LOCUS       sequence-236181-        8924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BvCas12b in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, 
            Makarova KS, Koonin EV, Zhang F
  TITLE     Engineering of CRISPR-Cas12b for human genome editing.
  JOURNAL   Nat Commun. 2019 Jan 22;10(1):212. doi: 10.1038/s41467-018-08224-4.
  PUBMED    30670702
REFERENCE   2  (bases 1 to 8924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-22"; volume: "10(1):212. doi"; pages: " 
            10.1038/s41467-018-08224-4"
COMMENT     Sequence Label: pZ143-pcDNA3-BvCas12b
FEATURES             Location/Qualifiers
     source          1..8924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      683..692
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             695..715
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4073..4120
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4127..4207
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4281..4298)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4287..4511
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4557..4985
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4644..4663)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4854..4875
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4994..5014)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4999..5328
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5179..5314
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5241..5260
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5395..6189
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5449..5468)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6059..6078
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6363..6484
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6400..6419)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6454..6473
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6533..6549)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6533..6549)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6546..6568)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6557..6573
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6581..6587,6588..6605,6606..6611))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6626..6647
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6764..6781)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6935..7523)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7015..7034)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7694..8485,8486..8554))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8317..8336
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8555..8659)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8734..8753)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttgccaccat ggccccaaag aagaagcgga aggtcggtat
      721 ccacggagtc ccagcagccg ccatccggtc catcaagctg aagatgaaga ccaacagcgg
      781 caccgacagc atctacctga gaaaagccct gtggcggacc caccagctga tcaatgaggg
      841 aatcgcctac tacatgaacc tgctgaccct gtaccggcaa gaggccatcg gcgacaagac
      901 caaagaagcc tatcaggccg agctgattaa catcatccgg aaccagcagc ggaacaacgg
      961 cagctctgag gaacacggct ccgaccaaga aattctggcc ctgctgagac agctgtacga
     1021 gctgatcatc cccagcagca tcggcgaatc tggcgacgct aatcagctgg gcaacaagtt
     1081 tctgtaccct ctggtggacc ccaacagcca gtctggcaag ggcacatcta acgccggcag
     1141 aaagcccaga tggaagcggc tgaaagagga aggcaacccc gactgggaac tcgagaagaa
     1201 gaaggacgag gaacgcaagg ccaaggatcc caccgtgaag atctttgaca acctgaacaa
     1261 atacggcctg ctgcctctgt tcccactgtt caccaacatc cagaaagaca tcgagtggct
     1321 gcccctgggc aagagacagt ctgtgcggaa gtgggacaaa gacatgttca tccaggccat
     1381 cgagagactg ctgagctggg agagctggaa cagaagagtg gccgacgagt acaaacagct
     1441 gaaagaaaag accgagagct actacaaaga gcacctgaca ggcggcgagg aatggatcga
     1501 gaagatccgg aagttcgaga aagaacggaa catggaactg gaaaagaacg ccttcgctcc
     1561 caacgacggc tacttcatca ccagcagaca gatcagaggc tgggacagag tgtacgagaa
     1621 gtggtccaag ctgcccgagt ctgctagccc tgaggaactg tggaaagtgg tggccgagca
     1681 gcagaacaag atgtccgaag gcttcggcga ccccaaggtg ttcagcttcc tggccaacag
     1741 agagaaccgg gacatttgga gaggccacag cgagcggatc taccacattg ccgcctacaa
     1801 cggcctgcag aagaagctga gccggaccaa agagcaggcc accttcacac tgcctgacgc
     1861 cattgaacac cctctgtgga tcagatacga gagccctggc ggcaccaacc tgaatctgtt
     1921 caagctggaa gagaaacaga aaaagaacta ctacgtgacc ctgagcaaga tcatctggcc
     1981 cagcgaggaa aagtggattg agaaagagaa catcgagatc cctctggctc ccagcatcca
     2041 gttcaaccgg cagattaagc tgaagcagca cgtgaagggc aagcaagaga tcagcttcag
     2101 cgactacagc agccggatca gcctggatgg tgttctcggc ggcagcagaa tccagtttaa
     2161 tcggaagtac atcaagaacc acaaagagct gctcggagag ggcgacatcg gccccgtgtt
     2221 ctttaacctg gtggtggatg tggcccctct gcaagaaacc agaaacggca gactgcagag
     2281 ccccatcggc aaggccctga aagtgatcag cagcgacttc tccaaagtga tcgactacaa
     2341 gccgaaagaa ctcatggatt ggatgaatac cggcagcgcc agcaacagct ttggagtggc
     2401 ttctctgctg gaaggcatga gagtgatgag catcgacatg ggccagagaa ccagcgcctc
     2461 cgtgtccatc ttcgaggtcg tgaaagaact gcccaaggat caagagcaga agctgttcta
     2521 cagcatcaac gacaccgagc tgttcgccat ccacaagcgg agctttctgc tgaacctgcc
     2581 tggcgaggtg gtcaccaaga acaacaagca gcagcggcaa gagcggcgga aaaagcggca
     2641 gtttgtgcgg agccagatca gaatgctggc caacgtgctg cggctggaaa caaagaaaac
     2701 ccctgacgag cggaagaagg ccattcacaa gctgatggaa atcgtgcaga gctacgacag
     2761 ctggaccgcc agccagaaag aagtgtggga gaaagagctg aatctcctga ccaacatggc
     2821 cgccttcaat gacgagatct ggaaagaaag cctggtggaa ctgcaccacc ggatcgagcc
     2881 ttacgtggga cagatcgtgt ccaagtggcg gaagggcctg tctgagggca gaaagaatct
     2941 ggccggcatc agcatgtgga acatcgacga actggaagat accaggcggc tgctgatttc
     3001 ctggtccaag agaagcagaa ccccaggcga ggccaacagg atcgaaaccg atgagccttt
     3061 cggcagcagc ctgctccagc acattcagaa cgtgaaggac gacagactga agcagatggc
     3121 caacctgatc atcatgacag ccctgggctt taagtacgac aaagaggaaa aggaccggta
     3181 caagcggtgg aaagagacat accccgcctg ccagatcatc ctgttcgaga acctgaaccg
