LOCUS       sequence-236163-        2861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP 
            with AAV degradation tag for ssrA). Please see Supplemental 
            Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2861)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C105m
FEATURES             Location/Qualifiers
     source          1..2861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..37
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(41..84)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    87..108
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             124..837
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    891..911
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(917..934)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1088..1676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1168..1187)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1847..2638,2639..2707))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2470..2489
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2708..2812)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc
       61 ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagac tagtgggtct
      121 caaatgcgta aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt ggaactggat
      181 ggtgatgtca acggtcataa gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat
      241 ggtaaactga cgctgaagtt catctgtact actggtaaac tgccggtacc ttggccgact
      301 ctggtaacga cgctgactta tggtgttcag tgctttgctc gttatccgga ccatatgaag
      361 cagcatgact tcttcaagtc cgccatgccg gaaggctatg tgcaggaacg cacgatttcc
      421 tttaaggatg acggcacgta caaaacgcgt gcggaagtga aatttgaagg cgataccctg
      481 gtaaaccgca ttgagctgaa aggcattgac tttaaagaag acggcaatat cctgggccat
      541 aagctggaat acaattttaa cagccacaat gtttacatca ccgccgataa acaaaaaaat
      601 ggcattaaag cgaattttaa aattcgccac aacgtggagg atggcagcgt gcagctggct
      661 gatcactacc agcaaaacac tccaatcggt gatggtcctg ttctgctgcc agacaatcac
      721 tatctgagct accaaagcgt tctgtctaaa gatccgaacg agaaacgcga tcatatggtt
      781 ctgctggagt tcgtaaccgc agcgggcatc acgcatggta tggatgaact gtacaaaact
      841 agtgcagcga acgacgaaaa ttacgccgct gctgtttgaa ggtagagacc tactagtagc
      901 ggccgctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      961 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     1021 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     1081 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     1141 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1201 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1261 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1321 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1381 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1441 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1501 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1561 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1621 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1681 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1741 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1801 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1861 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1921 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1981 taccgcgcga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2041 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2101 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     2161 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     2221 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     2281 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     2341 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2401 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2461 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2521 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2581 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2641 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     2701 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2761 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     2821 cccgaaaagt gccacctgac gtctaagaaa ccattattat c
//
