 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2341 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     2401 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     2461 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     2521 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     2581 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     2641 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     2701 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     2761 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     2821 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     2881 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     2941 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     3001 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     3061 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     3121 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     3181 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     3241 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     3301 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     3361 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     3421 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     3481 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     3541 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     3601 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     3661 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     3721 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     3781 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     3841 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     3901 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     3961 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     4021 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4081 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4141 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     4201 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     4261 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4321 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     4381 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4441 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4501 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4561 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4621 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4681 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4741 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4801 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4861 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4921 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4981 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     5041 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5101 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5161 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5221 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5281 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     5341 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     5401 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     5461 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     5521 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     5581 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     5641 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     5701 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     5761 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     5821 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     5881 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5941 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     6001 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     6061 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     6121 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6181 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6241 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     6301 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     6361 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     6421 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     6481 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     6541 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     6601 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     6661 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     6721 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     6781 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     6841 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     6901 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     6961 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     7021 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     7081 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     7141 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
     7201 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt aaacttaagc
     7261 ttggtaccga gctcggatcc actagtccag tgtggtggaa ttcatggtga gcaagggcga
     7321 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     7381 caagttcagc gtccgcggcg agggcgaggg cgatgccacc aac
//
