LOCUS       sequence-236179-        2142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly BC part for UTR1 (very strong RBS). 
            Please see Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2142)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U4m
FEATURES             Location/Qualifiers
     source          1..2142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4..22
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(26..69)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    72..93
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             118..140
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    157..177
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(183..200)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(354..942)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(434..453)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1113..1904,1905..1973))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1736..1755
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1974..2078)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
       61 gatgatttct ggaattcgcg gccgcttcta gagactagtg ggtctcatac taataatttt
      121 gtttaacttt aagaaggaga tatacaatga gagacctact agtagcggcc gctgcaggct
      181 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      241 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      301 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac
      361 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      421 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      481 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      541 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      601 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      661 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      721 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      781 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      841 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      901 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      961 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1021 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1081 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1141 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1201 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgcgaccca
     1261 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1321 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1381 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1441 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1501 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1561 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1621 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1681 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1741 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1801 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1861 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1921 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1981 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2041 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2101 cctgacgtct aagaaaccat tattatcatg acattaacct at
//
