LOCUS       sequence-236187-        9494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for stable expression of Firefly luciferase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Gorbea C, Mosbruger T, Nix DA, Cazalla D
  TITLE     Viral miRNA adaptor differentially recruits miRNAs to target mRNAs 
            through alternative base-pairing.
  JOURNAL   Elife. 2019 Sep 20;8. pii: 50530. doi: 10.7554/eLife.50530.
  PUBMED    31538617
REFERENCE   2  (bases 1 to 9494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-09-20"; volume: "8. pii: 50530. doi"; pages: " 
            10.7554/eLife.50530"
COMMENT     Sequence Label: pLenti-TK-Firefly-cjYTHDC1
FEATURES             Location/Qualifiers
     source          1..9494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     223..246
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        293..1044
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             1051..2703
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3036..3053)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3042..3266
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3502..3546)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3731..3964
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4457..4582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4629..4809
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4810..5036)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(5064..5084)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5064..5082)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5103..5119)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5103..5119)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5116..5138)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5127..5143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5151..5157,5158..5175,5176..5181))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5196..5217
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5334..5351)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5505..6093)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5585..5604)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6264..7055,7056..7124))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6887..6906
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7125..7229)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7255..7710)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7392..7413)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7604..7623
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7837..7859
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7851..7868
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7852..7868
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7878..7897
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7878..7896
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7917..8052
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8092..8213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8103..8122)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8157..8176
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             8285..8518
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(8656..9255)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8739..8759)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9236..9255
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(join(9276..9494,1..281))
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9324..9343)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ttgctcagcg gtgctgtcca tctgcacgag actagtgaga cgtgctactt ccatttgtca
       61 cgtcctgcac gacgcgagct gcggggcggg ggggaacttc ctgactaggg gaggagtaga
      121 aggtggcgcg aaggggccac caaagaacgg agccggttgg cgcctaccgg tggatgtgga
      181 atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc
      241 gcatgctcca gactgccttg ggaaaagcgc ctcccctacc cggtagaatt gaaatgagtc
      301 ttcggacctc gcgggggccg cttaagcggt ggttagggtt tgtctgacgc ggggggaggg
      361 ggaaggaacg aaacactctc attcggaggc ggctcggggt ttggtcttgg tggccacggg
      421 cacgcagaag agcgccgcga tcctcttaag cacccccccg ccctccgtgg aggcgggggt
      481 ttggtcggcg ggtggtaact ggcgggccgc tgactcgggc gggtcgcgcg ccccagagtg
      541 tgaccttttc ggtctgctcg cagacccccg ggcggcgccg ccgcggcggc gacgggctcg
      601 ctgggtccta ggctccatgg ggaccgtata cgtggacagg ctctggagca tccgcacgac
      661 tgcggtgata ttaccggaga ccttctgcgg gacgagccgg gtcacgcggc tgacgcggag
      721 cgtccgttgg gcgacaaaca ccaggacggg gcacaggtac actatcttgt cacccggagg
      781 cgcgagggac tgcaggagct tcagggagtg gcgcagctgc ttcatccccg tggcccgttg
      841 ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg catgtcttta gttctatgat
      901 gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg
      961 gcggcgcggt cccaggtcca cttcgcatat taaggtgacg cgtgtggcct cgaacaccga
     1021 gcgaccctgc agcgacccgc ttaaggatcc atggaagatg ccaaaaacat taagaagggc
     1081 ccagcgccat tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg
     1141 aagcgctacg ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac
     1201 attacctacg ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat
     1261 gggctgaata caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg
     1321 cccgtgttgg gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac
     1381 gagcgcgagc tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag
     1441 aaagggctgc aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc
     1501 atcatggata gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc
     1561 catttgccac ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa
     1621 accatcgccc tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta
     1681 ccgcaccgca ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag
     1741 atcatccccg acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc
     1801 accacgctgg gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag
     1861 gagctattct tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca
     1921 ctatttagct tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac
     1981 gagatcgcca gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc
     2041 ttccacctac caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg
     2101 atcacccccg aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag
     2161 gctaaggtgg tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg
     2221 tgcgtccgtg gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct
     2281 ctcatcgaca aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag
     2341 cacttcttca tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc
     2401 ccagccgaac tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc
     2461 ggcctgcccg acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt
     2521 aaaaccatga ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag
     2581 aagctgcgcg gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg
     2641 gacgcccgca agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg
     2701 taattctagt tgtttaaacc gagagcgaga ccgccctaga gataacagaa gagacagaga
     2761 gcgagataga ggacgtgata gagaaagaga aagagagcga ttatgtgatc gggacagaga
     2821 ccgaggggag agaggtcgat atcgaagata atccgctttt ggaagcaatg attgtttaaa
