LOCUS       sequence-236164-       10057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent reporter of cytosolic hydrogen peroxide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10057)
  AUTHORS   Langford TF, Huang BK, Lim JB, Moon SJ, Sikes HD
  TITLE     Monitoring the action of redox-directed cancer therapeutics using a 
            human peroxiredoxin-2-based probe.
  JOURNAL   Nat Commun. 2018 Aug 7;9(1):3145. doi: 10.1038/s41467-018-05557-y.
  PUBMED    30087344
REFERENCE   2  (bases 1 to 10057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-08-7"; volume: "9(1):3145. doi"; pages: " 
            10.1038/s41467-018-05557-y"
COMMENT     Sequence Label: pLJM1-Clover-Prx2-mRuby2
FEATURES             Location/Qualifiers
     source          1..10057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(417..438)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1025..1046
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2385..3095
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     misc_feature    3140..3257
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3306..3816
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3735..3753
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3838..4437
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3838..3857)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4334..4354
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4565..4798
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4870..4991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4907..4926)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4961..4980
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5031..5166
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5186..5205)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5187..5205)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5215..5232)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5215..5231)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5224..5246)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5373..5828
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5460..5479)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5670..5691
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5854..5958
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5959..6027,6028..6819)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6177..6196)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6990..7578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7479..7498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7732..7749
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7866..7887
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7902..7907,7908..7925,7926..7932)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7940..7956
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7945..7967
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7964..7980
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7964..7980
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7999..8019
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8001..8019
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8047..8273
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8274..8454
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8501..8626
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9119..9352
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9537..9581
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     9879..9899
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      421 tcgagctgga cggcgacgta aacggccaca agttcagcgt ccgcggcgag ggcgagggcg
      481 atgccaccaa cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      541 cctggcccac cctcgtgacc accttcggct acggcgtggc ctgcttcagc cgctaccccg
      601 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      661 gcaccatctc tttcaaggac gacggtacct acaagacccg cgccgaggtg aagttcgagg
      721 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
      781 tcctggggca caagctggag tacaacttca acagccacaa cgtctatatc acggccgaca
      841 agcagaagaa cggcatcaag gctaacttca agatccgcca caacgttgag gacggcagcg
      901 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      961 ccgacaacca ctacctgagc catcagtccg ccctgagcaa agaccccaac gagaagcgcg
     1021 atcacatggt cctgctggag ttcgtgaccg ccagcggtgg tggtagcatg gcctccggta
     1081 acgcgcgcat cggaaagcca gcccctgact tcaaggccac agcggtggtt gatggcgcct
     1141 tcaaagaggt gaagctgtcg gactacaaag ggaagtacgt ggtcctcttt ttctaccctc
     1201 tggacttcac ctttgtgtgc cccaccgaga tcatcgcgtt cagcaaccgt gcagaggact
     1261 tccgcaagct gggctgtgaa gtgctgggcg tctcggtgga ctctcagttc acccacctgg
     1321 cttggatcaa caccccccgg aaagagggag gcttgggccc cctgaacatc cccctgcttg
     1381 ctgacgtgac cagacgcttg tctgaggatt acggcgtgct gaaaacagat gagggcattg
     1441 cctacagggg cctctttatc atcgatggca agggtgtcct tcgccagatc actgttaatg
     1501 atttgcctgt gggacgctcc gtggatgagg ctctgcggct ggtccaggcc ttccagtaca
     1561 cagacgagca tggggaagtt tgtcccgctg gctggaagcc tggcagtgac acgattaagc
     1621 ccaacgtgga tgacagcaag gaatatttct ccaaacacaa tagcggcagc ggcagcggca
     1681 gcggcagcgg cagcggcagc ggcagcgcag cagcaggttc tggtgaattt agcggcagcg
     1741 gcagcggcag cggcagcggc agcggcagcg gcagcatggc ctccggtaac gcgcgcatcg
     1801 gaaagccagc ccctgacttc aaggccacag cggtggttga tggcgccttc aaagaggtga
     1861 agctgtcgga ctacaaaggg aagtacgtgg tcctcttttt ctaccctctg gacttcacct
     1921 ttgtgtgccc caccgagatc atcgcgttca gcaaccgtgc agaggacttc cgcaagctgg
     1981 gctgtgaagt gctgggcgtc tcggtggact ctcagttcac ccacctggct tggatcaaca
     2041 ccccccggaa agagggaggc ttgggccccc tgaacatccc cctgcttgct gacgtgacca
     2101 gacgcttgtc tgaggattac ggcgtgctga aaacagatga gggcattgcc tacaggggcc
     2161 tctttatcat cgatggcaag ggtgtccttc gccagatcac tgttaatgat ttgcctgtgg
     2221 gacgctccgt ggatgaggct ctgcggctgg tccaggcctt ccagtacaca gacgagcatg
     2281 gggaagtttg tcccgctggc tggaagcctg gcagtgacac gattaagccc aacgtggatg
     2341 acagcaagga atatttctcc aaacacaata gcggtggtgg tagcatggtg tctaagggcg
     2401 aagagctgat caaggaaaat atgcgtatga aggtggtcat ggaaggttcg gtcaacggcc
     2461 accaattcaa atgcacaggt gaaggagaag gcaatccgta catgggaact caaaccatga
     2521 ggatcaaagt catcgaggga ggacccctgc catttgcctt tgacattctt gccacgtcgt
     2581 tcatgtatgg cagccgtact tttatcaagt acccgaaagg cattcctgat ttctttaaac
     2641 agtcctttcc tgagggtttt acttgggaaa gagttacgag atacgaagat ggtggagtcg
     2701 tcaccgtcat gcaggacacc agccttgagg atggctgtct cgtttaccac gtccaagtca
     2761 gaggggtaaa ctttccctcc aatggtcccg tgatgcagaa gaagaccaag ggttgggagc
     2821 ctaatacaga gatgatgtat ccagcagatg gtggtctgag gggatacact catatggcac
     2881 tgaaagttga tggtggtggc catctgtctt gctctttcgt aacaacttac aggtcaaaaa
     2941 agaccgtcgg gaacatcaag atgcccggta tccatgccgt tgatcaccgc ctggaaaggt
     3001 tagaggaaag tgacaatgaa atgttcgtag tacaacgcga acacgcagtt gccaagttcg
     3061 ccgggcttgg tggtgggatg gacgagctgt acaagtaaga attctcgacc tcgagacaaa