     3241 ctacctgttc aacctcgacc ggtccagacg cgagaacagc agactgatga agtgggccca
     3301 tcggagcatc cccagaaccg tgtctatgca gggcgagatg ttcggcctgc aagtgggcga
     3361 cgttcggagc gagtacagct ccagattcca cgccaaaaca ggcgcccctg gcatcagatg
     3421 tcacgccctg actgaagagg atctgaaggc cggcagcaac accctgaaga gactgatcga
     3481 ggacggcttc atcaatgaga gcgagctggc ctacctgaag aagggcgata tcatccctag
     3541 ccaaggcggc gaactgttcg tgacactgtc caagcggtac aagaaggaca gcgacaacaa
     3601 cgagctgacc gtgatccacg ccgacatcaa cgccgctcag aatctgcaga agcggttttg
     3661 gcagcaaaac agcgaggtgt acagagtgcc ctgtcagctg gccagaatgg gcgaagataa
     3721 gctgtacatc cccaagagcc agaccgagac aatcaagaag tatttcggca agggctcctt
     3781 cgtgaagaac aataccgaac aagaggtcta caagtgggag aagtccgaga aaatgaagat
     3841 caagacggac accaccttcg acctgcaaga cctggatggc ttcgaggaca tcagcaagac
     3901 cattgagctg gcacaagagc agcaaaagaa atacctgacc atgttcaggg accccagcgg
     3961 ctactttttc aacaatgaga catggcggcc tcaaaaagaa tactggtcca tcgtgaacaa
     4021 catcatcaag agctgcctca agaagaagat cctgagcaac aaggtcgagc tgaaaaggcc
     4081 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctacc catacgatgt
     4141 tccagattac gcttatccct acgacgtgcc tgattatgca tacccatatg atgtccccga
     4201 ctatgcctaa gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc
     4261 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     4321 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     4381 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     4441 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     4501 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     4561 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4621 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4681 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4741 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4801 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4861 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4921 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4981 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5041 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5101 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5161 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5221 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     5281 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     5341 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     5401 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     5461 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     5521 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     5581 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     5641 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     5701 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     5761 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     5821 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     5881 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     5941 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     6001 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     6061 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     6121 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     6181 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     6241 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     6301 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     6361 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6421 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6481 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     6541 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6601 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6661 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6721 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6781 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6841 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6901 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6961 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7021 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7081 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7141 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7201 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7261 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7321 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7381 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7441 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7501 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7561 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7621 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7681 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7741 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7801 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7861 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7921 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7981 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8041 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8101 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8161 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8221 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8281 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8341 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8401 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8461 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8521 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8581 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8641 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     8701 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     8761 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     8821 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     8881 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt
//