     2881 gatacaaaaa atcttgtatt ttttttgtgt gtgtttacaa gtagtaaatt tattttcagc
     2941 tgtctaccta taaagttcat tgtgtagaag gatttattat gacccccctt tgttccaagc
     3001 atgctctaga gtcgacgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     3061 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3121 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     3181 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     3241 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     3301 ggggtatccc ctcccccctt ttcttttggg tcccctcggg gttgggaggt gggtctgaaa
     3361 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
     3421 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
     3481 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
     3541 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
     3601 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
     3661 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
     3721 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
     3781 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
     3841 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
     3901 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
     3961 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
     4021 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
     4081 cctcatatct cctcctccag gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac
     4141 ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc
     4201 tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc
     4261 ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta
     4321 acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta
     4381 atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc
     4441 tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca
     4501 aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa
     4561 gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg
     4621 ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     4681 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     4741 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     4801 gagagacccg tttatgtatc gagctaggca cttaaataca atatctctgc aatgcggcaa
     4861 ttcagtggtt cgtccaatcc atgtcagacc cgtctgttgc cttcctaata aggcacgatc
     4921 gtaccacctt acttccacca atcggcatgc acggtgcttt ttctctcctt gtaaggcatg
     4981 ttgctaactc atcgttacca tgttgcaaga ctacaagagt attgcataag actacattaa
     5041 gcttgcagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     5101 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     5161 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     5221 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5281 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5341 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5401 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5461 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5521 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5581 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5641 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5701 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5761 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5821 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5881 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5941 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6001 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6061 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6121 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6181 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6241 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6301 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6361 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6421 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6481 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6541 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6601 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6661 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6721 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6781 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6841 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6901 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6961 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7021 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7081 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7141 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7201 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     7261 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     7321 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     7381 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     7441 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     7501 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     7561 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     7621 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     7681 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     7741 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     7801 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     7861 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacg tccctaggcc
     7921 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     7981 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     8041 taggggcggg atgggcggag ttaggggcgg gatagctaga gccagacatg ataagataca
     8101 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     8161 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttcctctca
     8221 ctctctgata ttcatttctt tgcaagttat aaatactgaa taataagatg acatgaacta
     8281 ctactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     8341 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     8401 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     8461 gacccagtac aagcaaaaag cagatcttgt cttcgttggg agtgagctag cccttccagt
     8521 cccccctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt
     8581 aaaggtaccg ggccctcgag atgcatgggg tcgtgcgctc ctttcggtcg ggcgctgcgg
     8641 gtcgtggggc gggcgtcagg caccgggctt gcgggtcatg caccaggtgc gcggtccttc
     8701 gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg
     8761 gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg
     8821 gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc
     8881 caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg
     8941 cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc
     9001 ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac
     9061 ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac
     9121 ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt cggcgaacgc
     9181 ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc gcaccgtggg
     9241 cttgtactcg gtcatggtaa gcttgggctg caggtcgaaa ggcccggaga tgaggaagag
     9301 gagaacagcg cggcagacgt gcgcttttga agcgtgcaga atgccgggcc tccggaggac
     9361 cttcgggcgc ccgccccgcc cctgagcccg cccctgagcc cgcccccgga cccacccctt
     9421 cccagcctct gagcccagaa agcgaaggag caaagctgct attggccgct gccccaaagg
     9481 cctacccgct tcca
//
