LOCUS       sequence-236173-        2885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for mScarlet-I-pdt3 (fast-folding
            mScarlet variant, codon-optimized for E. col, with pdt3 degradation 
            tag; see https://www.fpbase.org/protein/mscarlet-i/)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2885)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C108m
FEATURES             Location/Qualifiers
     source          1..2885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..25
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(29..72)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    75..96
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    903..923
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(929..946)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1100..1688)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1180..1199)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1859..2650,2651..2719))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2482..2501
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2720..2824)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
       61 aaggatgatt tctggaattc gcggccgctt ctagagacta gtgggtctca aatggtcagt
      121 aaaggcgaag cagttatcaa agagttcatg cgcttcaaag ttcatatgga agggtcgatg
      181 aacgggcacg aatttgaaat tgaaggcgaa ggcgaaggcc gcccatatga agggacccaa
      241 accgcaaagc ttaaggttac taaaggcggt ccattaccct tttcgtggga cattttaagc
      301 ccacagttta tgtacgggag tcgcgctttc atcaagcacc ctgcggacat cccagattac
      361 tacaaacagt ctttccccga ggggttcaag tgggagcgcg tgatgaactt cgaggatggc
      421 ggagccgtga cggtcaccca agatacctct ttggaggacg gtacgttgat ctacaaagtg
      481 aaattgcgtg gcacgaattt tccacctgat gggcctgtca tgcagaaaaa gacaatggga
      541 tgggaagctt ccacggagcg cctttaccca gaggacggtg ttcttaaagg ggatatcaaa
      601 atggcgctgc gtcttaaaga tggaggccgc tacctggcgg acttcaagac tacttacaag
      661 gccaaaaaac cagtgcagat gccgggtgcg tacaatgtag atcgtaaatt agatattaca
      721 agtcacaatg aagattacac ggtcgtagag cagtatgagc gcagtgaggg gcgtcactct
      781 acgggcggta tggacgagtt atacaaggcg gcgaacaaaa acgaagaaaa caccaacgaa
      841 gtgccgacct ttatgctgaa cgcgggccag gcgaacagaa gacgagttta aaggtagaga
      901 cctactagta gcggccgctg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
      961 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1021 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1081 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc
     1141 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1201 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1261 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1321 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1381 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1441 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1501 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1561 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1621 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1681 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1741 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1801 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1861 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1921 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1981 gtgctgcaat gataccgcgc gacccacgct caccggctcc agatttatca gcaataaacc
     2041 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2101 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2161 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2221 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2281 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2341 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2401 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2461 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2521 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2581 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2641 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2701 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2761 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2821 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     2881 taacc
//
