LOCUS       sequence-236166-        5897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the fluorescent protein mNeonGreen in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5897)
  TITLE     AAV expression plasmids for production of mNeonGreen
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-UbC-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..5897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        150..549
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1119..1138
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     1839..1858
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1882..1891
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2143..2152
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2644..3120
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2843..2862)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3160..3300
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3160..3289
                     /label=AAV2 ITR
     rep_origin      3375..3830
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3462..3481)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3672..3693
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3847..3866)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3966..3988
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4026..4044)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4112..4216
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4217..4285,4286..5077)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4435..4454)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5248..5836
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5737..5756
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgcg gcctccgcgc cgggttttgg cgcctcccgc
      181 gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc
      241 tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga
      301 accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt
      361 tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga
      421 gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca
      481 gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg
      541 tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc
      601 ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg
      661 ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga
      721 atagaacctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
      781 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
      841 gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc
      901 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
      961 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
     1021 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
     1081 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
     1141 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcacctttga aatgtaatca
     1201 tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa attctggccg
     1261 tttttggctt ttttgttaga cgaagctaag gcgcgcctga gaacttcagg gtgagtttgg
     1321 ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat ggagggggca
     1381 aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat gcatggaccc
     1441 tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt
     1501 ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta acgaatcgat
     1561 tctagacaat cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat
     1621 aattaaatga taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct
     1681 tattggtaga aacaactaca tcctggtcat catcctgcct ttctctttat ggttacaatg
     1741 atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa
     1801 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     1861 gtctcatcaa agcttggatc cgccaccatg gtttccaagg gcgaagagga caatatggcc
     1921 agcctgcctg ccacacacga gctgcacatc tttggcagca tcaacggcgt ggacttcgac
     1981 atggttggac aaggcaccgg caatcccaac gacggctacg aggaactgaa cctgaagtcc
     2041 acaaagggcg acctgcagtt cagcccttgg attctggtgc ctcacatcgg ctacggcttc
     2101 caccagtacc tgccttatcc tgacggcatg agcccttttc aggccgccat ggtggatggc
     2161 tctggctacc aggttcaccg gaccatgcag tttgaagatg gcgccagcct gaccgtgaac
     2221 tacagataca catacgaggg cagccacatc aaaggcgagg cccaagtgaa aggcacaggc
     2281 tttccagccg atggacccgt gatgaccaat agcctgacag ccgccgactg gtgcagaagc
     2341 aagaaaacat accccaacga caagaccatc atcagcacct tcaagtggtc ctacaccacc
     2401 ggcaacggca agagatacag aagcaccgcc agaaccacct acaccttcgc caaacctatg
     2461 gccgccaact acctgaagaa ccagcctatg tacgtgttcc gcaagaccga gctgaagcac
     2521 agcaagacag aactgaactt caaagagtgg cagaaagcct tcaccgacgt gatgggcatg
     2581 gacgagctgt ataagtaggc tagcctcgag tctagagtta acggcatatt ggccagatcc
     2641 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     2701 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     2761 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     2821 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     2881 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     2941 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     3001 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     3061 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     3121 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
     3181 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     3241 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
     3301 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     3361 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3421 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3481 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3541 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     3601 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3661 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta
     3721 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     3781 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     3841 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     3901 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     3961 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     4021 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     4081 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4141 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4201 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4261 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4321 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4381 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     4441 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4501 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4561 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4621 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4681 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4741 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4801 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4861 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4921 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4981 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5041 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5101 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5161 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5221 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5281 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5341 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     5401 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5461 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5521 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5581 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5641 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5701 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5761 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5821 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5881 gccttttgct cacatgt
//