     3121 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     3181 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     3241 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggccgcggc
     3301 tcgagggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
     3361 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
     3421 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
     3481 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
     3541 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
     3601 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
     3661 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
     3721 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
     3781 tccctcgttg accgaatcac cgacctctct ccccaggggg atccaccgga gcttaccatg
     3841 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     3901 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     3961 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     4021 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     4081 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     4141 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     4201 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     4261 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     4321 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     4381 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     4441 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcggtacct
     4501 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     4561 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     4621 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     4681 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     4741 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     4801 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     4861 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4921 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4981 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     5041 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5101 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5161 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     5221 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     5281 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     5341 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     5401 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5461 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5521 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5581 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5641 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5701 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5761 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     5821 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5881 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5941 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6001 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6061 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6121 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6181 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6241 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6301 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6361 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6421 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6481 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6541 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6601 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6661 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6721 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6781 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6841 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6901 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6961 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7021 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7081 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7141 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7201 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7261 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7321 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7381 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7441 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7501 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7561 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7621 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7681 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7741 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7801 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7861 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7921 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7981 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     8041 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     8101 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     8161 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     8221 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     8281 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8341 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8401 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     8461 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     8521 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     8581 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     8641 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     8701 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     8761 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     8821 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     8881 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     8941 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     9001 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     9061 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     9121 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     9181 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     9241 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     9301 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     9361 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     9421 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     9481 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     9541 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     9601 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     9661 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     9721 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     9781 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     9841 gatcacgaga ctagcctcga gcggccgccc ccttcaccga gggcctattt cccatgattc
     9901 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg
     9961 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
    10021 ttgcagtttt aaaattatgt tttaaaatgg actatca
//
