LOCUS       sequence-236184-        2192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly BC part for BCD24 (low expression 
            bi-cistronic RBS, engineered for downstream context-independence; 
            see https://doi.org/10.1038/nmeth.2404)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2192)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U8m
FEATURES             Location/Qualifiers
     source          1..2192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..19
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(23..66)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    69..90
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    204..224
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(230..247)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(401..989)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(481..500)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1160..1951,1952..2020))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1783..1802
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2021..2125)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
       61 gatttctgga attcgcggcc gcttctagag actagtgggt ctcatactgg gcccaagttc
      121 acttaaaaag gagatcaaca atgaaagcaa ttttcgtact gaaacatctt aatcatgcga
      181 tggacggttt ctaatgagag acctactagt agcggccgct gcaggcttcc tcgctcactg
      241 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      301 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      361 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc
      421 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      481 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      541 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      601 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      661 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      721 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      781 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      841 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      901 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      961 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1021 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1081 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1141 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1201 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1261 agggcttacc atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc
     1321 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1381 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1441 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1501 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1561 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1621 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1681 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1741 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1801 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1861 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1921 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1981 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     2041 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2101 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     2161 aaaccattat tatcatgaca ttaacctata aa
//
