LOCUS       sequence-236162-        2846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding 
            mScarlet variant, codon-optimized for E. col, with LAA degradation 
            tag; see https://www.fpbase.org/protein/mscarlet-i/)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2846)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C103m
FEATURES             Location/Qualifiers
     source          1..2846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     25..43
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(47..90)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    93..114
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    882..902
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(908..925)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1079..1667)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1159..1178)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1838..2629,2630..2698))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2461..2480
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2699..2803)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa
       61 aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agagactagt
      121 gggtctcaaa tgatggtcag taaaggcgaa gcagttatca aagagttcat gcgcttcaaa
      181 gttcatatgg aagggtcgat gaacgggcac gaatttgaaa ttgaaggcga aggcgaaggc
      241 cgcccatatg aagggaccca aaccgcaaag cttaaggtta ctaaaggcgg tccattaccc
      301 ttttcgtggg acattttaag cccacagttt atgtacggga gtcgcgcttt catcaagcac
      361 cctgcggaca tcccagatta ctacaaacag tctttccccg aggggttcaa gtgggagcgc
      421 gtgatgaact tcgaggatgg cggagccgtg acggtcaccc aagatacctc tttggaggac
      481 ggtacgttga tctacaaagt gaaattgcgt ggcacgaatt ttccacctga tgggcctgtc
      541 atgcagaaaa agacaatggg atgggaagct tccacggagc gcctttaccc agaggacggt
      601 gttcttaaag gggatatcaa aatggcgctg cgtcttaaag atggaggccg ctacctggcg
      661 gacttcaaga ctacttacaa ggccaaaaaa ccagtgcaga tgccgggtgc gtacaatgta
      721 gatcgtaaat tagatattac aagtcacaat gaagattaca cggtcgtaga gcagtatgag
      781 cgcagtgagg ggcgtcactc tacgggcggt atggacgagt tatacaagac tagtgcagcg
      841 aacgacgaaa attacgccct tgcagcgtga aggtagagac ctactagtag cggccgctgc
      901 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      961 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1021 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1081 tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1141 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1201 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1261 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1321 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1381 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1441 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1501 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1561 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1621 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1681 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1741 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1801 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     1861 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1921 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgcg
     1981 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2041 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2101 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2161 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2221 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2281 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2341 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2401 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2461 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2521 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2581 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2641 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2701 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     2761 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     2821 tgccacctga cgtctaagaa accatt
//
